ID   ORF3_PCV2               Reviewed;         104 AA.
AC   O56124; Q9YR18;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   23-FEB-2022, entry version 55.
DE   RecName: Full=Protein ORF3;
GN   ORFNames=ORF3;
OS   Porcine circovirus 2 (PCV2).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes;
OC   Cirlivirales; Circoviridae; Circovirus.
OX   NCBI_TaxID=85708;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9573301; DOI=10.1128/jvi.72.6.5262-5267.1998;
RA   Hamel A.L., Lin L.L., Nayar G.P.;
RT   "Nucleotide sequence of porcine circovirus associated with postweaning
RT   multisystemic wasting syndrome in pigs.";
RL   J. Virol. 72:5262-5267(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9747726; DOI=10.1099/0022-1317-79-9-2171;
RA   Meehan B.M., McNeilly F., Todd D., Kennedy S., Jewhurst V.A., Ellis J.A.,
RA   Hassard L.E., Clark E.G., Haines D.M., Allan G.M.;
RT   "Characterization of novel circovirus DNAs associated with wasting
RT   syndromes in pigs.";
RL   J. Gen. Virol. 79:2171-2179(1998).
RN   [3]
RP   FUNCTION.
RX   PubMed=15956572; DOI=10.1128/jvi.79.13.8262-8274.2005;
RA   Liu J., Chen I., Kwang J.;
RT   "Characterization of a previously unidentified viral protein in porcine
RT   circovirus type 2-infected cells and its role in virus-induced apoptosis.";
RL   J. Virol. 79:8262-8274(2005).
RN   [4]
RP   REVIEW.
RX   PubMed=25011695; DOI=10.1007/s11262-014-1099-z;
RA   Lv Q.Z., Guo K.K., Zhang Y.M.;
RT   "Current understanding of genomic DNA of porcine circovirus type 2.";
RL   Virus Genes 49:1-10(2014).
RN   [5]
RP   FUNCTION, AND INTERACTION WITH HOST RGS16.
RX   PubMed=25575706; DOI=10.1099/vir.0.000046;
RA   Choi C.Y., Rho S.B., Kim H.S., Han J., Bae J., Lee S.J., Jung W.W.,
RA   Chun T.;
RT   "The ORF3 protein of porcine circovirus type 2 promotes secretion of IL-6
RT   and IL-8 in porcine epithelial cells by facilitating proteasomal
RT   degradation of regulator of G protein signalling 16 through physical
RT   interaction.";
RL   J. Gen. Virol. 96:1098-1108(2015).
RN   [6]
RP   SUBCELLULAR LOCATION.
RX   PubMed=26854343; DOI=10.1016/j.vetmic.2016.01.006;
RA   Gu J., Wang L., Jin Y., Lin C., Wang H., Zhou N., Xing G., Liao M.,
RA   Zhou J.;
RT   "Characterization of specific antigenic epitopes and the nuclear export
RT   signal of the Porcine circovirus 2 ORF3 protein.";
RL   Vet. Microbiol. 184:40-50(2016).
CC   -!- FUNCTION: Plays a role in modulating host cell signaling by binding to
CC       and degrading host RGS16 (PubMed:25575706). Not necessary for virus
CC       replication (PubMed:15956572). {ECO:0000269|PubMed:15956572,
CC       ECO:0000269|PubMed:25575706}.
CC   -!- SUBUNIT: Interacts with host RGS16. {ECO:0000269|PubMed:25575706}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:26854343}.
CC       Host nucleus {ECO:0000269|PubMed:26854343}. Note=Expressed in cytoplasm
CC       in early stages of PCV2 infection and then accumulated in nucleus over
CC       time. {ECO:0000269|PubMed:26854343}.
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DR   EMBL; AF027217; AAC59464.1; -; Genomic_DNA.
DR   EMBL; AF055391; AAC35300.1; -; Genomic_DNA.
DR   EMBL; AF055392; AAC35311.1; -; Genomic_DNA.
DR   Proteomes; UP000115010; Genome.
DR   Proteomes; UP000150239; Genome.
DR   Proteomes; UP000161967; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008500; Circovirus_Orf3.
DR   Pfam; PF05614; DUF782; 1.
PE   1: Evidence at protein level;
KW   Apoptosis; Host cytoplasm; Host nucleus; Host-virus interaction.
FT   CHAIN           1..104
FT                   /note="Protein ORF3"
FT                   /id="PRO_0000133087"
FT   REGION          78..104
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           1..35
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000269|PubMed:26854343"
SQ   SEQUENCE   104 AA;  11879 MW;  DD83D288A824E869 CRC64;
     MVTIPPLVSR WFPVCGFRVC KISSPFAFTT PRWPHNDVYI GLPITLLHFP AHFQKFSQPA
     EISDKRYRVL LCNGHQTPAL QQGTHSSRQV TPLSLRSRSS TFNK