OSB3_ARATH
ID OSB3_ARATH Reviewed; 440 AA.
AC Q8GWJ4; O48581; Q8LFC9;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Protein OSB3, chloroplastic/mitochondrial;
DE AltName: Full=Organellar single-stranded DNA-binding protein 3;
DE Flags: Precursor;
GN Name=OSB3; OrderedLocusNames=At5g44785; ORFNames=K23L20.13, T19K24.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=16990374; DOI=10.1093/jxb/erl130;
RA Butenko M.A., Stenvik G.-E., Alm V., Saether B., Patterson S.E.,
RA Aalen R.B.;
RT "Ethylene-dependent and -independent pathways controlling floral abscission
RT are revealed to converge using promoter::reporter gene constructs in the
RT ida abscission mutant.";
RL J. Exp. Bot. 57:3627-3637(2006).
RN [8]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=17189341; DOI=10.1105/tpc.106.042028;
RA Zaegel V., Guermann B., Le Ret M., Andres C., Meyer D., Erhardt M.,
RA Canaday J., Gualberto J.M., Imbault P.;
RT "The plant-specific ssDNA binding protein OSB1 is involved in the
RT stoichiometric transmission of mitochondrial DNA in Arabidopsis.";
RL Plant Cell 18:3548-3563(2006).
CC -!- FUNCTION: Binds single-stranded DNA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:17189341}.
CC Plastid, chloroplast {ECO:0000269|PubMed:17189341}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8GWJ4-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed primarily in the female gametophyte and
CC in the floral abscission zone. {ECO:0000269|PubMed:16990374,
CC ECO:0000269|PubMed:17189341}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:17189341}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC79142.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002342; AAC79142.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95162.1; -; Genomic_DNA.
DR EMBL; AK118806; BAC43396.1; -; mRNA.
DR EMBL; AY084919; AAM61481.1; -; mRNA.
DR EMBL; BT005411; AAO63831.1; -; mRNA.
DR EMBL; BT006477; AAP21285.1; -; mRNA.
DR EMBL; AK227530; BAE99530.1; -; mRNA.
DR RefSeq; NP_568639.1; NM_123846.4. [Q8GWJ4-1]
DR AlphaFoldDB; Q8GWJ4; -.
DR SMR; Q8GWJ4; -.
DR STRING; 3702.AT5G44785.2; -.
DR PaxDb; Q8GWJ4; -.
DR PRIDE; Q8GWJ4; -.
DR ProteomicsDB; 248669; -. [Q8GWJ4-1]
DR EnsemblPlants; AT5G44785.1; AT5G44785.1; AT5G44785. [Q8GWJ4-1]
DR GeneID; 834508; -.
DR Gramene; AT5G44785.1; AT5G44785.1; AT5G44785. [Q8GWJ4-1]
DR KEGG; ath:AT5G44785; -.
DR Araport; AT5G44785; -.
DR eggNOG; ENOG502S0Q4; Eukaryota.
DR InParanoid; Q8GWJ4; -.
DR OMA; WVISHQI; -.
DR PhylomeDB; Q8GWJ4; -.
DR PRO; PR:Q8GWJ4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8GWJ4; baseline and differential.
DR Genevisible; Q8GWJ4; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0042645; C:mitochondrial nucleoid; IBA:GO_Central.
DR GO; GO:0009295; C:nucleoid; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006264; P:mitochondrial DNA replication; IBA:GO_Central.
DR GO; GO:0051096; P:positive regulation of helicase activity; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR000424; Primosome_PriB/ssb.
DR InterPro; IPR011344; ssDNA-bd.
DR PANTHER; PTHR10302; PTHR10302; 4.
DR SUPFAM; SSF50249; SSF50249; 1.
DR PROSITE; PS50935; SSB; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; DNA-binding; Mitochondrion; Plastid;
KW Reference proteome; Repeat; Transit peptide.
FT TRANSIT 1..61
FT /note="Chloroplast and mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 62..440
FT /note="Protein OSB3, chloroplastic/mitochondrial"
FT /id="PRO_0000383610"
FT DOMAIN 80..178
FT /note="SSB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00252"
FT REGION 218..270
FT /note="PDF region 1"
FT REGION 294..342
FT /note="PDF region 2"
FT REGION 380..428
FT /note="PDF region 3"
FT CONFLICT 73
FT /note="K -> T (in Ref. 4; AAM61481)"
FT /evidence="ECO:0000305"
FT CONFLICT 352
FT /note="F -> L (in Ref. 4; AAM61481)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 440 AA; 49900 MW; FF59126173F428A9 CRC64;
MNLISRTLTR AVSSSLYHSK AAKLPTQKWI ISQQIRVFSA TVISGGGKKP LAKVSVKPPL
NVATEKESTP PKKIEYKPEI SNWINLIGFV EQPVQFGPCS DGKFWAGTVI SQRSGSKSSN
FWIPIIFEGD LAKIAVQHVK KEDRIHVSGK LFIDSPPPNV TYSQSNVQVM VQNLNFVQAA
TSTTKTISPP EKEVTSIKKK PARSKKVKVI DEETSNSWKH LIENPKEWLD HRGNKANGLV
KPGHPDFKMK VGGLSLWLST APDWALLKLE ELKFDVLVPK GNIKLNQLKG EESWKDLVQN
PDKWLDNRSD KTNVKYPDFK HKETGEALWM TNSPIWVLSK LPPLKKNQER PFMSNKVSQL
ELDVEVPKGN LKQLKREEIW KNLVENPSKW WDNRLDKRNP KGPDFKHKET GEALWIGDSP
TWALSKLPPL KKNQERPVMA
