ID   OSB1_DICDI              Reviewed;         389 AA.
AC   Q8MPQ7; Q54VW8;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Oxysterol-binding protein 1;
DE   AltName: Full=OSBPa;
GN   Name=osbA; ORFNames=DDB_G0280053;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION
RP   WITH DSTC, AND DEVELOPMENTAL STAGE.
RC   STRAIN=AX2;
RX   PubMed=12220630; DOI=10.1016/s0014-5793(02)03150-2;
RA   Fukuzawa M., Williams J.G.;
RT   "OSBPa, a predicted oxysterol binding protein of Dictyostelium, is required
RT   for regulated entry into culmination.";
RL   FEBS Lett. 527:37-42(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: May play a role in the regulation of the slug-fruiting body
CC       switch. {ECO:0000269|PubMed:12220630}.
CC   -!- SUBUNIT: Interacts with dstC. {ECO:0000269|PubMed:12220630}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12220630}.
CC       Note=Localizes predominantly to the cell periphery, but is also found
CC       in the perinuclear region in some cells.
CC   -!- DEVELOPMENTAL STAGE: Developmentally regulated with a peak during
CC       culmination. Slightly enriched in pstO and rearguard cells.
CC       {ECO:0000269|PubMed:12220630}.
CC   -!- SIMILARITY: Belongs to the OSBP family. {ECO:0000305}.
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DR   EMBL; AJ488770; CAD32824.1; -; mRNA.
DR   EMBL; AAFI02000035; EAL67253.1; -; Genomic_DNA.
DR   RefSeq; XP_641237.1; XM_636145.1.
DR   AlphaFoldDB; Q8MPQ7; -.
DR   SMR; Q8MPQ7; -.
DR   STRING; 44689.DDB0214942; -.
DR   PaxDb; Q8MPQ7; -.
DR   EnsemblProtists; EAL67253; EAL67253; DDB_G0280053.
DR   GeneID; 8622368; -.
DR   KEGG; ddi:DDB_G0280053; -.
DR   dictyBase; DDB_G0280053; osbA.
DR   eggNOG; KOG2210; Eukaryota.
DR   HOGENOM; CLU_706815_0_0_1; -.
DR   InParanoid; Q8MPQ7; -.
DR   OMA; PAAWACH; -.
DR   PhylomeDB; Q8MPQ7; -.
DR   Reactome; R-DDI-1482801; Acyl chain remodelling of PS.
DR   Reactome; R-DDI-192105; Synthesis of bile acids and bile salts.
DR   Reactome; R-DDI-9013407; RHOH GTPase cycle.
DR   PRO; PR:Q8MPQ7; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0071944; C:cell periphery; IDA:dictyBase.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:dictyBase.
DR   GO; GO:0032934; F:sterol binding; IBA:GO_Central.
DR   GO; GO:0015248; F:sterol transporter activity; IBA:GO_Central.
DR   GO; GO:0031154; P:culmination involved in sorocarp development; IMP:dictyBase.
DR   InterPro; IPR037239; OSBP_sf.
DR   InterPro; IPR000648; Oxysterol-bd.
DR   PANTHER; PTHR10972; PTHR10972; 1.
DR   Pfam; PF01237; Oxysterol_BP; 1.
DR   SUPFAM; SSF144000; SSF144000; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Reference proteome.
FT   CHAIN           1..389
FT                   /note="Oxysterol-binding protein 1"
FT                   /id="PRO_0000328466"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          1..31
FT                   /evidence="ECO:0000255"
FT   COILED          340..371
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   389 AA;  44110 MW;  AB37133034FEA350 CRC64;
     MGKKDKNVSV EEEVDEAEIE KLAAENANKP APQLTKEDLD AMDDAEPGTS RLALIGKMIK
     KVSIGTDISN ISMPGSFILA KSTLSYFSDN FSSFFGELLK ANKIDNELER MLQVQRYLFT
     TLKETEDTTR KPLNPILGET WQANIHVKDG DGNDVTDNYF FAEQISHHPP ISSSTVYNKK
     EGVNCTFCLP VRSQFMGTYV KISFEGESHI TFEKYDEKFT FVAPPMAIRI FRSFSEYVGK
     GILKSDKNDY LIKSTYTSKP LFGGVYNGFE SKVYKGKEKL YKIKGTWSGD MKITNLKTNE
     TTPFFTRPTE SAKVVFPDDG NTLPTDSSVV WKGVFDASKK DDVKGMSQEK VKVEEEQRKL
     AHHRKNADEW KPVHFNKIDG RWQLVNLKD