ID   ORYJ_ASPOR              Reviewed;         179 AA.
AC   Q2TXF5;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   12-AUG-2020, sequence version 2.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Oryzines biosynthesis cluster protein J {ECO:0000303|PubMed:30104550};
GN   Name=oryJ {ECO:0000303|PubMed:30104550}; ORFNames=AO090010000166;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
RN   [2]
RP   FUNCTION, AND PATHWAY.
RX   PubMed=30104550; DOI=10.3390/jof4030096;
RA   Wasil Z., Kuhnert E., Simpson T.J., Cox R.J.;
RT   "Oryzines A & B, maleidride congeners from Aspergillus oryzae and their
RT   putative biosynthesis.";
RL   J. Fungi 4:0-0(2018).
CC   -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC       oryzines, natural products with an unusual maleidride backbone
CC       (PubMed:30104550). The two subunits of the fungal fatty acid synthase
CC       oryfasA and oryfasB probably form octenoic acid (Probable). This fatty
CC       acid is most likely activated by the acyl-CoA ligase oryP to give
CC       octenyl-CoA before the citrate synthase-like protein oryE catalyzes
CC       condensation with oxaloacetate to form tricarboxylic acid (Probable).
CC       The next steps of the pathways are conjectural, but a favorite possible
CC       route has been proposed, beginning with decarboxylation and concomitant
CC       dehydration by the decarboxylase oryM, followed by tautomerization,
CC       which may lead to the production of a diene intermediate (Probable).
CC       Reduction of this diene intermediate could give the known metabolite
CC       piliformic acid (Probable). On the pathway to oryzine B and oryzine A,
CC       however, hydroxylation of the diene by the alpha-ketoglutarate-
CC       dependent dioxygenase oryG and lactonisation by the lactonohydrolases
CC       oryH or oryL could give oryzine B directly (Probable). Finally, enoyl
CC       reduction by the dehydrogenase oryD would then convert oryzine B into
CC       oryzine A (Probable). {ECO:0000269|PubMed:30104550,
CC       ECO:0000305|PubMed:30104550}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000305|PubMed:30104550}.
CC   -!- SIMILARITY: Belongs to the oryJ family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAE66068.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP007175; BAE66068.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_001827201.2; XM_001827149.2.
DR   AlphaFoldDB; Q2TXF5; -.
DR   SMR; Q2TXF5; -.
DR   EnsemblFungi; BAE66068; BAE66068; AO090010000166.
DR   HOGENOM; CLU_096188_0_1_1; -.
DR   Proteomes; UP000006564; Chromosome 8.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR013096; Cupin_2.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF07883; Cupin_2; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
PE   3: Inferred from homology;
KW   Reference proteome.
FT   CHAIN           1..179
FT                   /note="Oryzines biosynthesis cluster protein J"
FT                   /id="PRO_0000450499"
SQ   SEQUENCE   179 AA;  20206 MW;  82C28F925CA63891 CRC64;
     MGSLPEKDFP QVHRFITTHK EDGTPTFETK IPEPIEWERT NIGVDFFLAY TLGSFPAPLS
     HDADLNQYKD HLVNHPPFMI PGGAVVRYVD YHPGCEPMWH RTVTVDFGVV IEGELELEVE
     GGEKRLMKRG DVAVQRGTNH CWRNPSKTQF ARALYIALDA KPVIVNGQEL GESLGEVKH