ID   ORF2P_CXB3N             Reviewed;          67 AA.
AC   P0DTB5;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2020, sequence version 1.
DT   02-DEC-2020, entry version 4.
DE   RecName: Full=ORF2p protein;
OS   Coxsackievirus B3 (strain Nancy).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Picornavirales; Picornaviridae; Enterovirus; Enterovirus B.
OX   NCBI_TaxID=103903;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2157045; DOI=10.1128/jvi.64.4.1573-1583.1990;
RA   Klump W.M., Bergmann I., Mueller B.C., Ameis D., Kandolf R.;
RT   "Complete nucleotide sequence of infectious Coxsackievirus B3 cDNA: two
RT   initial 5' uridine residues are regained during plus-strand RNA
RT   synthesis.";
RL   J. Virol. 64:1573-1583(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3027968; DOI=10.1016/0042-6822(87)90435-1;
RA   Lindberg A.M., Staalhandske P.O.K., Pettersson U.;
RT   "Genome of coxsackievirus B3.";
RL   Virology 156:50-63(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Gangaplara A., Massilamany C., Vu H., Pattnaik A.K., Reddy J.;
RL   Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=31492846; DOI=10.1038/s41467-019-12040-9;
RA   Guo H., Li Y., Liu G., Jiang Y., Shen S., Bi R., Huang H., Cheng T.,
RA   Wang C., Wei W.;
RT   "A second open reading frame in human enterovirus determines viral
RT   replication in intestinal epithelial cells.";
RL   Nat. Commun. 10:4066-4066(2019).
CC   -!- FUNCTION: Facilitates virus release from intestinal cells in vitro,
CC       possibly through the host autophagic pathway.
CC       {ECO:0000269|PubMed:31492846}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasmic vesicle membrane
CC       {ECO:0000250|UniProtKB:P0DTB4}; Single-pass membrane protein
CC       {ECO:0000255}.
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DR   EMBL; M33854; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; M16572; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; JX312064; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   Proteomes; UP000007760; Genome.
DR   Proteomes; UP000181225; Genome.
DR   Proteomes; UP000181606; Genome.
DR   GO; GO:0044162; C:host cell cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Host cytoplasmic vesicle; Host membrane; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..67
FT                   /note="ORF2p protein"
FT                   /id="PRO_0000449089"
FT   TRANSMEM        22..38
FT                   /evidence="ECO:0000255"
FT   REGION          13..18
FT                   /note="Important for viral replication in intestinal cells"
FT                   /evidence="ECO:0000269|PubMed:31492846"
FT   CONFLICT        7
FT                   /note="L -> S (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        26
FT                   /note="P -> L (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   67 AA;  7958 MW;  B5CA06ABDD15D4A7 CRC64;
     MVTIERLLPY SYWIGHPVTN RAIIYPFVGF IPLSLKEVKT LQFIVKLNTA KWELKYQRKR
     LGHMRPG