ORF3_GRVMC
ID ORF3_GRVMC Reviewed; 251 AA.
AC Q67684;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 02-JUN-2021, entry version 53.
DE RecName: Full=Long-distance movement protein;
DE AltName: Full=ORF3 protein;
GN Name=ORF3;
OS Groundnut rosette virus (strain MC1) (GRV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC Tolivirales; Tombusviridae; Calvusvirinae; Umbravirus.
OX NCBI_TaxID=1005060;
OH NCBI_TaxID=43365; Clitoria.
OH NCBI_TaxID=35936; Lablab purpureus (Hyacinth bean) (Dolichos lablab).
OH NCBI_TaxID=3879; Medicago sativa (Alfalfa).
OH NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH NCBI_TaxID=3917; Vigna unguiculata (Cowpea).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8811035; DOI=10.1099/0022-1317-77-9-2335;
RA Taliansky M.E., Robinson D.J., Murant A.F.;
RT "Complete nucleotide sequence and organization of the RNA genome of
RT groundnut rosette umbravirus.";
RL J. Gen. Virol. 77:2335-2345(1996).
RN [2]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND RNA-BINDING.
RX PubMed=12584328; DOI=10.1128/jvi.77.5.3031-3040.2003;
RA Taliansky M., Roberts I.M., Kalinina N., Ryabov E.V., Raj S.K.,
RA Robinson D.J., Oparka K.J.;
RT "An umbraviral protein, involved in long-distance RNA movement, binds viral
RT RNA and forms unique, protective ribonucleoprotein complexes.";
RL J. Virol. 77:3031-3040(2003).
RN [3]
RP SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, AND MUTAGENESIS OF
RP 108-ARG--ARG-122; LEU-149; LEU-152 AND LEU-153.
RX PubMed=17410203; DOI=10.1038/sj.emboj.7601674;
RA Kim S.H., Ryabov E.V., Kalinina N.O., Rakitina D.V., Gillespie T.,
RA MacFarlane S., Haupt S., Brown J.W., Taliansky M.;
RT "Cajal bodies and the nucleolus are required for a plant virus systemic
RT infection.";
RL EMBO J. 26:2169-2179(2007).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ARABIDOPSIS THALIANA FIB2,
RP AND MUTAGENESIS OF LEU-149.
RX PubMed=17576925; DOI=10.1073/pnas.0704632104;
RA Kim S.H., Macfarlane S., Kalinina N.O., Rakitina D.V., Ryabov E.V.,
RA Gillespie T., Haupt S., Brown J.W., Taliansky M.;
RT "Interaction of a plant virus-encoded protein with the major nucleolar
RT protein fibrillarin is required for systemic virus infection.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:11115-11120(2007).
CC -!- FUNCTION: Protects and provides long-distance movement to viral RNA.
CC Self associates and binds viral RNA and fibrillarin to form filamentous
CC ribonucleoproteins (RNPs) protected from RNase. ORF3 protein actually
CC fulfills functions that are usually provided by capsid protein, which
CC is absent from umbraviruses' genome. {ECO:0000269|PubMed:12584328,
CC ECO:0000269|PubMed:17576925}.
CC -!- SUBUNIT: Homooligomer. Interacts with host FIB2; this interaction, is
CC required for ORF3 protein transiting through host Cajal body and
CC nucleolus, relocalization of fibrillarin to the cytoplasm, and in
CC presence of viral RNA, leads to the formation of stable RNPs.
CC {ECO:0000269|PubMed:12584328, ECO:0000269|PubMed:17576925}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:12584328}.
CC Host nucleus, host nucleolus {ECO:0000269|PubMed:17410203,
CC ECO:0000269|PubMed:17576925}. Note=Passes through Cajal bodies to the
CC nucleolus and exits back into the cytoplasm (PubMed:17410203). This
CC journey is absolutely required for the formation of viral RNPs
CC particles (PubMed:17410203). In the cytoplasm, forms in large
CC cytoplasmic inclusions (PubMed:12584328).
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DR EMBL; Z69910; CAA93800.1; -; Genomic_RNA.
DR RefSeq; NP_619660.1; NC_003603.1.
DR DIP; DIP-60960N; -.
DR IntAct; Q67684; 1.
DR PRIDE; Q67684; -.
DR GeneID; 940202; -.
DR KEGG; vg:940202; -.
DR Proteomes; UP000008927; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR006902; Umbravirus_LDM.
DR Pfam; PF04817; Umbravirus_LDM; 1.
PE 1: Evidence at protein level;
KW Host cytoplasm; Host nucleus; Host-virus interaction; Reference proteome;
KW Ribonucleoprotein; RNA-binding; Transport; Viral movement protein.
FT CHAIN 1..251
FT /note="Long-distance movement protein"
FT /id="PRO_0000409934"
FT REGION 25..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 108..122
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 149..153
FT /note="Nuclear export signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 43..60
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 108..122
FT /note="RPRRRAGRSGGMDPR->APAAAAGASGGMDPA: Complete loss of
FT nuclear localization."
FT /evidence="ECO:0000269|PubMed:17410203"
FT MUTAGEN 149
FT /note="L->A: Complete loss of nucleolar localization and
FT nuclear export."
FT /evidence="ECO:0000269|PubMed:17410203,
FT ECO:0000269|PubMed:17576925"
FT MUTAGEN 152
FT /note="L->A: Increases nucleoplasmic accumulation."
FT /evidence="ECO:0000269|PubMed:17410203"
FT MUTAGEN 153
FT /note="L->A: Increases nucleoplasmic accumulation."
FT /evidence="ECO:0000269|PubMed:17410203"
SQ SEQUENCE 251 AA; 26684 MW; 0A822DC7A90D8124 CRC64;
MDTTPASRGQ WPVGIMSSVI NVFASGKSCN SGGAPRSSVR RGHRAGAARD KSRGFDAPPR
RPKGGVHPAT TSKDPNKDIR GPPAPTPHKK YRGPAIPGEG RSGVHTTRPR RRAGRSGGMD
PRQLVAQPQQ RWAKTEIPTE RRAEIDGLLP SLLNTLDGQI QGDAALLRYC VGAIKRELRR
RWESVQPAHH VAASSGKPSP QLFNEAAQNA EDLSGDGKGC AGQSVQQEVL HSGSGVPPVC
ADCGKPAANK W
