ORF3_HEVMG
ID ORF3_HEVMG Reviewed; 113 AA.
AC Q6J8G1; O36612;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 23-FEB-2022, entry version 51.
DE RecName: Full=Protein ORF3;
DE Short=pORF3;
GN ORFNames=ORF3;
OS Hepatitis E virus genotype 3 (isolate Swine/United States/swUS1) (HEV-3)
OS (Hepatitis E virus genotype 3 (isolate Swine/United States/Meng)).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Hepelivirales; Hepeviridae; Orthohepevirus; Hepatitis E virus.
OX NCBI_TaxID=512345;
OH NCBI_TaxID=69079; Bandicota bengalensis (lesser bandicoot rat).
OH NCBI_TaxID=9481; Callithrix.
OH NCBI_TaxID=9536; Cercopithecus hamlyni (Owl-faced monkey) (Hamlyn's monkey).
OH NCBI_TaxID=9534; Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops).
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=9539; Macaca (macaques).
OH NCBI_TaxID=10090; Mus musculus (Mouse).
OH NCBI_TaxID=9598; Pan troglodytes (Chimpanzee).
OH NCBI_TaxID=9520; Saimiri (squirrel monkeys).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=9275216; DOI=10.1073/pnas.94.18.9860;
RA Meng X.J., Purcell R.H., Halbur P.G., Lehman J.R., Webb D.M., Tsareva T.S.,
RA Haynes J.S., Thacker B.J., Emerson S.U.;
RT "A novel virus in swine is closely related to the human hepatitis E
RT virus.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:9860-9865(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=9811705; DOI=10.1128/jvi.72.12.9714-9721.1998;
RA Meng X.J., Halbur P.G., Shapiro M.S., Govindarajan S., Bruna J.D.,
RA Mushahwar I.K., Purcell R.H., Emerson S.U.;
RT "Genetic and experimental evidence for cross-species infection by swine
RT hepatitis E virus.";
RL J. Virol. 72:9714-9721(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate pSHEV-1, Isolate pSHEV-2, and Isolate pSHEV-3;
RX PubMed=15650181; DOI=10.1128/jvi.79.3.1552-1558.2005;
RA Huang Y.W., Haqshenas G., Kasorndorkbua C., Halbur P.G., Emerson S.U.,
RA Meng X.J.;
RT "Capped RNA transcripts of full-length cDNA clones of swine hepatitis E
RT virus are replication competent when transfected into Huh7 cells and
RT infectious when intrahepatically inoculated into pigs.";
RL J. Virol. 79:1552-1558(2005).
RN [4]
RP IDENTIFICATION OF START CODON.
RX PubMed=17202216; DOI=10.1128/jvi.02259-06;
RA Huang Y.W., Opriessnig T., Halbur P.G., Meng X.J.;
RT "Initiation at the third in-frame AUG codon of open reading frame 3 of the
RT hepatitis E virus is essential for viral infectivity in vivo.";
RL J. Virol. 81:3018-3026(2007).
CC -!- FUNCTION: May act as a viral regulatory protein involved in the
CC modulation of mitogenic signaling pathways. May be involved in virion
CC morphogenesis and viral pathogenesis. Expedites the processing and
CC secretion of AMBP from the hepatocyte (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Interacts with host SRC, HCK, FYN, PIK3R3 and GRB2
CC (via SH3 domain); binding does not activate the kinases. Interacts with
CC host AMBP/bikunin and AMBP/alpha-1-microglobulin peptides. Interacts
CC with host HPX/hemopexin. The phosphorylated form interacts with the
CC unglycosylated capsid protein (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}. Note=The N-terminal
CC region seems to associate with the cytoskeleton probably via one of its
CC hydrophobic regions. {ECO:0000250}.
CC -!- PTM: Not glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the hepevirus ORF3 protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC97209.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAT40996.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAT40999.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAT41002.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF082843; AAC97209.1; ALT_INIT; Genomic_RNA.
DR EMBL; AY575857; AAT40996.1; ALT_INIT; Genomic_RNA.
DR EMBL; AY575858; AAT40999.1; ALT_INIT; Genomic_RNA.
DR EMBL; AY575859; AAT41002.1; ALT_INIT; Genomic_RNA.
DR Proteomes; UP000001028; Genome.
DR Proteomes; UP000008858; Genome.
DR Proteomes; UP000008859; Genome.
DR Proteomes; UP000008989; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR InterPro; IPR003384; HEV_Orf2.
DR Pfam; PF02444; HEV_ORF1; 1.
PE 3: Inferred from homology;
KW Host cytoplasm; Host-virus interaction; Phosphoprotein.
FT CHAIN 1..113
FT /note="Protein ORF3"
FT /id="PRO_5000055032"
FT REGION 1..21
FT /note="Hydrophobic"
FT REGION 27..67
FT /note="Interaction with host HPX"
FT /evidence="ECO:0000250"
FT REGION 32..52
FT /note="Hydrophobic"
FT REGION 47..71
FT /note="Interaction with the capsid protein"
FT /evidence="ECO:0000250"
FT REGION 71..113
FT /note="Homodimerization, and interaction with host
FT AMBP/bikunin"
FT /evidence="ECO:0000250"
FT REGION 89..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 94..103
FT /note="Interaction with host SRC, HCK, FYN, PIK3R3 and
FT GRB2"
FT /evidence="ECO:0000250"
FT COMPBIAS 91..105
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 70
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250"
FT CONFLICT 78
FT /note="L -> F (in Ref. 1 and 2; AAC97209)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 113 AA; 11452 MW; 73595DF8733D4250 CRC64;
MGSPCALGLF CCCSSCFCLC CPRHRPASRL AAVVGGATAV PAVVSGVTGL ILSPSPSPIF
IQPTPSLPMS FHNPGLELAL DSRPAPLAPL GVTSPSAPPL PPVVDLPQLG LRR
