OS25_PLAFO
ID OS25_PLAFO Reviewed; 217 AA.
AC P13829;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=25 kDa ookinete surface antigen;
DE AltName: Full=Pfs25;
DE Flags: Precursor;
OS Plasmodium falciparum (isolate NF54).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=5843;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX PubMed=3283563; DOI=10.1038/333074a0;
RA Kaslow D.C., Quakyi I.A., Syin C., Raum M.G., Keister D.B., Coligan J.E.,
RA McCutchan T.F., Miller L.H.;
RT "A vaccine candidate from the sexual stage of human malaria that contains
RT EGF-like domains.";
RL Nature 333:74-76(1988).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed on zygotes and ookinetes.
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DR EMBL; X07802; CAA30646.1; -; Genomic_DNA.
DR PIR; S00769; S00769.
DR PDB; 6B08; X-ray; 2.20 A; A=20-193.
DR PDB; 6B0A; X-ray; 2.50 A; A=22-193.
DR PDB; 6B0E; X-ray; 3.30 A; E=22-193.
DR PDB; 6B0G; X-ray; 1.90 A; E=22-193.
DR PDB; 6B0H; X-ray; 2.70 A; I/J=20-193.
DR PDB; 6PHB; X-ray; 2.00 A; E/I=22-193.
DR PDB; 6PHC; X-ray; 2.90 A; E/I=22-193.
DR PDB; 6PHD; X-ray; 3.10 A; C=22-193.
DR PDB; 6PHF; X-ray; 3.10 A; E/G=22-193.
DR PDBsum; 6B08; -.
DR PDBsum; 6B0A; -.
DR PDBsum; 6B0E; -.
DR PDBsum; 6B0G; -.
DR PDBsum; 6B0H; -.
DR PDBsum; 6PHB; -.
DR PDBsum; 6PHC; -.
DR PDBsum; 6PHD; -.
DR PDBsum; 6PHF; -.
DR AlphaFoldDB; P13829; -.
DR SMR; P13829; -.
DR PRIDE; P13829; -.
DR ABCD; P13829; 12 sequenced antibodies.
DR VEuPathDB; PlasmoDB:PfNF54_100035800; -.
DR OMA; TCSPCDF; -.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009986; C:cell surface; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR010423; Pvs25/Psv28_EGF.
DR Pfam; PF06247; Plasmod_Pvs28; 4.
DR SMART; SM00181; EGF; 3.
DR PROSITE; PS01186; EGF_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Direct protein sequencing; Disulfide bond;
KW EGF-like domain; Glycoprotein; GPI-anchor; Lipoprotein; Malaria; Membrane;
KW Repeat; Signal.
FT SIGNAL 1..16
FT CHAIN 17..196
FT /note="25 kDa ookinete surface antigen"
FT /id="PRO_0000024567"
FT PROPEP 197..217
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000024568"
FT DOMAIN 30..59
FT /note="EGF-like 1; truncated"
FT DOMAIN 61..106
FT /note="EGF-like 2"
FT DOMAIN 106..150
FT /note="EGF-like 3"
FT DOMAIN 153..193
FT /note="EGF-like 4"
FT LIPID 196
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 112
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 165
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 187
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 202
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 65..80
FT /evidence="ECO:0000250"
FT DISULFID 74..92
FT /evidence="ECO:0000250"
FT DISULFID 94..105
FT /evidence="ECO:0000250"
FT DISULFID 110..120
FT /evidence="ECO:0000250"
FT DISULFID 115..133
FT /evidence="ECO:0000250"
FT DISULFID 135..149
FT /evidence="ECO:0000250"
FT DISULFID 157..168
FT /evidence="ECO:0000250"
FT DISULFID 161..177
FT /evidence="ECO:0000250"
FT DISULFID 179..192
FT /evidence="ECO:0000250"
FT STRAND 33..37
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 42..47
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 51..54
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 57..59
FT /evidence="ECO:0007829|PDB:6B0G"
FT TURN 68..71
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 72..75
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 78..83
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 85..87
FT /evidence="ECO:0007829|PDB:6PHB"
FT STRAND 89..94
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 98..101
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 104..107
FT /evidence="ECO:0007829|PDB:6B0G"
FT HELIX 108..110
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 116..122
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 125..128
FT /evidence="ECO:0007829|PDB:6B08"
FT STRAND 131..135
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:6B0G"
FT HELIX 143..145
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 151..153
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 166..171
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 174..179
FT /evidence="ECO:0007829|PDB:6B0G"
FT STRAND 183..185
FT /evidence="ECO:0007829|PDB:6B08"
FT TURN 187..189
FT /evidence="ECO:0007829|PDB:6B08"
FT STRAND 190..193
FT /evidence="ECO:0007829|PDB:6B08"
SQ SEQUENCE 217 AA; 24143 MW; 19DC1F4CED7CF19F CRC64;
MNKLYSLFLF LFIQLSIKYN NAKVTVDTVC KRGFLIQMSG HLECKCENDL VLVNEETCEE
KVLKCDEKTV NKPCGDFSKC IKIDGNPVSY ACKCNLGYDM VNNVCIPNEC KNVTCGNGKC
ILDTSNPVKT GVCSCNIGKV PNVQDQNKCS KDGETKCSLK CLKENETCKA VDGIYKCDCK
DGFIIDNESS ICTAFSAYNI LNLSIMFILF SVCFFIM
