ID   ORF4B_BCHK5             Reviewed;         256 AA.
AC   A3EXD3;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Non-structural protein ORF4b;
DE            Short=ORF4b;
GN   Name=ORF4b;
OS   Bat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Merbecovirus.
OX   NCBI_TaxID=694008;
OH   NCBI_TaxID=105295; Pipistrellus abramus (Japanese pipistrelle) (Pipistrellus javanicus abramus).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HKU5-1;
RX   PubMed=17121802; DOI=10.1128/jvi.02182-06;
RA   Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L.,
RA   Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H.,
RA   Zheng B.-J., Yuen K.-Y.;
RT   "Comparative analysis of twelve genomes of three novel group 2c and group
RT   2d coronaviruses reveals unique group and subgroup features.";
RL   J. Virol. 81:1574-1585(2007).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=24443473; DOI=10.1099/vir.0.062059-0;
RA   Matthews K.L., Coleman C.M., van der Meer Y., Snijder E.J., Frieman M.B.;
RT   "The ORF4b-encoded accessory proteins of Middle East respiratory syndrome
RT   coronavirus and two related bat coronaviruses localize to the nucleus and
RT   inhibit innate immune signalling.";
RL   J. Gen. Virol. 95:874-882(2014).
CC   -!- FUNCTION: Plays a role in the inhibition of host innate immunity by
CC       inhibiting the interaction between host IKBKE and MAVS. In turn, this
CC       inhibition prevents the production of host interferon beta.
CC       Additionally, may also interfere with host antiviral response within
CC       the nucleus. {ECO:0000269|PubMed:24443473}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:24443473}. Host
CC       nucleus, host nucleolus {ECO:0000250|UniProtKB:K9N643}. Host cytoplasm
CC       {ECO:0000250|UniProtKB:K9N643}. Note=Mainly localized in the host
CC       nucleus. {ECO:0000250|UniProtKB:K9N643}.
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DR   EMBL; EF065509; ABN10878.1; -; Genomic_RNA.
DR   RefSeq; YP_001039965.1; NC_009020.1.
DR   PDB; 7RG6; X-ray; 2.10 A; C/D=29-48.
DR   PDBsum; 7RG6; -.
DR   SMR; A3EXD3; -.
DR   GeneID; 4836006; -.
DR   KEGG; vg:4836006; -.
DR   Proteomes; UP000007451; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
DR   GO; GO:0039501; P:suppression by virus of host type I interferon production; IDA:UniProtKB.
DR   GO; GO:0039724; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity; IEA:UniProtKB-KW.
DR   CDD; cd21653; ORF4b_HKU5-CoV; 1.
DR   InterPro; IPR044318; ORF4b_HKU5-CoV.
PE   1: Evidence at protein level;
KW   3D-structure; Host cytoplasm; Host nucleus; Host-virus interaction;
KW   Inhibition of host IKBKE by virus;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host RLR pathway by virus; Reference proteome;
KW   Viral immunoevasion.
FT   CHAIN           1..256
FT                   /note="Non-structural protein ORF4b"
FT                   /id="PRO_0000290269"
SQ   SEQUENCE   256 AA;  29427 MW;  A3E7E7022CE7962F CRC64;
     MDDSMDLDLD CVIAQPSSTI VMMPLSPIST RKRRRHPMNK RRYAKRRFTP VEPNDIIMCD
     KPTHCIRLVF DQSLRWVHFD GIKNILTDYD VIFNPDLHVT VALVCAGNGV TFSDLTPLTF
     ILADMLLEFN GIFTLGQTLV IGAREYHWLP QELKTNVGKA IPQAKEWLVD HGYNVYHTGL
     PTHMSLAKLH SLDFVQQSYV GSKFFIKHSH TTEYAMPVCL QVIAIDGEKV DGRSKPLFQY
     PIHNHYRHYR ACFPGR