ID   ORF3_HEVCT              Reviewed;         112 AA.
AC   Q9IVZ7;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Protein ORF3;
DE            Short=pORF3;
GN   ORFNames=ORF3;
OS   Hepatitis E virus genotype 4 (isolate Human/China/T1) (HEV-4) (Hepatitis E
OS   virus genotype 4 (isolate Human/China/Ct1)).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Hepelivirales; Hepeviridae; Orthohepevirus; Hepatitis E virus.
OX   NCBI_TaxID=509627;
OH   NCBI_TaxID=69079; Bandicota bengalensis (lesser bandicoot rat).
OH   NCBI_TaxID=9481; Callithrix.
OH   NCBI_TaxID=9536; Cercopithecus hamlyni (Owl-faced monkey) (Hamlyn's monkey).
OH   NCBI_TaxID=9534; Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops).
OH   NCBI_TaxID=9031; Gallus gallus (Chicken).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9539; Macaca (macaques).
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
OH   NCBI_TaxID=9598; Pan troglodytes (Chimpanzee).
OH   NCBI_TaxID=9520; Saimiri (squirrel monkeys).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=10859372; DOI=10.1099/0022-1317-81-7-1675;
RA   Wang Y., Zhang H., Ling R., Li H., Harrison T.J.;
RT   "The complete sequence of hepatitis E virus genotype 4 reveals an
RT   alternative strategy for translation of open reading frames 2 and 3.";
RL   J. Gen. Virol. 81:1675-1686(2000).
CC   -!- FUNCTION: May act as a viral regulatory protein involved in the
CC       modulation of mitogenic signaling pathways. May be involved in virion
CC       morphogenesis and viral pathogenesis. Expedites the processing and
CC       secretion of AMBP from the hepatocyte (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. Interacts with host SRC, HCK, FYN, PIK3R3 and GRB2
CC       (via SH3 domain); binding does not activate the kinases. Interacts with
CC       host AMBP/bikunin and AMBP/alpha-1-microglobulin peptides. Interacts
CC       with host HPX/hemopexin (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}. Note=The N-terminal
CC       region seems to associate with the cytoskeleton probably via one of its
CC       hydrophobic regions. {ECO:0000250}.
CC   -!- PTM: Not glycosylated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the hepevirus ORF3 protein family.
CC       {ECO:0000305}.
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DR   EMBL; AJ272108; CAB83211.1; -; Genomic_RNA.
DR   PRIDE; Q9IVZ7; -.
DR   Proteomes; UP000007242; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR003384; HEV_Orf2.
DR   Pfam; PF02444; HEV_ORF1; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host-virus interaction.
FT   CHAIN           1..112
FT                   /note="Protein ORF3"
FT                   /id="PRO_0000334541"
FT   REGION          7..23
FT                   /note="Hydrophobic"
FT   REGION          28..66
FT                   /note="Interaction with host HPX"
FT                   /evidence="ECO:0000250"
FT   REGION          40..60
FT                   /note="Hydrophobic"
FT   REGION          70..112
FT                   /note="Homodimerization, and interaction with host
FT                   AMBP/bikunin"
FT                   /evidence="ECO:0000250"
FT   REGION          89..112
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          93..102
FT                   /note="Interaction with host SRC, HCK, FYN, PIK3R3 and
FT                   GRB2"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   112 AA;  11565 MW;  975A55969DE7F5A6 CRC64;
     MEMPPCALGL FCFCSSCFCL CCPRHRPVSR LAVAAGKRGA AVVSGVTGLI LSPSPSPIFI
     QPTPSHLTFQ PPPGLELALG SQSVHSAPLG VTSPSAPPLP PVVDLPQLGL RR