ASB3_MOUSE
ID ASB3_MOUSE Reviewed; 525 AA.
AC Q9WV72; Q5SSV5;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Ankyrin repeat and SOCS box protein 3;
DE Short=ASB-3;
GN Name=Asb3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=11111040; DOI=10.1016/s0378-1119(00)00402-9;
RA Kile B.T., Viney E.M., Willson T.A., Brodnicki T.C., Cancilla M.R.,
RA Herlihy A.S., Croker B.A., Baca M., Nicola N.A., Hilton D.J.,
RA Alexander W.S.;
RT "Cloning and characterization of the genes encoding the ankyrin repeat and
RT SOCS box-containing proteins Asb-1, Asb-2, Asb-3 and Asb-4.";
RL Gene 258:31-41(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Probable substrate-recognition component of a SCF-like ECS
CC (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex
CC which mediates the ubiquitination and subsequent proteasomal
CC degradation of target proteins. Recognizes TNFRSF1B (By similarity).
CC {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Interacts with ELOB and TNFRSF1B. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Widely expressed; highest expression in testis and
CC spleen. {ECO:0000269|PubMed:11111040}.
CC -!- DOMAIN: The SOCS box domain mediates the interaction with the Elongin
CC BC complex, an adapter module in different E3 ubiquitin-protein ligase
CC complexes. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ankyrin SOCS box (ASB) family.
CC {ECO:0000305}.
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DR EMBL; AF155354; AAD38810.1; -; mRNA.
DR EMBL; AK143580; BAE25448.1; -; mRNA.
DR EMBL; AL646095; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL662891; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL732621; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS24511.1; -.
DR RefSeq; NP_076395.2; NM_023906.3.
DR RefSeq; XP_006514834.1; XM_006514771.3.
DR AlphaFoldDB; Q9WV72; -.
DR SMR; Q9WV72; -.
DR BioGRID; 211146; 2.
DR IntAct; Q9WV72; 2.
DR STRING; 10090.ENSMUSP00000020551; -.
DR iPTMnet; Q9WV72; -.
DR PhosphoSitePlus; Q9WV72; -.
DR MaxQB; Q9WV72; -.
DR PaxDb; Q9WV72; -.
DR PRIDE; Q9WV72; -.
DR ProteomicsDB; 277042; -.
DR Antibodypedia; 1141; 163 antibodies from 22 providers.
DR DNASU; 65257; -.
DR Ensembl; ENSMUST00000020551; ENSMUSP00000020551; ENSMUSG00000020305.
DR GeneID; 65257; -.
DR KEGG; mmu:65257; -.
DR UCSC; uc007iif.1; mouse.
DR CTD; 51130; -.
DR MGI; MGI:1929749; Asb3.
DR VEuPathDB; HostDB:ENSMUSG00000020305; -.
DR eggNOG; KOG0504; Eukaryota.
DR GeneTree; ENSGT00940000159080; -.
DR InParanoid; Q9WV72; -.
DR OrthoDB; 1392423at2759; -.
DR PhylomeDB; Q9WV72; -.
DR TreeFam; TF315127; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 65257; 4 hits in 71 CRISPR screens.
DR ChiTaRS; Asb3; mouse.
DR PRO; PR:Q9WV72; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9WV72; protein.
DR Bgee; ENSMUSG00000020305; Expressed in spermatocyte and 250 other tissues.
DR ExpressionAtlas; Q9WV72; baseline and differential.
DR Genevisible; Q9WV72; MM.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd03722; SOCS_ASB3; 1.
DR Gene3D; 1.25.40.20; -; 2.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR037329; ASB3_SOCS.
DR InterPro; IPR001496; SOCS_box.
DR InterPro; IPR036036; SOCS_box-like_dom_sf.
DR Pfam; PF12796; Ank_2; 2.
DR Pfam; PF13606; Ank_3; 1.
DR Pfam; PF13637; Ank_4; 1.
DR Pfam; PF07525; SOCS_box; 1.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 10.
DR SMART; SM00969; SOCS_box; 1.
DR SUPFAM; SSF158235; SSF158235; 1.
DR SUPFAM; SSF48403; SSF48403; 2.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 6.
DR PROSITE; PS50225; SOCS; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..525
FT /note="Ankyrin repeat and SOCS box protein 3"
FT /id="PRO_0000066927"
FT REPEAT 9..38
FT /note="ANK 1"
FT REPEAT 42..71
FT /note="ANK 2"
FT REPEAT 78..107
FT /note="ANK 3"
FT REPEAT 111..140
FT /note="ANK 4"
FT REPEAT 145..174
FT /note="ANK 5"
FT REPEAT 178..207
FT /note="ANK 6"
FT REPEAT 211..240
FT /note="ANK 7"
FT REPEAT 246..275
FT /note="ANK 8"
FT REPEAT 279..308
FT /note="ANK 9"
FT REPEAT 315..346
FT /note="ANK 10"
FT DOMAIN 442..505
FT /note="SOCS box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00194"
FT CONFLICT 163
FT /note="R -> K (in Ref. 1; AAD38810)"
FT /evidence="ECO:0000305"
FT CONFLICT 277
FT /note="G -> E (in Ref. 1; AAD38810)"
FT /evidence="ECO:0000305"
FT CONFLICT 418
FT /note="A -> V (in Ref. 1; AAD38810)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 525 AA; 58283 MW; DEFCAC73EE2AF5E7 CRC64;
MDFTEAYSDT CSTVGLAARE GNVKILRKLL KKGRSVDVAD NRGWMPIHEA AYHNAVECLQ
MLIHTDSSEN YIKAKTFEGF CALHLAVSQG HWKITQILLE AGADPNETTL EETTPLFLAV
ESGRIDVLKL LLQHGANVNG SHSMSGWNSL HQASFQGNAE TIRLLLKQGA DRECQDDFGI
TPLFVAAQYG KLESMSILIS SGANVNCQAL DKATPLFIAA QEGHTKCVEL LLSSGADPDL
YCNEDNWQLP IHAAAQMGHT ETLDLLIPRT NRACDTGPDK VSPVYSAVFG GREECLEMLL
QNGYSPDAQM CLVFGFSSPL CMAFQKDCDF SGIVNILLKY GAQLNELHLA YCLKYEKFSM
FCSFLKKGSL MVPWNHTSEF ISYAVKAQTK YKAWLPHLLL AGFDPLILLC SSSWVDSASD
DILIFTLEFS NWKRLPSAVE KMLSVRANSS WALQQHLASV PSLTHLCRLE IRASLKAEHL
HSDIFIHQLP LPRSLQNYLL YEEVLRMNEI LEPAANQDGE TSKAT
