ORL2A_ARATH
ID ORL2A_ARATH Reviewed; 269 AA.
AC P0DI08; Q9C5W1; Q9LDU9;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2012, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Putative 12-oxophytodienoate reductase-like protein 2A;
DE EC=1.3.1.-;
GN OrderedLocusNames=At1g17990; ORFNames=F2H15.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Putative oxophytodienoate reductase that may be involved in
CC the biosynthesis or metabolism of oxylipin signaling molecules.
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=P0DI08-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF97278.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC034106; AAF97278.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29660.1; -; Genomic_DNA.
DR EMBL; AK118160; BAC42784.1; -; mRNA.
DR EMBL; AF344314; AAK06865.1; -; mRNA.
DR EMBL; AY063976; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; F86315; F86315.
DR RefSeq; NP_173241.2; NM_101664.5. [P0DI08-1]
DR RefSeq; NP_849683.1; NM_179352.5. [P0DI08-1]
DR AlphaFoldDB; P0DI08; -.
DR SMR; P0DI08; -.
DR STRING; 3702.AT1G17990.1; -.
DR iPTMnet; P0DI08; -.
DR PaxDb; P0DI08; -.
DR PRIDE; P0DI08; -.
DR EnsemblPlants; AT1G17990.1; AT1G17990.1; AT1G17990. [P0DI08-1]
DR EnsemblPlants; AT1G18020.1; AT1G18020.1; AT1G18020. [P0DI08-1]
DR GeneID; 838379; -.
DR GeneID; 838382; -.
DR Gramene; AT1G17990.1; AT1G17990.1; AT1G17990. [P0DI08-1]
DR Gramene; AT1G18020.1; AT1G18020.1; AT1G18020. [P0DI08-1]
DR KEGG; ath:AT1G17990; -.
DR KEGG; ath:AT1G18020; -.
DR Araport; AT1G17990; -.
DR TAIR; locus:2030948; AT1G17990.
DR eggNOG; KOG0134; Eukaryota.
DR HOGENOM; CLU_012153_0_1_1; -.
DR InParanoid; P0DI08; -.
DR OMA; SWRIRIC; -.
DR PhylomeDB; P0DI08; -.
DR PRO; PR:P0DI08; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P0DI08; baseline and differential.
DR Genevisible; P0DI08; AT.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR001155; OxRdtase_FMN_N.
DR InterPro; IPR045247; Oye-like.
DR PANTHER; PTHR22893; PTHR22893; 1.
DR Pfam; PF00724; Oxidored_FMN; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Flavoprotein; FMN; NADP; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..269
FT /note="Putative 12-oxophytodienoate reductase-like protein
FT 2A"
FT /id="PRO_0000194487"
FT ACT_SITE 180
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 28..30
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250"
FT BINDING 103
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250"
FT BINDING 175..178
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 227
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250"
SQ SEQUENCE 269 AA; 29973 MW; 2E88012500F7515F CRC64;
METKQSIPLL MPYKMGPFNL SHRVVLAPLT RSRSYGNIPQ PNAKLYYTQR TTPGGLLISE
SCVVSETSLG YPDLPGLWNR DQVEAWKPIV DAVHSKGGIF FCQIWHGGRV FHQDQPNGEA
PVSSTDKPLM CKNMYGGQFK PPRRLRSDEL PAIVNDFRIA ARNAIEAGFD GVEVHGAHGY
LIDQFLKDKV NDRSDQYGGS LENRCRFALE VIEAVVNEIG SDRVGIRLSP FADYMESGDS
NPEALGLYLV QAMNKHGMES STVTWLNLE
