ORP2A_ARATH
ID ORP2A_ARATH Reviewed; 721 AA.
AC Q940Y1; Q0WMK9; Q56W69; Q9SUW5;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Oxysterol-binding protein-related protein 2A;
DE AltName: Full=OSBP-related protein 2A;
GN Name=ORP2A; OrderedLocusNames=At4g22540; ORFNames=F7K2.120;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 318-721.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16897474; DOI=10.1007/s11103-006-0030-y;
RA Skirpan A.L., Dowd P.E., Sijacic P., Jaworski C.J., Gilroy S., Kao T.H.;
RT "Identification and characterization of PiORP1, a Petunia oxysterol-
RT binding-protein related protein involved in receptor-kinase mediated
RT signaling in pollen, and analysis of the ORP gene family in Arabidopsis.";
RL Plant Mol. Biol. 61:553-565(2006).
CC -!- FUNCTION: May be involved in the transport of sterols. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q940Y1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q940Y1-2; Sequence=VSP_040249;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems and flowers.
CC {ECO:0000269|PubMed:16897474}.
CC -!- SIMILARITY: Belongs to the OSBP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA22159.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79209.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL033545; CAA22159.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161557; CAB79209.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84619.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84620.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84621.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84622.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66311.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66312.1; -; Genomic_DNA.
DR EMBL; AK229811; BAF01642.1; -; mRNA.
DR EMBL; AY052361; AAK96552.1; -; mRNA.
DR EMBL; BT001093; AAN46892.1; -; mRNA.
DR EMBL; AK222178; BAD95307.1; -; mRNA.
DR PIR; T05448; T05448.
DR RefSeq; NP_001031698.1; NM_001036621.3. [Q940Y1-2]
DR RefSeq; NP_001190800.1; NM_001203871.1. [Q940Y1-2]
DR RefSeq; NP_001328217.1; NM_001341552.1. [Q940Y1-1]
DR RefSeq; NP_001328218.1; NM_001341553.1. [Q940Y1-2]
DR RefSeq; NP_567662.1; NM_118380.3. [Q940Y1-1]
DR RefSeq; NP_974592.1; NM_202863.3. [Q940Y1-2]
DR AlphaFoldDB; Q940Y1; -.
DR SMR; Q940Y1; -.
DR BioGRID; 13638; 2.
DR IntAct; Q940Y1; 1.
DR STRING; 3702.AT4G22540.1; -.
DR PaxDb; Q940Y1; -.
DR PRIDE; Q940Y1; -.
DR ProteomicsDB; 248645; -. [Q940Y1-1]
DR EnsemblPlants; AT4G22540.1; AT4G22540.1; AT4G22540. [Q940Y1-1]
DR EnsemblPlants; AT4G22540.2; AT4G22540.2; AT4G22540. [Q940Y1-2]
DR EnsemblPlants; AT4G22540.3; AT4G22540.3; AT4G22540. [Q940Y1-2]
DR EnsemblPlants; AT4G22540.4; AT4G22540.4; AT4G22540. [Q940Y1-2]
DR EnsemblPlants; AT4G22540.5; AT4G22540.5; AT4G22540. [Q940Y1-1]
DR EnsemblPlants; AT4G22540.6; AT4G22540.6; AT4G22540. [Q940Y1-2]
DR GeneID; 828349; -.
DR Gramene; AT4G22540.1; AT4G22540.1; AT4G22540. [Q940Y1-1]
DR Gramene; AT4G22540.2; AT4G22540.2; AT4G22540. [Q940Y1-2]
DR Gramene; AT4G22540.3; AT4G22540.3; AT4G22540. [Q940Y1-2]
DR Gramene; AT4G22540.4; AT4G22540.4; AT4G22540. [Q940Y1-2]
DR Gramene; AT4G22540.5; AT4G22540.5; AT4G22540. [Q940Y1-1]
DR Gramene; AT4G22540.6; AT4G22540.6; AT4G22540. [Q940Y1-2]
DR KEGG; ath:AT4G22540; -.
DR Araport; AT4G22540; -.
DR TAIR; locus:2127565; AT4G22540.
DR eggNOG; KOG1737; Eukaryota.
DR HOGENOM; CLU_007105_0_1_1; -.
DR InParanoid; Q940Y1; -.
DR PhylomeDB; Q940Y1; -.
DR PRO; PR:Q940Y1; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q940Y1; baseline and differential.
DR Genevisible; Q940Y1; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0032934; F:sterol binding; IBA:GO_Central.
DR GO; GO:0015248; F:sterol transporter activity; IBA:GO_Central.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR037239; OSBP_sf.
DR InterPro; IPR000648; Oxysterol-bd.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR10972; PTHR10972; 1.
DR Pfam; PF01237; Oxysterol_BP; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF144000; SSF144000; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Lipid transport; Lipid-binding;
KW Reference proteome; Transport.
FT CHAIN 1..721
FT /note="Oxysterol-binding protein-related protein 2A"
FT /id="PRO_0000402160"
FT DOMAIN 50..177
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 20..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 274..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 640..669
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..211
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074"
FT /id="VSP_040249"
SQ SEQUENCE 721 AA; 82042 MW; 8B1DC7DE6F67D14C CRC64;
MRVKELHPLC CITLESPHGI NSQSPEEPVT TALTRSRSLP AKSLTGGSES ETVAGILYKW
TNFGKGWRSR WFLLRNGILS YSKIRRPENL NLLSPDDDVR LIGDISGERL SRMDSCSGRR
KQQKTVGIVH LKVSSFRESK SDDRKFYIFT ATKTLHLRTD SITDRAAWLQ ALASTKCIFP
LRSLNGDFSF IPPKDLSIST ERLKKRLHEE GMNENLVKEC EQIMLSEFSE MHGQVKLLHE
ERTNLLDALR QLEAANLEVG ASGKHEFSSL GRGKYSECST TASSDGKQEF EDVSEEDEPS
FHDTKEFFNE PNIGSESNLA ISGHTDIKRR TKLPDPAEKE KGVSLWSMIK DNVGKDLTRV
CLPVYFNEPI SSLQKCFEDL EYSYLLDRAY EHGKSGNGLL RALNVAAFAV SGYASTEGRH
CKPFNPLLGE TYEADFPEKG IRFFSEKVSH HPTVIACHCE GKGWKFWGDT NLRSKFWGRS
IQVEPVGVLT LEFDDGEVFQ WSKVTSTIYN IILGKLYCDH HGVMQIRGNR QYSCTLKFKE
QSILERNPHQ VNGFVEDVAG QKAATIFGKW DDSLYYVAGD GINKSKVSDP ASNASLLWKR
TKPPPNVTRY NLTSFAITLN ELTPGLQEIL PPTDSRLRPD QRHLENGEYE KANLEKQRLE
RRQRMSRQLQ ESGWRPRWFE RQGESDTFKY TGGYWEARGH RNWDDCPDIF GEFTEELADA
A
