OREX_MOUSE
ID OREX_MOUSE Reviewed; 130 AA.
AC O55241;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Orexin;
DE AltName: Full=Hypocretin;
DE Short=Hcrt;
DE Contains:
DE RecName: Full=Orexin-A;
DE AltName: Full=Hypocretin-1;
DE Short=Hcrt1;
DE Contains:
DE RecName: Full=Orexin-B;
DE AltName: Full=Hypocretin-2;
DE Short=Hcrt2;
DE Flags: Precursor;
GN Name=Hcrt; Synonyms=Ox, Ppox;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9491897; DOI=10.1016/s0092-8674(00)80949-6;
RA Sakurai T., Amemiya A., Ishii M., Matsuzaki I., Chemelli R.M., Tanaka H.,
RA Williams S.C., Richardson J.A., Kozlowski G.P., Wilson S., Arch J.R.S.,
RA Buckingham R.E., Haynes A.C., Carr S.A., Annan R.S., McNulty D.E.,
RA Liu W.-S., Terrett J.A., Elshourbagy N.A., Bergsma D.J., Yanagisawa M.;
RT "Orexins and orexin receptors: a family of hypothalamic neuropeptides and G
RT protein-coupled receptors that regulate feeding behavior.";
RL Cell 92:573-585(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=9419374; DOI=10.1073/pnas.95.1.322;
RA de Lecea L., Kilduff T.S., Peyron C., Gao X.-B., Foye P.E., Danielson P.E.,
RA Fukuhara C., Battenberg E.L.F., Gautvik V.T., Bartlett F.S. II,
RA Frankel W.N., van den Pol A.N., Bloom F.E., Gautvik K.M., Sutcliffe J.G.;
RT "The hypocretins: hypothalamus-specific peptides with neuroexcitatory
RT activity.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:322-327(1998).
RN [3]
RP REVIEW.
RX PubMed=11340621; DOI=10.1002/bies.1058;
RA Hungs M., Mignot E.;
RT "Hypocretin/orexin, sleep and narcolepsy.";
RL Bioessays 23:397-408(2001).
RN [4]
RP REVIEW.
RX PubMed=11283317; DOI=10.1146/annurev.neuro.24.1.429;
RA Willie J.T., Chemelli R.M., Sinton C.M., Yanagisawa M.;
RT "To eat or to sleep? Orexin in the regulation of feeding and wakefulness.";
RL Annu. Rev. Neurosci. 24:429-458(2001).
CC -!- FUNCTION: Neuropeptides that play a significant role in the regulation
CC of food intake and sleep-wakefulness, possibly by coordinating the
CC complex behavioral and physiologic responses of these complementary
CC homeostatic functions. A broader role in the homeostatic regulation of
CC energy metabolism, autonomic function, hormonal balance and the
CC regulation of body fluids, is also suggested. Orexin-A binds to both
CC OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R
CC with a similar high affinity.
CC -!- SUBCELLULAR LOCATION: Rough endoplasmic reticulum {ECO:0000250}.
CC Cytoplasmic vesicle {ECO:0000250}. Synapse {ECO:0000250}.
CC Note=Associated with perikaryal rough endoplasmic reticulum as well as
CC cytoplasmic large granular vesicles at synapses. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Restricted to neuronal cell bodies of the dorsal
CC and lateral hypothalamus.
CC -!- PTM: Specific enzymatic cleavages at paired basic residues yield the
CC different active peptides.
CC -!- SIMILARITY: Belongs to the orexin family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=Qui dort dine - Issue 15 of
CC October 2001;
CC URL="https://web.expasy.org/spotlight/back_issues/015";
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DR EMBL; AF041242; AAC40040.1; -; mRNA.
DR EMBL; AF019566; AAC02934.1; -; mRNA.
DR CCDS; CCDS25436.1; -.
DR RefSeq; NP_034540.1; NM_010410.2.
DR AlphaFoldDB; O55241; -.
DR STRING; 10090.ENSMUSP00000057578; -.
DR PaxDb; O55241; -.
DR PRIDE; O55241; -.
DR ProteomicsDB; 294109; -.
DR Antibodypedia; 3468; 358 antibodies from 33 providers.
DR DNASU; 15171; -.
DR Ensembl; ENSMUST00000055083; ENSMUSP00000057578; ENSMUSG00000045471.
DR GeneID; 15171; -.
DR KEGG; mmu:15171; -.
DR UCSC; uc007lmg.1; mouse.
DR CTD; 3060; -.
DR MGI; MGI:1202306; Hcrt.
DR VEuPathDB; HostDB:ENSMUSG00000045471; -.
DR eggNOG; ENOG502S83I; Eukaryota.
DR GeneTree; ENSGT00390000014272; -.
DR HOGENOM; CLU_149027_1_0_1; -.
DR InParanoid; O55241; -.
DR OMA; HPCPGRR; -.
DR OrthoDB; 1586513at2759; -.
DR PhylomeDB; O55241; -.
DR TreeFam; TF330756; -.
DR Reactome; R-MMU-389397; Orexin and neuropeptides FF and QRFP bind to their respective receptors.
DR Reactome; R-MMU-416476; G alpha (q) signalling events.
DR BioGRID-ORCS; 15171; 4 hits in 73 CRISPR screens.
DR ChiTaRS; Hcrt; mouse.
DR PRO; PR:O55241; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; O55241; protein.
DR Bgee; ENSMUSG00000045471; Expressed in dorsomedial nucleus of hypothalamus and 38 other tissues.
DR ExpressionAtlas; O55241; baseline and differential.
DR Genevisible; O55241; MM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
DR GO; GO:0098794; C:postsynapse; IEA:GOC.
DR GO; GO:0005791; C:rough endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0030141; C:secretory granule; ISO:MGI.
DR GO; GO:0005184; F:neuropeptide hormone activity; ISO:MGI.
DR GO; GO:0031771; F:type 1 hypocretin receptor binding; ISO:MGI.
DR GO; GO:0031772; F:type 2 hypocretin receptor binding; ISO:MGI.
DR GO; GO:0042755; P:eating behavior; ISO:MGI.
DR GO; GO:0060079; P:excitatory postsynaptic potential; ISO:MGI.
DR GO; GO:0008156; P:negative regulation of DNA replication; ISO:MGI.
DR GO; GO:0043267; P:negative regulation of potassium ion transport; ISO:MGI.
DR GO; GO:0051970; P:negative regulation of transmission of nerve impulse; ISO:MGI.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; ISO:MGI.
DR GO; GO:0051928; P:positive regulation of calcium ion transport; ISO:MGI.
DR GO; GO:0120162; P:positive regulation of cold-induced thermogenesis; IMP:YuBioLab.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISO:MGI.
DR GO; GO:0051971; P:positive regulation of transmission of nerve impulse; ISO:MGI.
DR GO; GO:0007205; P:protein kinase C-activating G protein-coupled receptor signaling pathway; ISO:MGI.
DR GO; GO:0046928; P:regulation of neurotransmitter secretion; ISO:MGI.
DR GO; GO:0042594; P:response to starvation; IBA:GO_Central.
DR GO; GO:0030431; P:sleep; IBA:GO_Central.
DR GO; GO:0001659; P:temperature homeostasis; ISO:MGI.
DR InterPro; IPR001704; Orexin.
DR PANTHER; PTHR15173; PTHR15173; 1.
DR Pfam; PF02072; Orexin; 1.
DR PIRSF; PIRSF037824; Orexin; 1.
DR PRINTS; PR01091; OREXINPP.
PE 2: Evidence at transcript level;
KW Amidation; Cleavage on pair of basic residues; Cytoplasmic vesicle;
KW Disulfide bond; Endoplasmic reticulum; Neuropeptide;
KW Pyrrolidone carboxylic acid; Reference proteome; Signal; Synapse.
FT SIGNAL 1..32
FT /evidence="ECO:0000250"
FT PEPTIDE 33..65
FT /note="Orexin-A"
FT /id="PRO_0000020264"
FT PEPTIDE 69..96
FT /note="Orexin-B"
FT /id="PRO_0000020265"
FT PROPEP 97..130
FT /id="PRO_0000020266"
FT MOD_RES 33
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000250|UniProtKB:O55232"
FT MOD_RES 65
FT /note="Leucine amide"
FT /evidence="ECO:0000250"
FT MOD_RES 96
FT /note="Methionine amide"
FT /evidence="ECO:0000250"
FT DISULFID 38..44
FT /evidence="ECO:0000250"
FT DISULFID 39..46
FT /evidence="ECO:0000250"
SQ SEQUENCE 130 AA; 13503 MW; D3C223F9EE835F1C CRC64;
MNFPSTKVPW AAVTLLLLLL LPPALLSLGV DAQPLPDCCR QKTCSCRLYE LLHGAGNHAA
GILTLGKRRP GPPGLQGRLQ RLLQANGNHA AGILTMGRRA GAELEPHPCS GRGCPTVTTT
ALAPRGGSGV
