OSB4_ARATH
ID OSB4_ARATH Reviewed; 360 AA.
AC Q9FYJ2; Q8LCI8;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Protein OSB4, chloroplastic;
DE AltName: Full=Organellar single-stranded DNA-binding protein 4;
DE Flags: Precursor;
GN Name=OSB4; OrderedLocusNames=At1g31010; ORFNames=F17F8.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION.
RX PubMed=17189341; DOI=10.1105/tpc.106.042028;
RA Zaegel V., Guermann B., Le Ret M., Andres C., Meyer D., Erhardt M.,
RA Canaday J., Gualberto J.M., Imbault P.;
RT "The plant-specific ssDNA binding protein OSB1 is involved in the
RT stoichiometric transmission of mitochondrial DNA in Arabidopsis.";
RL Plant Cell 18:3548-3563(2006).
CC -!- FUNCTION: Binds single-stranded DNA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
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DR EMBL; AC000107; AAF98182.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31306.1; -; Genomic_DNA.
DR EMBL; AY086580; AAM63642.1; -; mRNA.
DR PIR; G86435; G86435.
DR RefSeq; NP_564370.1; NM_102839.5.
DR AlphaFoldDB; Q9FYJ2; -.
DR SMR; Q9FYJ2; -.
DR STRING; 3702.AT1G31010.1; -.
DR iPTMnet; Q9FYJ2; -.
DR PaxDb; Q9FYJ2; -.
DR PRIDE; Q9FYJ2; -.
DR ProteomicsDB; 248829; -.
DR EnsemblPlants; AT1G31010.1; AT1G31010.1; AT1G31010.
DR GeneID; 839987; -.
DR Gramene; AT1G31010.1; AT1G31010.1; AT1G31010.
DR KEGG; ath:AT1G31010; -.
DR Araport; AT1G31010; -.
DR TAIR; locus:2015721; AT1G31010.
DR eggNOG; ENOG502QPSC; Eukaryota.
DR HOGENOM; CLU_035942_0_0_1; -.
DR InParanoid; Q9FYJ2; -.
DR OMA; MQFLARS; -.
DR OrthoDB; 1476527at2759; -.
DR PhylomeDB; Q9FYJ2; -.
DR PRO; PR:Q9FYJ2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FYJ2; baseline and differential.
DR Genevisible; Q9FYJ2; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0042645; C:mitochondrial nucleoid; IBA:GO_Central.
DR GO; GO:0009295; C:nucleoid; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006264; P:mitochondrial DNA replication; IBA:GO_Central.
DR GO; GO:0051096; P:positive regulation of helicase activity; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR000424; Primosome_PriB/ssb.
DR InterPro; IPR011344; ssDNA-bd.
DR PANTHER; PTHR10302; PTHR10302; 1.
DR Pfam; PF00436; SSB; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR PROSITE; PS50935; SSB; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; DNA-binding; Plastid; Reference proteome; Repeat;
KW Transit peptide.
FT TRANSIT 1..61
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 62..360
FT /note="Protein OSB4, chloroplastic"
FT /id="PRO_0000383611"
FT DOMAIN 71..188
FT /note="SSB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00252"
FT REGION 28..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 224..276
FT /note="PDF region 1"
FT REGION 296..344
FT /note="PDF region 2"
FT COMPBIAS 28..46
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 255
FT /note="K -> M (in Ref. 3; AAM63642)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 360 AA; 40627 MW; 5D0CDD4633460607 CRC64;
MQFLGRSISK SIRPSLNSTA RKSWVLSSQQ FLSTSSTESS SRTRGGGGGN RAEKSSEEWP
RPMEVPYQPK IANSIDLIGY VHQPVQFDST LDGKFWAGTV ISHEPSSDSK SESDSSSNFW
IPVLFEGDLA HTANSYLKKN DRVHITGQIL GDVIQSGANS DQAHVQLFKS FHGSFSHQVM
VRDLHYIEGS KAMPKVLPTL DQNEGVLKHS ASVQRGREFG TNLWFDLVDK PNEWCDYREM
KQNGSVNPKH PDFKKKDGSQ ALWLNNAPTE ILSELKDVKF DIPKYAKQPK AGEESWKDLV
DNMNKWWDNR VDKRTPKSPD FKHKETGVGL WLSDSPSWVL EKLPPPKSKT SDIYGVQEMF
