ID   ORG2_ARATH              Reviewed;         253 AA.
AC   Q9M1K1;
DT   16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Transcription factor ORG2;
DE   AltName: Full=Basic helix-loop-helix protein 38;
DE            Short=AtbHLH38;
DE            Short=bHLH 38;
DE   AltName: Full=OBP3-responsive gene 2;
DE   AltName: Full=Transcription factor EN 8;
DE   AltName: Full=bHLH transcription factor bHLH038;
GN   Name=ORG2; Synonyms=BHLH38, EN8; OrderedLocusNames=At3g56970;
GN   ORFNames=F24I3.50;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, AND
RP   NOMENCLATURE.
RC   STRAIN=cv. Columbia;
RX   PubMed=12679534; DOI=10.1093/molbev/msg088;
RA   Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT   "The basic helix-loop-helix transcription factor family in plants: a
RT   genome-wide study of protein structure and functional diversity.";
RL   Mol. Biol. Evol. 20:735-747(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   GENE FAMILY.
RX   PubMed=12897250; DOI=10.1105/tpc.013839;
RA   Toledo-Ortiz G., Huq E., Quail P.H.;
RT   "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL   Plant Cell 15:1749-1770(2003).
RN   [7]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=14600211; DOI=10.1105/tpc.151140;
RA   Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA   Jakoby M., Werber M., Weisshaar B.;
RT   "Update on the basic helix-loop-helix transcription factor gene family in
RT   Arabidopsis thaliana.";
RL   Plant Cell 15:2497-2502(2003).
RN   [8]
RP   INDUCTION BY OBP3; JASMONIC ACID AND SALICYLIC ACID.
RX   PubMed=12887587; DOI=10.1046/j.1365-313x.2003.01812.x;
RA   Kang H.-G., Foley R.C., Onate-Sanchez L., Lin C., Singh K.B.;
RT   "Target genes for OBP3, a Dof transcription factor, include novel basic
RT   helix-loop-helix domain proteins inducible by salicylic acid.";
RL   Plant J. 35:362-372(2003).
RN   [9]
RP   INDUCTION.
RX   PubMed=17516080; DOI=10.1007/s00425-007-0535-x;
RA   Wang H.-Y., Klatte M., Jakoby M., Baeumlein H., Weisshaar B., Bauer P.;
RT   "Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes
RT   in Arabidopsis thaliana.";
RL   Planta 226:897-908(2007).
CC   -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC   -!- INTERACTION:
CC       Q9M1K1; Q0V7X4: FIT; NbExp=5; IntAct=EBI-1640553, EBI-1640543;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- TISSUE SPECIFICITY: Roots. {ECO:0000269|PubMed:12679534}.
CC   -!- INDUCTION: Induced by OBP3, auxin and salicylic acid (SA). Repressed by
CC       jasmonic acid (JA), UV LIGHT, and heat treatments. Up regulated by iron
CC       deficiency in roots and leaves, as well as by nickel, high zinc or high
CC       copper treatments. Repressed by high iron, low copper and low zinc
CC       treatments. {ECO:0000269|PubMed:12679534, ECO:0000269|PubMed:12887587,
CC       ECO:0000269|PubMed:17516080}.
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DR   EMBL; AF488576; AAM10940.1; -; mRNA.
DR   EMBL; AL138655; CAB72167.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE79594.1; -; Genomic_DNA.
DR   EMBL; AK221542; BAD94899.1; -; mRNA.
DR   EMBL; BT026419; ABH04526.1; -; mRNA.
DR   PIR; T47757; T47757.
DR   RefSeq; NP_191256.1; NM_115556.4.
DR   AlphaFoldDB; Q9M1K1; -.
DR   SMR; Q9M1K1; -.
DR   BioGRID; 10180; 8.
DR   IntAct; Q9M1K1; 8.
DR   STRING; 3702.AT3G56970.1; -.
DR   PaxDb; Q9M1K1; -.
DR   PRIDE; Q9M1K1; -.
DR   DNASU; 824864; -.
DR   EnsemblPlants; AT3G56970.1; AT3G56970.1; AT3G56970.
DR   GeneID; 824864; -.
DR   Gramene; AT3G56970.1; AT3G56970.1; AT3G56970.
DR   KEGG; ath:AT3G56970; -.
DR   Araport; AT3G56970; -.
DR   TAIR; locus:2080600; AT3G56970.
DR   eggNOG; ENOG502RXMR; Eukaryota.
DR   HOGENOM; CLU_089779_1_1_1; -.
DR   InParanoid; Q9M1K1; -.
DR   OMA; AIQISMR; -.
DR   OrthoDB; 1275266at2759; -.
DR   PhylomeDB; Q9M1K1; -.
DR   PRO; PR:Q9M1K1; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9M1K1; baseline and differential.
DR   GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0010106; P:cellular response to iron ion starvation; IEP:TAIR.
DR   GO; GO:0055072; P:iron ion homeostasis; IGI:TAIR.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR015660; MASH1/Ascl1a-like.
DR   PANTHER; PTHR13935; PTHR13935; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..253
FT                   /note="Transcription factor ORG2"
FT                   /id="PRO_0000358850"
FT   DOMAIN          71..123
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ   SEQUENCE   253 AA;  28714 MW;  9DE64B574869394A CRC64;
     MCALVPSFFT NFGWPSTNQY ESYYGAGDNL NNGTFLELTV PQTYEVTHHQ NSLGVSVSSE
     GNEIDNNPVV VKKLNHNASE RDRRKKINTL FSSLRSCLPA SDQSKKLSIP ETVSKSLKYI
     PELQQQVKRL IQKKEEILVR VSGQRDFELY DKQQPKAVAS YLSTVSATRL GDNEVMVQVS
     SSKIHNFSIS NVLGGIEEDG FVLVDVSSSR SQGERLFYTL HLQVENMDDY KINCEELSER
     MLYLYEKCEN SFN