ID   ORORA_CUCME             Reviewed;         325 AA.
AC   A0A0D3MU50;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   27-MAY-2015, sequence version 1.
DT   03-AUG-2022, entry version 17.
DE   RecName: Full=Protein ORANGE-ORANGE, chloroplastic {ECO:0000305};
DE            Short=CmOr-orange {ECO:0000303|PubMed:25754094};
DE   AltName: Full=DnaJ-like cysteine-rich domain-containing protein Or {ECO:0000303|PubMed:25754094};
DE   Flags: Precursor;
GN   Name=OR {ECO:0000303|PubMed:25754094};
OS   Cucumis melo (Muskmelon).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX   NCBI_TaxID=3656;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION,
RP   DEVELOPMENTAL STAGE, AND POLYMORPHISM.
RX   PubMed=25754094; DOI=10.1111/tpj.12814;
RA   Tzuri G., Zhou X., Chayut N., Yuan H., Portnoy V., Meir A., Sa'ar U.,
RA   Baumkoler F., Mazourek M., Lewinsohn E., Fei Z., Schaffer A.A., Li L.,
RA   Burger J., Katzir N., Tadmor Y.;
RT   "A 'golden' SNP in CmOr governs the fruit flesh color of melon (Cucumis
RT   melo).";
RL   Plant J. 82:267-279(2015).
CC   -!- FUNCTION: Triggers accumulation of carotenoids, mainly beta-carotene,
CC       in fruit flesh. {ECO:0000269|PubMed:25754094}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC       {ECO:0000305|PubMed:25754094}.
CC   -!- DEVELOPMENTAL STAGE: Expression increases in fruit flesh during fruit
CC       development. {ECO:0000269|PubMed:25754094}.
CC   -!- POLYMORPHISM: The OR gene possesses two alleles in melon, one
CC       associated with orange flesh (this entry) and the second being
CC       associated with either white or green flesh (AC A0A0D3MU35). A single
CC       SNP between the two alleles causes a change of an evolutionarily highly
CC       conserved Arg in position 108 (AC A0A0D3MU35) to His (His-108). The Arg
CC       and His alleles are responsible for the non-orange and orange melon
CC       fruit phenotypes, respectively. This findings could serve as a novel
CC       genetic tool to enrich carotenoid content in transgenic crops.
CC       {ECO:0000305|PubMed:25754094}.
CC   -!- MISCELLANEOUS: Its sequence is related to the DnaJ family but lacks the
CC       J domain. The CR-type-like region is similar to CR-type zinc-fingers.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the orange-like family.
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DR   EMBL; KM505046; AIZ95444.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0D3MU50; -.
DR   SMR; A0A0D3MU50; -.
DR   Proteomes; UP000089565; Unplaced.
DR   GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IEA:EnsemblPlants.
DR   GO; GO:0009658; P:chloroplast organization; IEA:EnsemblPlants.
DR   GO; GO:1904143; P:positive regulation of carotenoid biosynthetic process; IMP:UniProtKB.
DR   GO; GO:0050821; P:protein stabilization; IEA:EnsemblPlants.
PE   2: Evidence at transcript level;
KW   Chloroplast; Membrane; Plastid; Reference proteome; Repeat;
KW   Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..54
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           55..325
FT                   /note="Protein ORANGE-ORANGE, chloroplastic"
FT                   /id="PRO_0000438016"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        217..237
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          248..255
FT                   /note="CXXCXGXG motif"
FT                   /evidence="ECO:0000305"
FT   REPEAT          259..266
FT                   /note="CXXCXXXG motif"
FT                   /evidence="ECO:0000305"
FT   REPEAT          292..299
FT                   /note="CXXCXGXG motif"
FT                   /evidence="ECO:0000305"
FT   REPEAT          303..310
FT                   /note="CXXCXXXG motif"
FT                   /evidence="ECO:0000305"
FT   REGION          53..77
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          226..317
FT                   /note="CR-type-like"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   325 AA;  36251 MW;  4A56FF222AEE0033 CRC64;
     MDRVLVASYP INHLIRPHSF RIDYCWSTCF TSRLNSGKER QKLSSRWRWR SMASDSTDSS
     SSSSFAPSVE SDPSDKTSAS FCIIEGPETV QDFAKMELQE IQENIRSHRN KIFLHMEEVR
     RLRIQQRIKN AELGISKEER ENELPNFPSF IPFLPPLSSE NLKLYYVTCY SLIAGIILFG
     GLLAPTLELK LGLGGTSYED FIRSVHLPMQ LSQVDPIVAS FSGGAVGVIS ALMVVEVNNV
     KQQEHKRCKY CLGTGYLACA RCSNTGALVL IEPVSTLNGE HQPLSLPKTE RCQNCSGSGK
     VMCPTCLCTG MAMASEHDPR IDPFD