OSBL9_MOUSE
ID OSBL9_MOUSE Reviewed; 736 AA.
AC A2A8Z1; A2A9Q8;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Oxysterol-binding protein-related protein 9;
DE Short=ORP-9;
DE Short=OSBP-related protein 9;
GN Name=Osbpl9; Synonyms=Orp9;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: Heterodimer with OSBPL11. Interacts with OSBPL10.
CC {ECO:0000250|UniProtKB:Q96SU4}.
CC -!- SUBCELLULAR LOCATION: Late endosome membrane {ECO:0000250}. Golgi
CC apparatus, trans-Golgi network membrane {ECO:0000250}. Note=Localizes
CC at the Golgi-late endosome interface. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A2A8Z1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2A8Z1-2; Sequence=VSP_036572, VSP_036573;
CC -!- SIMILARITY: Belongs to the OSBP family. {ECO:0000305}.
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DR EMBL; AL627103; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL627406; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS51258.1; -. [A2A8Z1-2]
DR CCDS; CCDS89802.1; -. [A2A8Z1-1]
DR RefSeq; NP_775485.2; NM_173350.3. [A2A8Z1-2]
DR RefSeq; XP_006502682.2; XM_006502619.3.
DR AlphaFoldDB; A2A8Z1; -.
DR SMR; A2A8Z1; -.
DR STRING; 10090.ENSMUSP00000124370; -.
DR iPTMnet; A2A8Z1; -.
DR PhosphoSitePlus; A2A8Z1; -.
DR EPD; A2A8Z1; -.
DR jPOST; A2A8Z1; -.
DR MaxQB; A2A8Z1; -.
DR PaxDb; A2A8Z1; -.
DR PeptideAtlas; A2A8Z1; -.
DR PRIDE; A2A8Z1; -.
DR ProteomicsDB; 294116; -. [A2A8Z1-1]
DR ProteomicsDB; 294117; -. [A2A8Z1-2]
DR Antibodypedia; 18985; 141 antibodies from 33 providers.
DR DNASU; 100273; -.
DR Ensembl; ENSMUST00000030288; ENSMUSP00000030288; ENSMUSG00000028559. [A2A8Z1-1]
DR Ensembl; ENSMUST00000084366; ENSMUSP00000081396; ENSMUSG00000028559. [A2A8Z1-2]
DR GeneID; 100273; -.
DR KEGG; mmu:100273; -.
DR UCSC; uc008uca.2; mouse. [A2A8Z1-2]
DR CTD; 114883; -.
DR MGI; MGI:1923784; Osbpl9.
DR VEuPathDB; HostDB:ENSMUSG00000028559; -.
DR eggNOG; KOG2210; Eukaryota.
DR GeneTree; ENSGT00940000154690; -.
DR InParanoid; A2A8Z1; -.
DR OrthoDB; 949920at2759; -.
DR PhylomeDB; A2A8Z1; -.
DR Reactome; R-MMU-192105; Synthesis of bile acids and bile salts.
DR BioGRID-ORCS; 100273; 8 hits in 75 CRISPR screens.
DR ChiTaRS; Osbpl9; mouse.
DR PRO; PR:A2A8Z1; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; A2A8Z1; protein.
DR Bgee; ENSMUSG00000028559; Expressed in stroma of bone marrow and 247 other tissues.
DR ExpressionAtlas; A2A8Z1; baseline and differential.
DR Genevisible; A2A8Z1; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0032934; F:sterol binding; IBA:GO_Central.
DR GO; GO:0015248; F:sterol transporter activity; IBA:GO_Central.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR037239; OSBP_sf.
DR InterPro; IPR000648; Oxysterol-bd.
DR InterPro; IPR018494; Oxysterol-bd_CS.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR10972; PTHR10972; 2.
DR Pfam; PF01237; Oxysterol_BP; 1.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF144000; SSF144000; 1.
DR PROSITE; PS01013; OSBP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Endosome; Golgi apparatus;
KW Lipid transport; Lipid-binding; Membrane; Phosphoprotein;
KW Reference proteome; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q96SU4"
FT CHAIN 2..736
FT /note="Oxysterol-binding protein-related protein 9"
FT /id="PRO_0000366289"
FT DOMAIN 2..99
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 209..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..289
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..350
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 351..368
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q96SU4"
FT MOD_RES 306
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96SU4"
FT MOD_RES 324
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96SU4"
FT MOD_RES 325
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96SU4"
FT MOD_RES 326
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 329
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96SU4"
FT MOD_RES 611
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96SU4"
FT VAR_SEQ 1..97
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036572"
FT VAR_SEQ 98..106
FT /note="ILRHTLQLQ -> MALLLAACG (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036573"
SQ SEQUENCE 736 AA; 83109 MW; 4F033CB93DFD4305 CRC64;
MASIVEGPLS KWTNVMKGWQ YRWFVLDYNA GLLSYYTSKD KMMRGSRRGC VRLRGAVIGI
DDEDDSTFTI TVDQKTFHFQ ARDADEREKW IHALEETILR HTLQLQGLDS GFIPSVQDFD
KKLTEADAYL QILIEQLKLF DDKLQNCKDD EQRKKVETLK DTTNSMVESI KHCIVLLQIA
KDQSNAEQHA DGIISTINPV DAIYQPSPLE PVISTMPSQT ALPPEPAQLC KSEQRPSSLP
VGPVLATLGH HQTPTPNSTG SGNSPPSSSL TPPSHVNLSP NTVPEFSYSS SEDEFYDADE
FHQSGSSPKR LIDSSGSASV LTHSSSGNSL KRPDTTESLN SSMSNGTSDA DLFDSHDDRD
DDGEAGSVEE HKSVIMHLLS QVRLGMDLTK VVLPTFILER RSLLEMYADF FAHPDLFVSI
SDQKDPRDRM VQVVKWYLSA FHAGRRGSVA KKPYNPILGE IFQCHWTLPN DTEENAELVS
EGPVPWVSKN SVTFVAEQVS HHPPISAFYA ECFNKKIQFN AHIWTKSKFL GMSIGVHNIG
QGCVSCLEYD EHYILTFPNG YGRSILTVPW VELGGECNIN CSKTGYSANI VFHTKPFYGG
KKHRITAEIF SPNDKKSFCS IEGEWNGIMY AKYATGENTV FVDTKKLPII KKKVRKLEDQ
NEYESRSLWK DVTFNLKIRD IDAATEAKHR LEERQRAEAR ERKEKEIQWE TRLFHEDGEC
WVYDEPLLKR LGAVKH
