ORP2B_ARATH
ID ORP2B_ARATH Reviewed; 693 AA.
AC Q9SU36; Q8L711;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Oxysterol-binding protein-related protein 2B;
DE AltName: Full=OSBP-related protein 2B;
GN Name=ORP2B; OrderedLocusNames=At4g12460; ORFNames=T1P17.50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16897474; DOI=10.1007/s11103-006-0030-y;
RA Skirpan A.L., Dowd P.E., Sijacic P., Jaworski C.J., Gilroy S., Kao T.H.;
RT "Identification and characterization of PiORP1, a Petunia oxysterol-
RT binding-protein related protein involved in receptor-kinase mediated
RT signaling in pollen, and analysis of the ORP gene family in Arabidopsis.";
RL Plant Mol. Biol. 61:553-565(2006).
CC -!- FUNCTION: May be involved in the transport of sterols. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SU36-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SU36-2; Sequence=VSP_040250, VSP_040251;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems and flowers.
CC {ECO:0000269|PubMed:16897474}.
CC -!- SIMILARITY: Belongs to the OSBP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB41716.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78289.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL049730; CAB41716.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161534; CAB78289.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83135.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83136.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66036.1; -; Genomic_DNA.
DR EMBL; AY140023; AAM98164.1; -; mRNA.
DR EMBL; BT008852; AAP68291.1; -; mRNA.
DR PIR; T07638; T07638.
DR RefSeq; NP_001078376.1; NM_001084907.1. [Q9SU36-2]
DR RefSeq; NP_001327962.1; NM_001340780.1. [Q9SU36-1]
DR RefSeq; NP_001327968.1; NM_001340779.1.
DR RefSeq; NP_192983.2; NM_117316.3. [Q9SU36-1]
DR AlphaFoldDB; Q9SU36; -.
DR SMR; Q9SU36; -.
DR BioGRID; 12156; 1.
DR IntAct; Q9SU36; 1.
DR STRING; 3702.AT4G12460.1; -.
DR iPTMnet; Q9SU36; -.
DR PaxDb; Q9SU36; -.
DR PRIDE; Q9SU36; -.
DR ProteomicsDB; 248906; -. [Q9SU36-1]
DR EnsemblPlants; AT4G12460.1; AT4G12460.1; AT4G12460. [Q9SU36-1]
DR EnsemblPlants; AT4G12460.2; AT4G12460.2; AT4G12460. [Q9SU36-2]
DR EnsemblPlants; AT4G12460.8; AT4G12460.8; AT4G12460. [Q9SU36-1]
DR GeneID; 826858; -.
DR Gramene; AT4G12460.1; AT4G12460.1; AT4G12460. [Q9SU36-1]
DR Gramene; AT4G12460.2; AT4G12460.2; AT4G12460. [Q9SU36-2]
DR Gramene; AT4G12460.8; AT4G12460.8; AT4G12460. [Q9SU36-1]
DR KEGG; ath:AT4G12460; -.
DR Araport; AT4G12460; -.
DR TAIR; locus:2135580; AT4G12460.
DR eggNOG; KOG1737; Eukaryota.
DR HOGENOM; CLU_007105_0_1_1; -.
DR InParanoid; Q9SU36; -.
DR OrthoDB; 542090at2759; -.
DR PhylomeDB; Q9SU36; -.
DR PRO; PR:Q9SU36; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SU36; baseline and differential.
DR Genevisible; Q9SU36; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0032934; F:sterol binding; IBA:GO_Central.
DR GO; GO:0015248; F:sterol transporter activity; IBA:GO_Central.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR037239; OSBP_sf.
DR InterPro; IPR000648; Oxysterol-bd.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR10972; PTHR10972; 1.
DR Pfam; PF01237; Oxysterol_BP; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF144000; SSF144000; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Lipid transport; Lipid-binding;
KW Reference proteome; Transport.
FT CHAIN 1..693
FT /note="Oxysterol-binding protein-related protein 2B"
FT /id="PRO_0000402161"
FT DOMAIN 25..154
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 256..298
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 600..639
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 207..239
FT /evidence="ECO:0000255"
FT COILED 612..643
FT /evidence="ECO:0000255"
FT COMPBIAS 603..639
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..326
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_040250"
FT VAR_SEQ 600..601
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_040251"
SQ SEQUENCE 693 AA; 79566 MW; 0FF3F23AF76C9229 CRC64;
MPLTRSKSLP ATENGGSDRE TLQSGRSVAG ILYKWTNYGK GWRSRWFLLR DGILSYSKIR
RPENVNLLSP SDDLRLIGDI STDRLLRMKS CSGRSRRKHH KNIGIVHLKV SSYRESKSDH
RKFYIFTATK TLHLRTDSRS DRAAWLQALA STRGIVPLQS INGDFSFVSP KDLSISTERL
KKRLFEEGMN ESLVKECEQI VDSEFCEVQE QIKLLHEERK KLLDALRQLE MANLEAEASG
IHDDVYQLRN HKYSSLGRGK YSECSTSASS DDKQEFEDIS EEDEASFHDT KESFGEPDVG
SVLTHFKRRT KLPDPAEKER GVSLWSMIKD NVGKDLTRVC LPVYFNEPIS SLQKCFEDLE
YSYLLDQAYE YGKSGKSLLR ALNVAAFAVS GYASTEGRHC KPFNPLLGET YEADFPEKGI
RFFSEKVSHH PTVIACHCEG KGWKFWGDTN LRSKFWGRSI QLEPVGILTL EFDDGEIFQW
SKVTTTIYNI LLGKLYCDHH GIMKIRGNRQ YSCMLKFKEQ SILDRNPHQV NGFVEDVTGK
KAATVFGKWN DSLYYVAGDG INKASASLLW KATKAPPNVT RYNFTSFAMT LNELIPGLEE
KLPPTDSRLR PDQRHLENGE YEKANEEKQR LERRQRMSRQ IQESGWRPRW FEPQGESESY
KYTGGYWEAR DVKSWDDCPN IFGEFTEEVA DCA