ID   ORF3_HEVUS              Reviewed;         113 AA.
AC   Q9YLR0;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   29-SEP-2021, entry version 63.
DE   RecName: Full=Protein ORF3;
DE            Short=pORF3;
GN   ORFNames=ORF3;
OS   Hepatitis E virus genotype 3 (isolate Human/United States/US2) (HEV-3).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Hepelivirales; Hepeviridae; Orthohepevirus; Hepatitis E virus.
OX   NCBI_TaxID=509615;
OH   NCBI_TaxID=69079; Bandicota bengalensis (lesser bandicoot rat).
OH   NCBI_TaxID=9481; Callithrix.
OH   NCBI_TaxID=9536; Cercopithecus hamlyni (Owl-faced monkey) (Hamlyn's monkey).
OH   NCBI_TaxID=9534; Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9539; Macaca (macaques).
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
OH   NCBI_TaxID=9598; Pan troglodytes (Chimpanzee).
OH   NCBI_TaxID=9520; Saimiri (squirrel monkeys).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10092008; DOI=10.1099/0022-1317-80-3-681;
RA   Erker J.C., Desai S.M., Schlauder G.G., Dawson G.J., Mushahwar I.K.;
RT   "A hepatitis E virus variant from the United States: molecular
RT   characterization and transmission in cynomolgus macaques.";
RL   J. Gen. Virol. 80:681-690(1999).
CC   -!- FUNCTION: May act as a viral regulatory protein involved in the
CC       modulation of mitogenic signaling pathways. May be involved in virion
CC       morphogenesis and viral pathogenesis. Expedites the processing and
CC       secretion of AMBP from the hepatocyte (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. Interacts with host SRC, HCK, FYN, PIK3R3 and GRB2
CC       (via SH3 domain); binding does not activate the kinases. Interacts with
CC       host AMBP/bikunin and AMBP/alpha-1-microglobulin peptides. Interacts
CC       with host HPX/hemopexin. The phosphorylated form interacts with the
CC       unglycosylated capsid protein (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}. Note=The N-terminal
CC       region seems to associate with the cytoskeleton probably via one of its
CC       hydrophobic regions. {ECO:0000250}.
CC   -!- PTM: Not glycosylated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the hepevirus ORF3 protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD15817.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF060669; AAD15817.1; ALT_INIT; Genomic_DNA.
DR   SwissPalm; Q9YLR0; -.
DR   Proteomes; UP000007247; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR003384; HEV_Orf2.
DR   Pfam; PF02444; HEV_ORF1; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host-virus interaction; Phosphoprotein.
FT   CHAIN           1..113
FT                   /note="Protein ORF3"
FT                   /id="PRO_0000334540"
FT   REGION          1..21
FT                   /note="Hydrophobic"
FT   REGION          27..67
FT                   /note="Interaction with host HPX"
FT                   /evidence="ECO:0000250"
FT   REGION          32..52
FT                   /note="Hydrophobic"
FT   REGION          47..71
FT                   /note="Interaction with the capsid protein"
FT                   /evidence="ECO:0000250"
FT   REGION          71..113
FT                   /note="Homodimerization, and interaction with host
FT                   AMBP/bikunin"
FT                   /evidence="ECO:0000250"
FT   REGION          87..109
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          94..103
FT                   /note="Interaction with host SRC, HCK, FYN, PIK3R3 and
FT                   GRB2"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        91..105
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         70
FT                   /note="Phosphoserine; by host"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   113 AA;  11446 MW;  22E48C61623C489A CRC64;
     MGSPCALGLF CCCSSCFCLC CPRHRPASRL AAVVGGAAAV PAVVSGVTGL ILSPSPSPIF
     IQPTPSPPMS FHNPGLELAL DSRPAPLXPL GVTSPSAPPL PPVVDLPQLG LRR