ORMDL_DROME
ID ORMDL_DROME Reviewed; 154 AA.
AC Q9VP04; A0JQ27; Q8MX27;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=ORM1-like protein;
DE AltName: Full=dORMDL;
GN Name=ORMDL; ORFNames=CG14577;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Kapadia B.,
RA Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 9-137, AND TISSUE SPECIFICITY.
RX PubMed=12093374; DOI=10.1186/gb-2002-3-6-research0027;
RA Hjelmqvist L., Tuson M., Marfany G., Herrero E., Balcells S.,
RA Gonzalez-Duarte R.;
RT "ORMDL proteins are a conserved new family of endoplasmic reticulum
RT membrane proteins.";
RL Genome Biol. 3:RESEARCH0027.1-RESEARCH0027.13(2002).
CC -!- FUNCTION: Negative regulator of sphingolipid synthesis. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitously and homogeneously expressed in the
CC syncytial blastoderm and during the cellularization stage. In stages
CC 11-12, it is expressed in the germ-band layer In later stages (stage
CC 14), it is mainly detected in the ectodermal tissues. In imaginal disks
CC of third-instar larvae, it is uniformly and homogeneously expressed in
CC eye-antenna, leg, wing and haltere imaginal disks, which is consistent
CC with the former ectodermal expression detected in latestage embryos.
CC {ECO:0000269|PubMed:12093374}.
CC -!- SIMILARITY: Belongs to the ORM family. {ECO:0000305}.
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DR EMBL; AE014296; AAF51758.1; -; Genomic_DNA.
DR EMBL; BT023364; AAY55780.1; -; mRNA.
DR EMBL; BT029397; ABK56983.1; -; mRNA.
DR EMBL; AF395703; AAM43501.1; -; mRNA.
DR RefSeq; NP_730669.1; NM_168929.2.
DR AlphaFoldDB; Q9VP04; -.
DR SMR; Q9VP04; -.
DR BioGRID; 65648; 1.
DR IntAct; Q9VP04; 18.
DR STRING; 7227.FBpp0078077; -.
DR GlyGen; Q9VP04; 2 sites.
DR PaxDb; Q9VP04; -.
DR PRIDE; Q9VP04; -.
DR DNASU; 40404; -.
DR EnsemblMetazoa; FBtr0078423; FBpp0078077; FBgn0037110.
DR GeneID; 40404; -.
DR KEGG; dme:Dmel_CG14577; -.
DR UCSC; CG14577-RA; d. melanogaster.
DR CTD; 40404; -.
DR FlyBase; FBgn0037110; ORMDL.
DR VEuPathDB; VectorBase:FBgn0037110; -.
DR eggNOG; KOG3319; Eukaryota.
DR GeneTree; ENSGT00950000183178; -.
DR HOGENOM; CLU_072117_3_0_1; -.
DR InParanoid; Q9VP04; -.
DR OMA; STHYTHF; -.
DR OrthoDB; 1405600at2759; -.
DR PhylomeDB; Q9VP04; -.
DR Reactome; R-DME-1660661; Sphingolipid de novo biosynthesis.
DR Reactome; R-DME-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 40404; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 40404; -.
DR PRO; PR:Q9VP04; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0037110; Expressed in adult Malpighian tubule (Drosophila) and 29 other tissues.
DR ExpressionAtlas; Q9VP04; baseline and differential.
DR Genevisible; Q9VP04; DM.
DR GO; GO:0005829; C:cytosol; HDA:FlyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0035339; C:SPOTS complex; IBA:GO_Central.
DR GO; GO:0090156; P:cellular sphingolipid homeostasis; IBA:GO_Central.
DR GO; GO:0006672; P:ceramide metabolic process; IBA:GO_Central.
DR GO; GO:1900060; P:negative regulation of ceramide biosynthetic process; IBA:GO_Central.
DR InterPro; IPR007203; ORMDL.
DR InterPro; IPR029883; ORML3.
DR PANTHER; PTHR12665; PTHR12665; 1.
DR PANTHER; PTHR12665:SF11; PTHR12665:SF11; 1.
DR Pfam; PF04061; ORMDL; 1.
DR PIRSF; PIRSF018147; ORMDL; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..154
FT /note="ORM1-like protein"
FT /id="PRO_0000215644"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 14
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 120
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 154 AA; 17570 MW; 936139094061D668 CRC64;
MTSIAGGHGE ANPNSSWLSA RGFWLAYLLG LLSVHLLFLS VPFVSIPWAW TATNLLHNAA
HLYFLHVIKG APWLSTENDP SRRWTHWEQI DDGVQMTTTR KFLTAVPIVL FLLTCLYTRN
NTEHFIPNFI SLVVVTLPKL PQFHGVRLFN INKY
