ID   ORD_DROME               Reviewed;         479 AA.
AC   Q24434; Q9W1S8;
DT   19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2003, sequence version 2.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Protein ORD;
DE   AltName: Full=Orientation disrupter protein;
GN   Name=ord; ORFNames=CG3134;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis;
RX   PubMed=8635478; DOI=10.1002/j.1460-2075.1996.tb00487.x;
RA   Bickel S.E., Wyman D.W., Miyazaki W.Y., Moore D.P., Orr-Weaver T.L.;
RT   "Identification of ORD, a Drosophila protein essential for sister chromatid
RT   cohesion.";
RL   EMBO J. 15:1451-1459(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=12062057; DOI=10.1016/s0960-9822(02)00846-1;
RA   Bickel S.E., Orr-Weaver T.L., Balicky E.M.;
RT   "The sister-chromatid cohesion protein ORD is required for chiasma
RT   maintenance in Drosophila oocytes.";
RL   Curr. Biol. 12:925-929(2002).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=12429833; DOI=10.1091/mbc.e02-06-0332;
RA   Balicky E.M., Endres M.W., Lai C., Bickel S.E.;
RT   "Meiotic cohesion requires accumulation of ORD on chromosomes before
RT   condensation.";
RL   Mol. Biol. Cell 13:3890-3900(2002).
RN   [6]
RP   INTERACTION WITH SCE.
RX   PubMed=14669021; DOI=10.1007/s00412-003-0266-0;
RA   Balicky E.M., Young L., Orr-Weaver T.L., Bickel S.E.;
RT   "A proposed role for the Polycomb group protein dRING in meiotic sister-
RT   chromatid cohesion.";
RL   Chromosoma 112:231-239(2004).
CC   -!- FUNCTION: Essential for proper maintenance of sister-chromatid cohesion
CC       in both male and female meiosis. Mutations in ord cause premature
CC       separation of the sister chromatids in meiosis I and random segregation
CC       in both meiotic divisions. Required for chiasma maintenance in female
CC       meiosis. Mutations in ord reduce recombination in female meiosis.
CC   -!- SUBUNIT: Interacts with Sce. {ECO:0000269|PubMed:14669021}.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Chromosome. Chromosome, centromere.
CC       Note=In male meiosis it assembles onto chromosomes in late G2 of the
CC       meiotic cell cycle. It remains at the centromeres after chromosome
CC       condensation, through the meiosis I division until the separation of
CC       sister chromatids at anaphase II.
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DR   EMBL; X92840; CAA63426.1; -; mRNA.
DR   EMBL; AE013599; AAF46975.1; -; Genomic_DNA.
DR   PIR; S66578; S66578.
DR   RefSeq; NP_001286760.1; NM_001299831.1.
DR   RefSeq; NP_726303.1; NM_166589.2.
DR   AlphaFoldDB; Q24434; -.
DR   BioGRID; 63290; 2.
DR   IntAct; Q24434; 1.
DR   STRING; 7227.FBpp0071956; -.
DR   PaxDb; Q24434; -.
DR   DNASU; 37687; -.
DR   EnsemblMetazoa; FBtr0072047; FBpp0071956; FBgn0003009.
DR   EnsemblMetazoa; FBtr0343199; FBpp0309889; FBgn0003009.
DR   GeneID; 37687; -.
DR   KEGG; dme:Dmel_CG3134; -.
DR   CTD; 37687; -.
DR   FlyBase; FBgn0003009; ord.
DR   VEuPathDB; VectorBase:FBgn0003009; -.
DR   eggNOG; ENOG502TD0Q; Eukaryota.
DR   HOGENOM; CLU_566559_0_0_1; -.
DR   InParanoid; Q24434; -.
DR   OMA; WICHRYL; -.
DR   OrthoDB; 700126at2759; -.
DR   PhylomeDB; Q24434; -.
DR   BioGRID-ORCS; 37687; 0 hits in 1 CRISPR screen.
DR   ChiTaRS; shf; fly.
DR   GenomeRNAi; 37687; -.
DR   PRO; PR:Q24434; -.
DR   Proteomes; UP000000803; Chromosome 2R.
DR   Bgee; FBgn0003009; Expressed in testis and 5 other tissues.
DR   ExpressionAtlas; Q24434; baseline and differential.
DR   Genevisible; Q24434; DM.
DR   GO; GO:0000785; C:chromatin; IDA:UniProtKB.
DR   GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:UniProtKB.
DR   GO; GO:0001674; C:female germ cell nucleus; IDA:UniProtKB.
DR   GO; GO:0001673; C:male germ cell nucleus; IDA:UniProtKB.
DR   GO; GO:0005635; C:nuclear envelope; IDA:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0007059; P:chromosome segregation; IMP:FlyBase.
DR   GO; GO:0007066; P:female meiosis sister chromatid cohesion; IDA:UniProtKB.
DR   GO; GO:0007276; P:gamete generation; IMP:FlyBase.
DR   GO; GO:0051457; P:maintenance of protein location in nucleus; IMP:UniProtKB.
DR   GO; GO:0007060; P:male meiosis chromosome segregation; IMP:FlyBase.
DR   GO; GO:0007065; P:male meiosis sister chromatid cohesion; IDA:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IMP:FlyBase.
DR   GO; GO:0051177; P:meiotic sister chromatid cohesion; IMP:FlyBase.
DR   GO; GO:0000278; P:mitotic cell cycle; IMP:FlyBase.
DR   GO; GO:0007062; P:sister chromatid cohesion; IMP:FlyBase.
PE   1: Evidence at protein level;
KW   Centromere; Chromosome; Meiosis; Nucleus; Reference proteome.
FT   CHAIN           1..479
FT                   /note="Protein ORD"
FT                   /id="PRO_0000058080"
FT   REGION          102..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        444
FT                   /note="D -> E (in Ref. 1; CAA63426)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   479 AA;  55166 MW;  D2149D0325E2497D CRC64;
     MYGETTLNKN HVIKFIKLKI NNCLGCDEVE INFSKADNVH IIIYSLVTDM AKDLPAVTPV
     AQAILLLCSL TYPDSDSLET IPQLKIGKGS VSMSFKVYPV NKEEETEPES ESDLDEGPST
     SKQALERMVQ RAERKAKEAS TRNVHSKGIY VNVERRFDMY FALDTVSYYI NGGKRQSCPL
     PEFHAKFFVR PQHSINLLRQ LHEKCSGNWL KVIQSDGDGD AFKKFKDPDS PFETFVKLFE
     SNPIKPNDMM GKLAKTCLHV NEAVRLTERE FILEVFNQVR HIFEYITAQE YTVWFLVPCL
     GDKDQLRSKT LEDFDLTKVR TSIRRAGDTS NIWWDHTDHN IKDILLVAFQ LDLATHVNQS
     VLVISHLETL AEFSTMQYVT AFFMNDFYAK KNTEPKWICH RYLERIIDVA LFLGVIVIIE
     YPSAFTLLQE GRHLIKCFQK ENADSSRTSQ WEIFEDVVKE NESDLEFLKE AVGKVQQNV