ORN_YEAST
ID ORN_YEAST Reviewed; 269 AA.
AC P54964; D6VY61; E9P909;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Oligoribonuclease, mitochondrial;
DE EC=3.1.15.-;
DE Flags: Precursor;
GN Name=REX2; Synonyms=YNT20; OrderedLocusNames=YLR059C; ORFNames=L2159;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RA Hanekamp T., Thorsness P.E.;
RL Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP IDENTIFICATION, AND SUBCELLULAR LOCATION.
RX PubMed=9933355; DOI=10.1007/s002940050418;
RA Hanekamp T., Thorsness P.E.;
RT "YNT20, a bypass suppressor of yme1 yme2, encodes a putative 3'-5'
RT exonuclease localized in mitochondria of Saccharomyces cerevisiae.";
RL Curr. Genet. 34:438-448(1999).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: 3'-to-5' exoribonuclease specific for small
CC oligoribonucleotides. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:9933355}.
CC -!- MISCELLANEOUS: Present with 1730 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the oligoribonuclease family. {ECO:0000305}.
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DR EMBL; L47977; AAA98633.1; -; Genomic_DNA.
DR EMBL; X94607; CAA64306.1; -; Genomic_DNA.
DR EMBL; Z73231; CAA97590.1; -; Genomic_DNA.
DR EMBL; AY693005; AAT93024.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09377.1; -; Genomic_DNA.
DR PIR; S61633; S61633.
DR RefSeq; NP_013160.1; NM_001181946.1.
DR AlphaFoldDB; P54964; -.
DR SMR; P54964; -.
DR BioGRID; 31334; 101.
DR DIP; DIP-5084N; -.
DR IntAct; P54964; 5.
DR STRING; 4932.YLR059C; -.
DR iPTMnet; P54964; -.
DR MaxQB; P54964; -.
DR PaxDb; P54964; -.
DR PRIDE; P54964; -.
DR EnsemblFungi; YLR059C_mRNA; YLR059C; YLR059C.
DR GeneID; 850748; -.
DR KEGG; sce:YLR059C; -.
DR SGD; S000004049; REX2.
DR VEuPathDB; FungiDB:YLR059C; -.
DR eggNOG; KOG3242; Eukaryota.
DR GeneTree; ENSGT00390000009255; -.
DR HOGENOM; CLU_064761_0_2_1; -.
DR InParanoid; P54964; -.
DR OMA; GKKHRAM; -.
DR BioCyc; YEAST:G3O-32213-MON; -.
DR PRO; PR:P54964; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; P54964; protein.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0008408; F:3'-5' exonuclease activity; ISS:SGD.
DR GO; GO:0000175; F:3'-5'-exoribonuclease activity; IMP:SGD.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IGI:SGD.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR GO; GO:0034475; P:U4 snRNA 3'-end processing; IMP:SGD.
DR GO; GO:0034476; P:U5 snRNA 3'-end processing; IGI:SGD.
DR CDD; cd06135; Orn; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR022894; Oligoribonuclease.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR11046; PTHR11046; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 1: Evidence at protein level;
KW Exonuclease; Hydrolase; Mitochondrion; Nuclease; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..269
FT /note="Oligoribonuclease, mitochondrial"
FT /id="PRO_0000020274"
FT DOMAIN 55..227
FT /note="Exonuclease"
FT REGION 240..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 184
FT /evidence="ECO:0000255"
FT CONFLICT 100
FT /note="S -> T (in Ref. 1; AAA98633)"
FT /evidence="ECO:0000305"
FT CONFLICT 124..127
FT /note="GLTA -> ESHP (in Ref. 1; AAA98633)"
FT /evidence="ECO:0000305"
FT CONFLICT 181
FT /note="H -> R (in Ref. 4; AAT93024)"
FT /evidence="ECO:0000305"
FT CONFLICT 223..269
FT /note="SIAQLQWYMDNYLKPPQETESVESIGSEQPESPSSSTSSLKRQRTDF -> H
FT SSIAMVHGQLLEATTGNRVGRVNRI (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 269 AA; 30870 MW; C942C823691E9815 CRC64;
MKWLLFPARI VARTRPNLFS LYRRSVSQYL RPRTIQNLQS MAQTPELKTK LFKPLVWIDC
EMTGLDHVND RIIEICCIIT DGHLAPVKAA DGQGDSHYES VIHYGPEVMN KMNEWCIEHH
GNSGLTAKVL ASEKTLAQVE DELLEYIQRY IPDKNVGVLA GNSVHMDRLF MVREFPKVID
HLFYRIVDVS SIMEVARRHN PALQARNPKK EAAHTAYSDI KESIAQLQWY MDNYLKPPQE
TESVESIGSE QPESPSSSTS SLKRQRTDF
