ID   ORYA_ORYSJ              Reviewed;         458 AA.
AC   P25776; Q25AN0; Q7XMQ3;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 2.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Oryzain alpha chain;
DE            EC=3.4.22.-;
DE   Flags: Precursor;
GN   OrderedLocusNames=Os04g0650000, LOC_Os04g55650;
GN   ORFNames=H0212B02.7, OSJNBb0059K02.8;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 129-135.
RC   STRAIN=cv. Nipponbare; TISSUE=Seed;
RX   PubMed=1885617; DOI=10.1016/s0021-9258(18)55387-4;
RA   Watanabe H., Abe K., Emori Y., Hosoyama H., Arai S.;
RT   "Molecular cloning and gibberellin-induced expression of multiple cysteine
RT   proteinases of rice seeds (oryzains).";
RL   J. Biol. Chem. 266:16897-16902(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- TISSUE SPECIFICITY: Expressed only in seeds.
CC   -!- INDUCTION: By gibberellic acid (GA).
CC   -!- SIMILARITY: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU10088, ECO:0000255|PROSITE-ProRule:PRU10089,
CC       ECO:0000255|PROSITE-ProRule:PRU10090}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAE04498.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAJ86180.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; D90406; BAA14402.1; -; mRNA.
DR   EMBL; AL442007; CAJ86180.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL606692; CAE04498.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014960; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK071913; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; JU0388; KHRZOA.
DR   AlphaFoldDB; P25776; -.
DR   SMR; P25776; -.
DR   IntAct; P25776; 1.
DR   STRING; 4530.OS04T0650000-03; -.
DR   MEROPS; C01.029; -.
DR   PaxDb; P25776; -.
DR   PRIDE; P25776; -.
DR   eggNOG; KOG1543; Eukaryota.
DR   eggNOG; KOG4296; Eukaryota.
DR   InParanoid; P25776; -.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005764; C:lysosome; IBA:GO_Central.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0051603; P:proteolysis involved in protein catabolic process; IBA:GO_Central.
DR   CDD; cd02248; Peptidase_C1A; 1.
DR   Gene3D; 2.10.25.160; -; 1.
DR   InterPro; IPR000118; Granulin.
DR   InterPro; IPR037277; Granulin_sf.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR025661; Pept_asp_AS.
DR   InterPro; IPR000169; Pept_cys_AS.
DR   InterPro; IPR025660; Pept_his_AS.
DR   InterPro; IPR000668; Peptidase_C1A_C.
DR   InterPro; IPR039417; Peptidase_C1A_papain-like.
DR   InterPro; IPR013201; Prot_inhib_I29.
DR   Pfam; PF00396; Granulin; 1.
DR   Pfam; PF08246; Inhibitor_I29; 1.
DR   Pfam; PF00112; Peptidase_C1; 1.
DR   PRINTS; PR00705; PAPAIN.
DR   SMART; SM00277; GRAN; 1.
DR   SMART; SM00848; Inhibitor_I29; 1.
DR   SMART; SM00645; Pept_C1; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS00640; THIOL_PROTEASE_ASN; 1.
DR   PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
DR   PROSITE; PS00639; THIOL_PROTEASE_HIS; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Hydrolase;
KW   Protease; Reference proteome; Signal; Thiol protease; Zymogen.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..128
FT                   /note="Activation peptide"
FT                   /evidence="ECO:0000269|PubMed:1885617"
FT                   /id="PRO_0000026432"
FT   CHAIN           129..348
FT                   /note="Oryzain alpha chain"
FT                   /id="PRO_0000026433"
FT   PROPEP          349..458
FT                   /note="Removed in mature form"
FT                   /id="PRO_0000046020"
FT   ACT_SITE        153
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        289
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        309
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        445
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        150..192
FT                   /evidence="ECO:0000250"
FT   DISULFID        184..225
FT                   /evidence="ECO:0000250"
FT   DISULFID        283..334
FT                   /evidence="ECO:0000250"
FT   DISULFID        367..379
FT                   /evidence="ECO:0000250"
FT   DISULFID        373..394
FT                   /evidence="ECO:0000250"
FT   CONFLICT        164
FT                   /note="G -> D (in Ref. 1; BAA14402)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        256
FT                   /note="A -> R (in Ref. 1; BAA14402)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   458 AA;  50131 MW;  D713400DF5A28EB3 CRC64;
     MRISMALAAA ALLLLLSLAA ADMSIVSYGE RSEEEARRLY AEWKAEHGKS YNAVGEEERR
     YAAFRDNLRY IDEHNAAADA GVHSFRLGLN RFADLTNEEY RDTYLGLRNK PRRERKVSDR
     YLAADNEALP ESVDWRTKGA VAEIKDQGGC GSCWAFSAIA AVEGINQIVT GDLISLSEQE
     LVDCDTSYNE GCNGGLMDYA FDFIINNGGI DTEDDYPYKG KDERCDVNRK NAKVVTIDSY
     EDVTPNSETS LQKAVANQPV SVAIEAGGRA FQLYSSGIFT GKCGTALDHG VAAVGYGTEN
     GKDYWIVRNS WGKSWGESGY VRMERNIKAS SGKCGIAVEP SYPLKKGENP PNPGPTPPSP
     TPPPTVCDNY YTCPDSTTCC CIYEYGKYCY AWGCCPLEGA TCCDDHYSCC PHEYPICNVQ
     QGTCLMAKDS PLAVKALKRT LAKPNLSFLF GNGKKSSA