ORLIK_ARATH
ID ORLIK_ARATH Reviewed; 315 AA.
AC Q8VYD8; Q9LHS3;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protein ORANGE-LIKE, chloroplastic {ECO:0000305};
DE AltName: Full=DnaJ-like cysteine-rich domain-containing protein Or-like {ECO:0000305};
DE Flags: Precursor;
GN Name=ORLIKE; OrderedLocusNames=At5g06130 {ECO:0000312|Araport:AT5G06130};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, AND MUTAGENESIS OF ARG-97.
RX PubMed=26224804; DOI=10.1104/pp.15.00971;
RA Yuan H., Owsiany K., Sheeja T.E., Zhou X., Rodriguez C., Li Y., Welsch R.,
RA Chayut N., Yang Y., Thannhauser T.W., Parthasarathy M.V., Xu Q., Deng X.,
RA Fei Z., Schaffer A., Katzir N., Burger J., Tadmor Y., Li L.;
RT "A single amino acid substitution in an ORANGE protein promotes carotenoid
RT overaccumulation in Arabidopsis.";
RL Plant Physiol. 169:421-431(2015).
RN [5]
RP INTERACTION WITH PSY1, AND SUBCELLULAR LOCATION.
RX PubMed=25675505; DOI=10.1073/pnas.1420831112;
RA Zhou X., Welsch R., Yang Y., Alvarez D., Riediger M., Yuan H., Fish T.,
RA Liu J., Thannhauser T.W., Li L.;
RT "Arabidopsis OR proteins are the major posttranscriptional regulators of
RT phytoene synthase in controlling carotenoid biosynthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:3558-3563(2015).
CC -!- FUNCTION: May be associated with accumulation of carotenoids in
CC chromoplasts. {ECO:0000305|PubMed:26224804}.
CC -!- SUBUNIT: Interacts with PSY1. {ECO:0000269|PubMed:25675505}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:25675505}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences. {ECO:0000305};
CC Name=1;
CC IsoId=Q8VYD8-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the orange-like family.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA98202.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP002030; BAA98202.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90974.1; -; Genomic_DNA.
DR EMBL; AY072147; AAL59969.1; -; mRNA.
DR EMBL; AY133750; AAM91684.1; -; mRNA.
DR RefSeq; NP_196231.2; NM_120695.5. [Q8VYD8-1]
DR AlphaFoldDB; Q8VYD8; -.
DR SMR; Q8VYD8; -.
DR STRING; 3702.AT5G06130.2; -.
DR PaxDb; Q8VYD8; -.
DR PRIDE; Q8VYD8; -.
DR EnsemblPlants; AT5G06130.2; AT5G06130.2; AT5G06130. [Q8VYD8-1]
DR GeneID; 830500; -.
DR Gramene; AT5G06130.2; AT5G06130.2; AT5G06130. [Q8VYD8-1]
DR KEGG; ath:AT5G06130; -.
DR Araport; AT5G06130; -.
DR TAIR; locus:2152845; AT5G06130.
DR eggNOG; ENOG502QSP0; Eukaryota.
DR InParanoid; Q8VYD8; -.
DR PhylomeDB; Q8VYD8; -.
DR PRO; PR:Q8VYD8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8VYD8; baseline and differential.
DR GO; GO:0031969; C:chloroplast membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:1904143; P:positive regulation of carotenoid biosynthetic process; IGI:TAIR.
DR GO; GO:0050821; P:protein stabilization; IGI:TAIR.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Membrane; Metal-binding; Plastid;
KW Reference proteome; Repeat; Transit peptide; Transmembrane;
KW Transmembrane helix; Zinc; Zinc-finger.
FT TRANSIT 1..16
FT /note="Chloroplast"
FT /evidence="ECO:0000305"
FT CHAIN 17..315
FT /note="Protein ORANGE-LIKE, chloroplastic"
FT /id="PRO_0000438013"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 238..245
FT /note="CXXCXGXG motif"
FT /evidence="ECO:0000305"
FT REPEAT 249..256
FT /note="CXXCXXXG motif"
FT /evidence="ECO:0000305"
FT REPEAT 282..289
FT /note="CXXCXGXG motif"
FT /evidence="ECO:0000305"
FT REPEAT 293..300
FT /note="CXXCXXXG motif"
FT /evidence="ECO:0000305"
FT ZN_FING 225..307
FT /note="CR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00546"
FT MUTAGEN 97
FT /note="R->H: No effect on the accumulation of carotenoids."
FT /evidence="ECO:0000269|PubMed:26224804"
SQ SEQUENCE 315 AA; 34516 MW; CB245DE2FE5DB1AF CRC64;
MTCFSSATPH RHHLLLSSPS TSKSLLRFPS SYLKPSPSLL FHGSSRSLLS CSDGSNNRPP
PSGDTVPNNF CIIEGSETVQ DFVQMQLQEI QDNIRSRRNK IFLLMEEVRR LRVQQRIKSV
KAINEDSELE ATEMPEITSS IPFLPNVTPK TLKQLYSTSV ALISGIIFFG GLIAPNLELK
VGLGGTSYED FIRSLHLPLQ LSQVDPIVAS FSGGAVGVIS TLMLIEVNNV KQQEKKRCKY
CLGTGYLPCA RCSASGVCLS IDPITRPRAT NQLMQVATTK RCLNCSGAGK VMCPTCLCTG
MVTASEHDPR FDPFD
