ID   ORF0_VZVO               Reviewed;         221 AA.
AC   Q9E2H5;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Membrane protein 0;
DE   AltName: Full=Membrane ORF0 protein;
DE   AltName: Full=ORF S/L;
GN   ORFNames=ORF0;
OS   Varicella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=341980;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11070031; DOI=10.1128/jvi.74.23.11311-11321.2000;
RA   Kemble G.W., Annunziato P., Lungu O., Winter R.E., Cha T.A.,
RA   Silverstein S.J., Spaete R.R.;
RT   "Open reading frame S/L of varicella-zoster virus encodes a cytoplasmic
RT   protein expressed in infected cells.";
RL   J. Virol. 74:11311-11321(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Human/Japan/P-Oka/1970, and
RC   Oka varicella vaccine Biken (V-Oka-Biken);
RX   PubMed=12388706; DOI=10.1128/jvi.76.22.11447-11459.2002;
RA   Gomi Y., Sunamachi H., Mori Y., Nagaike K., Takahashi M., Yamanishi K.;
RT   "Comparison of the complete DNA sequences of the Oka varicella vaccine and
RT   its parental virus.";
RL   J. Virol. 76:11447-11459(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Oka varicella vaccine VarilRix (V-Oka-GSK), and
RC   Oka varicella vaccine Varivax (V-Oka-Merk);
RX   PubMed=18787000; DOI=10.1128/jvi.00777-08;
RA   Tillieux S.L., Halsey W.S., Thomas E.S., Voycik J.J., Sathe G.M.,
RA   Vassilev V.;
RT   "Complete DNA sequences of two oka strain varicella-zoster virus genomes.";
RL   J. Virol. 82:11023-11044(2008).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=20844039; DOI=10.1128/jvi.00878-10;
RA   Kaufer B.B., Smejkal B., Osterrieder N.;
RT   "The varicella-zoster virus ORFS/L (ORF0) gene is required for efficient
RT   viral replication and contains an element involved in DNA cleavage.";
RL   J. Virol. 84:11661-11669(2010).
RN   [5]
RP   INTERACTION WITH HOST ITCH, DOMAIN, AND MUTAGENESIS OF TYR-47.
RX   PubMed=29535361; DOI=10.1038/s41598-018-22682-2;
RA   Koshizuka T., Kobayashi T., Ishioka K., Suzutani T.;
RT   "Herpesviruses possess conserved proteins for interaction with Nedd4 family
RT   ubiquitin E3 ligases.";
RL   Sci. Rep. 8:4447-4447(2018).
CC   -!- SUBUNIT: Interacts with host ITCH; this interaction probably mediates
CC       ITCH degradation. {ECO:0000305|PubMed:29535361}.
CC   -!- SUBCELLULAR LOCATION: Host Golgi apparatus membrane
CC       {ECO:0000269|PubMed:20844039}; Single-pass membrane protein
CC       {ECO:0000305}.
CC   -!- DOMAIN: Late-budding domains (L domains) are short sequence motifs
CC       essential for viral particle budding. They recruit proteins of the host
CC       ESCRT machinery (Endosomal Sorting Complex Required for Transport) or
CC       ESCRT-associated proteins. Contains one L domain: a PPXY motif which is
CC       involved in the interaction with ITCH, a member of the NEDD4 family.
CC       {ECO:0000269|PubMed:29535361}.
CC   -!- MISCELLANEOUS: The vaccine strains V-Oka, VariVax, and VarilRix harbor
CC       a mutation in the stop codon that extends the ORF by 92 aminoacids.
CC       {ECO:0000269|PubMed:20844039}.
CC   -!- SIMILARITY: Belongs to the varicellovirus ORF0 protein family.
CC       {ECO:0000305}.
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DR   EMBL; AF272392; AAG29812.1; -; Genomic_DNA.
DR   EMBL; AB097932; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AB097933; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; DQ008354; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; DQ008355; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; Q9E2H5; -.
DR   IntAct; Q9E2H5; 8.
DR   MINT; Q9E2H5; -.
DR   BRENDA; 1.3.1.21; 5301.
DR   Proteomes; UP000002603; Genome.
DR   Proteomes; UP000008504; Genome.
DR   Proteomes; UP000008505; Genome.
DR   Proteomes; UP000008506; Genome.
DR   Proteomes; UP000008507; Genome.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Host Golgi apparatus; Host membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..221
FT                   /note="Membrane protein 0"
FT                   /id="PRO_0000385142"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           44..47
FT                   /note="PPXY motif"
FT                   /evidence="ECO:0000269|PubMed:29535361"
FT   MUTAGEN         47
FT                   /note="Y->A: Complete loss of interaction with host ITCH."
FT                   /evidence="ECO:0000269|PubMed:29535361"
SQ   SEQUENCE   221 AA;  23825 MW;  75DFF52BDC099F32 CRC64;
     MATVHYSRRP GTPPVTLTSS PGMDDVATPI PYLPTYAEAV ADAPPPYRSR ESLVFSPPLF
     PHVENGTTQQ SYDCLDCAYD GIHRLQLAFL RIRKCCVPAF LILFGILTLT AVVVAIVAVF
     PEEPPNSTTR NYCPEGEGIY SRLQLVARVC TTKAIYVTKA NVAIWSTTPS TLHNLSICIF
     SCADAFLRDR GLGTSTSGIR TAGGLARTTS GDALFCISSV C