ORF2_USTMA
ID ORF2_USTMA Reviewed; 382 AA.
AC A0A0D1DNX6;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2015, sequence version 1.
DT 25-MAY-2022, entry version 23.
DE RecName: Full=Ustilagic acid biosynthesis cluster protein orf2 {ECO:0000303|PubMed:17850255};
GN Name=orf2 {ECO:0000303|PubMed:17850255}; ORFNames=UMAG_06465;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=521 / FGSC 9021;
RA Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RX PubMed=15932999; DOI=10.1128/aem.71.6.3033-3040.2005;
RA Hewald S., Josephs K., Boelker M.;
RT "Genetic analysis of biosurfactant production in Ustilago maydis.";
RL Appl. Environ. Microbiol. 71:3033-3040(2005).
RN [4]
RP FUNCTION, INDUCTION, AND PATHWAY.
RX PubMed=17850255; DOI=10.1111/j.1365-2958.2007.05941.x;
RA Teichmann B., Linne U., Hewald S., Marahiel M.A., Boelker M.;
RT "A biosynthetic gene cluster for a secreted cellobiose lipid with
RT antifungal activity from Ustilago maydis.";
RL Mol. Microbiol. 66:525-533(2007).
RN [5]
RP INDUCTION.
RX PubMed=20173069; DOI=10.1128/aem.02211-09;
RA Teichmann B., Liu L., Schink K.O., Boelker M.;
RT "Activation of the ustilagic acid biosynthesis gene cluster in Ustilago
RT maydis by the C2H2 zinc finger transcription factor Rua1.";
RL Appl. Environ. Microbiol. 76:2633-2640(2010).
CC -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC the glycolipid biosurfactant ustilagic acid (UA) (PubMed:15932999,
CC PubMed:17850255). UA is a secreted cellobiose glycolipid that is toxic
CC for many microorganisms and confers biocontrol activity to U.maydis
CC (PubMed:15932999, PubMed:17850255). UA consists of 15,16-
CC dihydroxypalmitic or 2,15,16-trihydroxypalmitic acid, which is O-
CC glycosidically linked to cellobiose at its terminal hydroxyl group
CC (PubMed:17850255). In addition, the cellobiose moiety is acetylated and
CC acylated with a short-chain hydroxy fatty acid (PubMed:17850255). UA
CC biosynthesis starts with omega-hydroxylation of palmitic acid catalyzed
CC by the cytochrome P450 monooxygenase cyp1 (PubMed:17850255). Terminal
CC hydroxylation of palmitic acid precedes subterminal hydroxylation
CC catalyzed by the cytochrome P450 monooxygenase cyp2 (PubMed:17850255).
CC Sequential glucosylation of the hydroxy fatty acid is probably
CC catalyzed by the glycosyltransferase ugt1 (Probable). The cellobiose
CC lipid is further decorated by acetylation of the proximal glucose
CC residue and by acylation with a short-chain beta-hydroxy fatty acid at
CC the distal glucose residue (Probable). The acyltransferase uat1 may be
CC a good candidate for catalyzing either acetylation or acylation of the
CC cellobiose lipid (Probable). The fatty acid synthase fas2 may be
CC involved in synthesis of the carbon backbone of the short-chain beta-
CC hydroxy fatty acid esterified to the cellobiose disaccharide
CC (Probable). The secreted UA consists of a mixture of both alpha-
CC hydroxylated and non-hydroxylated glycolipids; therefore, alpha-
CC hydroxylation of the long-chain fatty, catalyzed by the fatty acid
CC hydroxylase ahd1, occurs late in UA biosynthesis and may be the last
CC step before secretion (PubMed:17850255). {ECO:0000269|PubMed:15932999,
CC ECO:0000269|PubMed:17850255, ECO:0000305|PubMed:17850255}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000305|PubMed:17850255}.
CC -!- INDUCTION: Expression is strongly induced under conditions of nitrogen
CC starvation (PubMed:17850255). Expression is positively regulated by the
CC cluster-specific transcription factor rua1 that recognizes and binds to
CC the specific 5'-T/G-G/T-C-G-C-A-T-A/T-C/T-C/T-G/A-3' upstream
CC activating sequence found in all promoters of the UA biosynthesis genes
CC (PubMed:20173069). {ECO:0000269|PubMed:17850255,
CC ECO:0000269|PubMed:20173069}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CM003162; KIS65761.1; -; Genomic_DNA.
DR RefSeq; XP_011392732.1; XM_011394430.1.
DR AlphaFoldDB; A0A0D1DNX6; -.
DR SMR; A0A0D1DNX6; -.
DR EnsemblFungi; KIS65761; KIS65761; UMAG_06465.
DR GeneID; 23566046; -.
DR KEGG; uma:UMAG_06465; -.
DR VEuPathDB; FungiDB:UMAG_06465; -.
DR eggNOG; ENOG502TDMK; Eukaryota.
DR OrthoDB; 1306822at2759; -.
DR Proteomes; UP000000561; Chromosome 23.
DR Gene3D; 2.40.160.210; -; 1.
DR InterPro; IPR042171; Acyl-CoA_hotdog.
DR InterPro; IPR029069; HotDog_dom_sf.
DR SUPFAM; SSF54637; SSF54637; 1.
PE 2: Evidence at transcript level;
KW Reference proteome.
FT CHAIN 1..382
FT /note="Ustilagic acid biosynthesis cluster protein orf2"
FT /id="PRO_0000452766"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 382 AA; 43068 MW; 07449B7D413E656E CRC64;
MLQEAKVSTH TSNPLSQSVP QYGHSFDEAT RVERLDSAPE SLASVASTKL KDTTVWYRLA
IQSEWCSTPQ VPHGGFLASL LLSALLEHQQ SQQHPDPYTL SYDFLKVLSP GEAFISVTSL
GKVSGSFTSI AAELYQSRKE KVLLGVSVRG SFVNLSKQQG KSVLPLHYGA VSKLEEVPVP
HPRSWYLPPS QAMISRQLDL LVKLETQRRP FSDYFVRFHP DDVDTPQQED YSEEHASADL
QSSPHYCKGS RILPLRSSHS RRIDAKSIPM FGDFRRPAYE NVIKQDPCHP HDVPTRKFAF
PTLQYTIRFL AKVPHDTEWL HTQWRETVVD GRIISDVYIT TEQGAEPVAI CQLDSLMFDM
QWALSTMPSA EKSKKSPEAS QL