ID   OS28_PLAGA              Reviewed;         222 AA.
AC   Q05439;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=28 kDa ookinete surface antigen;
DE   AltName: Full=Pgs28;
DE   Flags: Precursor;
OS   Plasmodium gallinaceum.
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodiidae; Plasmodium; Plasmodium (Haemamoeba).
OX   NCBI_TaxID=5849;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8426118; DOI=10.1084/jem.177.2.505;
RA   Duffy P.E., Kaslow D.C., Pimenta P.;
RT   "Pgs28 belongs to a family of epidermal growth factor-like antigens that
RT   are targets of malaria transmission-blocking antibodies.";
RL   J. Exp. Med. 177:505-510(1993).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC       anchor {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Expressed on zygotes and ookinetes.
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DR   EMBL; M96886; AAA29726.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q05439; -.
DR   SMR; Q05439; -.
DR   VEuPathDB; PlasmoDB:PGAL8A_00490200; -.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000742; EGF-like_dom.
DR   SMART; SM00181; EGF; 2.
DR   PROSITE; PS01186; EGF_2; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Disulfide bond; EGF-like domain; Glycoprotein; GPI-anchor;
KW   Lipoprotein; Malaria; Membrane; Repeat; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..200
FT                   /note="28 kDa ookinete surface antigen"
FT                   /id="PRO_0000024573"
FT   PROPEP          201..222
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000024574"
FT   DOMAIN          29..58
FT                   /note="EGF-like 1; truncated"
FT   DOMAIN          59..107
FT                   /note="EGF-like 2"
FT   DOMAIN          107..150
FT                   /note="EGF-like 3"
FT   DOMAIN          151..176
FT                   /note="EGF-like 4; truncated"
FT   LIPID           200
FT                   /note="GPI-anchor amidated glycine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        87
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        63..79
FT                   /evidence="ECO:0000250"
FT   DISULFID        73..93
FT                   /evidence="ECO:0000250"
FT   DISULFID        95..106
FT                   /evidence="ECO:0000250"
FT   DISULFID        111..121
FT                   /evidence="ECO:0000250"
FT   DISULFID        116..133
FT                   /evidence="ECO:0000250"
FT   DISULFID        135..149
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   222 AA;  24543 MW;  390234FD8AC82578 CRC64;
     MKIPSLYFFF FIQIAIILTI AAPSDDEPCK NGYLIEMSNH IECKCNNDYV LTNRYECEPK
     NKCTSLEDTN KPCADYARCL EDPYKDNKSN FYCLCNRGYI QYEDKCIQAE CNYKECGEGK
     CVWDGIHEDG AFCSCNIGKV INPEDNNKCT KDGDTKCTLE CAQGKKCIKH DVYYMCGNDN
     SGSGSGGGGG GGNSPPPSSG NSTLSLFNAL NIVFLIAVIY II