ASB1_MOUSE
ID ASB1_MOUSE Reviewed; 336 AA.
AC Q9WV74;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Ankyrin repeat and SOCS box protein 1;
DE Short=ASB-1;
GN Name=Asb1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=11111040; DOI=10.1016/s0378-1119(00)00402-9;
RA Kile B.T., Viney E.M., Willson T.A., Brodnicki T.C., Cancilla M.R.,
RA Herlihy A.S., Croker B.A., Baca M., Nicola N.A., Hilton D.J.,
RA Alexander W.S.;
RT "Cloning and characterization of the genes encoding the ankyrin repeat and
RT SOCS box-containing proteins Asb-1, Asb-2, Asb-3 and Asb-4.";
RL Gene 258:31-41(2000).
RN [2]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=11509662; DOI=10.1128/mcb.21.18.6189-6197.2001;
RA Kile B.T., Metcalf D., Mifsud S., DiRago L., Nicola N.A., Hilton D.J.,
RA Alexander W.S.;
RT "Functional analysis of Asb-1 using genetic modification in mice.";
RL Mol. Cell. Biol. 21:6189-6197(2001).
CC -!- FUNCTION: Probable substrate-recognition component of a SCF-like ECS
CC (Elongin-Cullin-SOCS-box protein) E3 ligase complex which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins (By similarity). Mediates Notch-induced ubiquitination and
CC degradation of TCF3/E2A and JAK2 (By similarity). May play a role in
CC testis development (PubMed:11509662). {ECO:0000250|UniProtKB:Q9Y576,
CC ECO:0000269|PubMed:11509662}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Interacts with CUL5 and RNF7. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Highest expression in testis, spleen, bone marrow
CC and salivary gland. {ECO:0000269|PubMed:11111040,
CC ECO:0000269|PubMed:11509662}.
CC -!- DOMAIN: The SOCS box domain mediates the interaction with the Elongin
CC BC complex, an adapter module in different E3 ubiquitin-protein ligase
CC complexes. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Decreased spermatogenesis with less complete
CC filling of seminiferous tubules but fertility does not appear to be
CC affected. {ECO:0000269|PubMed:11509662}.
CC -!- SIMILARITY: Belongs to the ankyrin SOCS box (ASB) family.
CC {ECO:0000305}.
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DR EMBL; AF155352; AAD38808.1; -; mRNA.
DR CCDS; CCDS35665.1; -.
DR RefSeq; NP_001034215.1; NM_001039126.2.
DR AlphaFoldDB; Q9WV74; -.
DR SMR; Q9WV74; -.
DR BioGRID; 211142; 3.
DR IntAct; Q9WV74; 2.
DR STRING; 10090.ENSMUSP00000084054; -.
DR PhosphoSitePlus; Q9WV74; -.
DR MaxQB; Q9WV74; -.
DR PaxDb; Q9WV74; -.
DR PRIDE; Q9WV74; -.
DR ProteomicsDB; 283183; -.
DR Antibodypedia; 1137; 31 antibodies from 12 providers.
DR DNASU; 65247; -.
DR Ensembl; ENSMUST00000086843; ENSMUSP00000084054; ENSMUSG00000026311.
DR GeneID; 65247; -.
DR KEGG; mmu:65247; -.
DR UCSC; uc007caz.1; mouse.
DR CTD; 51665; -.
DR MGI; MGI:1929735; Asb1.
DR VEuPathDB; HostDB:ENSMUSG00000026311; -.
DR eggNOG; KOG0504; Eukaryota.
DR GeneTree; ENSGT00940000153969; -.
DR HOGENOM; CLU_053981_0_0_1; -.
DR InParanoid; Q9WV74; -.
DR OMA; TGPVCQE; -.
DR OrthoDB; 1048859at2759; -.
DR PhylomeDB; Q9WV74; -.
DR TreeFam; TF331945; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 65247; 0 hits in 74 CRISPR screens.
DR PRO; PR:Q9WV74; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q9WV74; protein.
DR Bgee; ENSMUSG00000026311; Expressed in blood and 204 other tissues.
DR ExpressionAtlas; Q9WV74; baseline and differential.
DR Genevisible; Q9WV74; MM.
DR GO; GO:0000151; C:ubiquitin ligase complex; ISO:MGI.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; ISO:MGI.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0030539; P:male genitalia development; IMP:MGI.
DR GO; GO:0016567; P:protein ubiquitination; ISO:MGI.
DR CDD; cd03720; SOCS_ASB1; 1.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR037331; ASB1_SOCS.
DR InterPro; IPR001496; SOCS_box.
DR InterPro; IPR036036; SOCS_box-like_dom_sf.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF07525; SOCS_box; 1.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 5.
DR SMART; SM00969; SOCS_box; 1.
DR SUPFAM; SSF158235; SSF158235; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 3.
DR PROSITE; PS50225; SOCS; 1.
PE 2: Evidence at transcript level;
KW ANK repeat; Developmental protein; Reference proteome; Repeat;
KW Ubl conjugation pathway.
FT CHAIN 1..336
FT /note="Ankyrin repeat and SOCS box protein 1"
FT /id="PRO_0000066924"
FT REPEAT 37..69
FT /note="ANK 1"
FT REPEAT 78..107
FT /note="ANK 2"
FT REPEAT 111..140
FT /note="ANK 3"
FT REPEAT 144..173
FT /note="ANK 4"
FT REPEAT 192..221
FT /note="ANK 5"
FT REPEAT 236..266
FT /note="ANK 6"
FT DOMAIN 287..336
FT /note="SOCS box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00194"
SQ SEQUENCE 336 AA; 37232 MW; 966119E1B21A5E4F CRC64;
MAEGGTGPDG RAGPGPAGPN LKEWLREQFC DHPLEHCDDT RLHDAAYVGD LQTLRNLLQE
ESYRSRINEK SVWCCGWLPC TPLRIAATAG HGNCVDFLIR KGAEVDLVDV KGQTALYVAV
VNGHLESTEI LLEAGADPNG SRHHRSTPVY HASRVGRDDI LKALIRYGAD VDVNHHLTPD
TRPPFSRRLT SLVVCPLYIS AAYHNLQCFR LLLQAGANPD FNCNGPVNTQ EFYRGSPGCV
MDAVLRHGCE AAFVSLLVEF GANLNLVKWE SLGPEARGRR KMDPEALQVF KEARSIPRTL
LSLCRVAVRR ALGKYRLHLV PSLPLPDPIK KFLLYE
