OSB1_ARATH
ID OSB1_ARATH Reviewed; 261 AA.
AC Q9SX99; Q9FZE9;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Protein OSB1, mitochondrial;
DE AltName: Full=Organellar single-stranded DNA-binding protein 1;
DE Flags: Precursor;
GN Name=OSB1; OrderedLocusNames=At1g47720; ORFNames=F16N3.2, T2E6.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP FUNCTION, MUTAGENESIS OF 199-TRP--ASP-201; 213-ASP-PHE-214 AND
RP 223-LEU-TRP-224, DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=17189341; DOI=10.1105/tpc.106.042028;
RA Zaegel V., Guermann B., Le Ret M., Andres C., Meyer D., Erhardt M.,
RA Canaday J., Gualberto J.M., Imbault P.;
RT "The plant-specific ssDNA binding protein OSB1 is involved in the
RT stoichiometric transmission of mitochondrial DNA in Arabidopsis.";
RL Plant Cell 18:3548-3563(2006).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND CLEAVAGE OF TRANSIT PEPTIDE AFTER
RP PHE-28.
RX PubMed=25732537; DOI=10.1093/jxb/erv064;
RA Carrie C., Venne A.S., Zahedi R.P., Soll J.;
RT "Identification of cleavage sites and substrate proteins for two
RT mitochondrial intermediate peptidases in Arabidopsis thaliana.";
RL J. Exp. Bot. 66:2691-2708(2015).
CC -!- FUNCTION: Regulates mitochondrial DNA recombination. Represses
CC homologous recombination, preventing mitochondrial genome instability
CC and unbalanced transmission of alternative mtDNA configurations. Binds
CC preferentially single-stranded DNA. Does not bind to RNA.
CC {ECO:0000269|PubMed:17189341}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:17189341,
CC ECO:0000305|PubMed:25732537}.
CC -!- TISSUE SPECIFICITY: Expressed in root elongation zone and in
CC gametophytic cells. {ECO:0000269|PubMed:17189341}.
CC -!- DOMAIN: The PDF region is required for protein-DNA interaction while
CC the SSB domain is not required for ssDNA binding.
CC -!- DISRUPTION PHENOTYPE: Severe leaf variegation, distortion and partial
CC sterility. {ECO:0000269|PubMed:17189341}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF99794.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007519; AAD46017.1; -; Genomic_DNA.
DR EMBL; AC012463; AAF99794.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32204.1; -; Genomic_DNA.
DR EMBL; BT005269; AAO63333.1; -; mRNA.
DR EMBL; AK119114; BAC43686.1; -; mRNA.
DR RefSeq; NP_175203.2; NM_103665.3.
DR AlphaFoldDB; Q9SX99; -.
DR SMR; Q9SX99; -.
DR STRING; 3702.AT1G47720.1; -.
DR PaxDb; Q9SX99; -.
DR PRIDE; Q9SX99; -.
DR ProteomicsDB; 248910; -.
DR EnsemblPlants; AT1G47720.1; AT1G47720.1; AT1G47720.
DR GeneID; 841183; -.
DR Gramene; AT1G47720.1; AT1G47720.1; AT1G47720.
DR KEGG; ath:AT1G47720; -.
DR Araport; AT1G47720; -.
DR TAIR; locus:2015353; AT1G47720.
DR eggNOG; ENOG502S048; Eukaryota.
DR HOGENOM; CLU_049248_0_0_1; -.
DR InParanoid; Q9SX99; -.
DR OMA; DWWDNRR; -.
DR OrthoDB; 1476527at2759; -.
DR PhylomeDB; Q9SX99; -.
DR PRO; PR:Q9SX99; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SX99; baseline and differential.
DR Genevisible; Q9SX99; AT.
DR GO; GO:0048046; C:apoplast; HDA:TAIR.
DR GO; GO:0042645; C:mitochondrial nucleoid; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0009295; C:nucleoid; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IDA:TAIR.
DR GO; GO:0006264; P:mitochondrial DNA replication; IBA:GO_Central.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:TAIR.
DR GO; GO:0045910; P:negative regulation of DNA recombination; IMP:TAIR.
DR GO; GO:0051096; P:positive regulation of helicase activity; IBA:GO_Central.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR000424; Primosome_PriB/ssb.
DR InterPro; IPR011344; ssDNA-bd.
DR PANTHER; PTHR10302; PTHR10302; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR PROSITE; PS50935; SSB; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Mitochondrion; Reference proteome; Transit peptide.
FT TRANSIT 1..28
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:25732537"
FT CHAIN 29..261
FT /note="Protein OSB1, mitochondrial"
FT /id="PRO_0000383608"
FT DOMAIN 55..155
FT /note="SSB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00252"
FT REGION 189..238
FT /note="PDF region"
FT MUTAGEN 199..201
FT /note="WWD->GWG: Reduced ssDNA-binding."
FT /evidence="ECO:0000269|PubMed:17189341"
FT MUTAGEN 213..214
FT /note="DF->GG: Reduced ssDNA-binding."
FT /evidence="ECO:0000269|PubMed:17189341"
FT MUTAGEN 223..224
FT /note="LW->RG: Reduced ssDNA-binding."
FT /evidence="ECO:0000269|PubMed:17189341"
SQ SEQUENCE 261 AA; 30564 MW; E5FA365CC5844E97 CRC64;
MNTFFKLGSL IQRTASQISS SFPKSRFFSD GESAVYHHAR LFKKPLSTKL KFNLVNSVSL
MGFVDRSIQV MNTGPDRFGV FTILRVKDPL NPNRSFRISL RMWDAMARTC IAHLKLNDHI
LVSGRLESYS KSSSDVYSGL NLDYQVKVAE VNYVAAPPSH VLDSQISKNP KTKTEDDIEE
SKKDEIYLWQ VFFSNPYDWW DNRRNKKNPK QPDFKHKDTG EALWLCSDLP DWITRRLELF
DQKNRFYDEE KTRRDRLSDY I
