ID   ORF2P_HE71B             Reviewed;          75 AA.
AC   P0DTB4;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2020, sequence version 1.
DT   02-JUN-2021, entry version 4.
DE   RecName: Full=ORF2p protein;
OS   Human enterovirus 71 (strain USA/BrCr/1970) (EV71) (EV-71).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Picornavirales; Picornaviridae; Enterovirus; Enterovirus A.
OX   NCBI_TaxID=69153;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8837884; DOI=10.1016/0168-1702(95)00087-9;
RA   Brown B.A., Pallansch M.A.;
RT   "Complete nucleotide sequence of enterovirus 71 is distinct from
RT   poliovirus.";
RL   Virus Res. 39:195-206(1995).
RN   [2]
RP   IDENTIFICATION, FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF TYR-25;
RP   GLY-29 AND PRO-32.
RX   PubMed=31492846; DOI=10.1038/s41467-019-12040-9;
RA   Guo H., Li Y., Liu G., Jiang Y., Shen S., Bi R., Huang H., Cheng T.,
RA   Wang C., Wei W.;
RT   "A second open reading frame in human enterovirus determines viral
RT   replication in intestinal epithelial cells.";
RL   Nat. Commun. 10:4066-4066(2019).
CC   -!- FUNCTION: Facilitates virus release from intestinal cells in vitro,
CC       possibly through the host autophagic pathway.
CC       {ECO:0000269|PubMed:31492846}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasmic vesicle membrane
CC       {ECO:0000269|PubMed:31492846}; Single-pass membrane protein
CC       {ECO:0000255}.
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DR   EMBL; U22521; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   Proteomes; UP000007709; Genome.
DR   GO; GO:0044162; C:host cell cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Host cytoplasmic vesicle; Host membrane; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..75
FT                   /note="ORF2p protein"
FT                   /id="PRO_0000449069"
FT   TRANSMEM        23..45
FT                   /evidence="ECO:0000255"
FT   REGION          13..18
FT                   /note="Important for viral replication in intestinal cells"
FT                   /evidence="ECO:0000269|PubMed:31492846"
FT   REGION          53..75
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         25
FT                   /note="Y->A: Decreased virus yield; when associated with A-
FT                   29 and A-32."
FT                   /evidence="ECO:0000269|PubMed:31492846"
FT   MUTAGEN         29
FT                   /note="G->A: Decrased virus yield; when associated with A-
FT                   25 and A-32."
FT                   /evidence="ECO:0000269|PubMed:31492846"
FT   MUTAGEN         32
FT                   /note="P->A: Decrased virus yield; when associated with A-
FT                   25 and A-29."
FT                   /evidence="ECO:0000269|PubMed:31492846"
SQ   SEQUENCE   75 AA;  8712 MW;  0BE65458399BB7E7 CRC64;
     MVTIKELLPY SYWIGHPVSN RAIVYLFVGF TPLTLETLHT LNYIILLNTK RWAPRSPHSD
     PARMRIPTQP RKAPL