ID   ORF5A_PRRSR             Reviewed;          51 AA.
AC   P0DJZ5;
DT   11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT   11-NOV-2015, sequence version 1.
DT   23-FEB-2022, entry version 11.
DE   RecName: Full=Structural protein ORF5a;
OS   Porcine reproductive and respiratory syndrome virus (strain VR-2332)
OS   (PRRSV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Arnidovirineae; Arteriviridae; unclassified Arteriviridae.
OX   NCBI_TaxID=300559;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=9847330; DOI=10.1128/jvi.73.1.270-280.1999;
RA   Nelsen C.J., Murtaugh M.P., Faaberg K.S.;
RT   "Porcine reproductive and respiratory syndrome virus comparison: divergent
RT   evolution on two continents.";
RL   J. Virol. 73:270-280(1999).
RN   [2]
RP   SEQUENCE REVISION.
RA   Murtaugh M.P., Faaberg K.S., Nelsen C.J.;
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Infectious clone VR-2332;
RX   PubMed=12610145; DOI=10.1128/jvi.77.6.3702-3711.2003;
RA   Nielsen H.S., Liu G., Nielsen J., Oleksiewicz M.B., Botner A.,
RA   Storgaard T., Faaberg K.S.;
RT   "Generation of an infectious clone of VR-2332, a highly virulent North
RT   American-type isolate of porcine reproductive and respiratory syndrome
RT   virus.";
RL   J. Virol. 77:3702-3711(2003).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=21307223; DOI=10.1099/vir.0.029264-0;
RA   Firth A.E., Zevenhoven-Dobbe J.C., Wills N.M., Go Y.Y., Balasuriya U.B.,
RA   Atkins J.F., Snijder E.J., Posthuma C.C.;
RT   "Discovery of a small arterivirus gene that overlaps the GP5 coding
RT   sequence and is important for virus production.";
RL   J. Gen. Virol. 92:1097-1106(2011).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   PubMed=21307222; DOI=10.1099/vir.0.030213-0;
RA   Johnson C.R., Griggs T.F., Gnanandarajah J., Murtaugh M.P.;
RT   "Novel structural protein in porcine reproductive and respiratory syndrome
RT   virus encoded by an alternative ORF5 present in all arteriviruses.";
RL   J. Gen. Virol. 92:1107-1116(2011).
RN   [6]
RP   FUNCTION, AND INTERACTION WITH GP2B AND GP4.
RX   PubMed=25499299; DOI=10.1016/j.virusres.2014.12.004;
RA   Sun L., Zhou Y., Liu R., Li Y., Gao F., Wang X., Fan H., Yuan S., Wei Z.,
RA   Tong G.;
RT   "Cysteine residues of the porcine reproductive and respiratory syndrome
RT   virus ORF5a protein are not essential for virus viability.";
RL   Virus Res. 197:17-25(2015).
CC   -!- FUNCTION: Minor virion component that plays an essential role in virus
CC       infectivity. {ECO:0000269|PubMed:25499299}.
CC   -!- SUBUNIT: Interacts with GP2b and GP4. {ECO:0000269|PubMed:25499299}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:21307222}. Host cell
CC       membrane {ECO:0000269|PubMed:21307222}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=ORF5a; Synonyms=Protein ORF5a;
CC         IsoId=P0DJZ5-1; Sequence=Displayed;
CC       Name=GP5; Synonyms=Glycoprotein 5;
CC         IsoId=P0DJZ5-2; Sequence=Not described;
CC   -!- SIMILARITY: Belongs to the arteriviridae ORF5a protein family.
CC       {ECO:0000305}.
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DR   EMBL; U87392; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY150564; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   SMR; P0DJZ5; -.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Alternative initiation; Host cell membrane; Host membrane; Membrane;
KW   Transmembrane; Transmembrane helix; Virion.
FT   CHAIN           1..51
FT                   /note="Structural protein ORF5a"
FT                   /id="PRO_0000434788"
FT   TRANSMEM        11..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   51 AA;  5926 MW;  F56749250221AE49 CRC64;
     MFKYVGEMLD RGLLLAIAFF VVYRAVLFCC ARQRQQRQQL PSTADLQLDA M