ASB6_MOUSE
ID ASB6_MOUSE Reviewed; 418 AA.
AC Q91ZU1; Q8VEC6;
DT 28-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Ankyrin repeat and SOCS box protein 6;
DE Short=ASB-6;
GN Name=Asb6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11509662; DOI=10.1128/mcb.21.18.6189-6197.2001;
RA Kile B.T., Metcalf D., Mifsud S., DiRago L., Nicola N.A., Hilton D.J.,
RA Alexander W.S.;
RT "Functional analysis of Asb-1 using genetic modification in mice.";
RL Mol. Cell. Biol. 21:6189-6197(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH APS, IDENTIFICATION IN A
RP COMPLEX CONTAINING ELOB AND ELOC, AND TISSUE SPECIFICITY.
RX PubMed=15231829; DOI=10.1074/jbc.m406101200;
RA Wilcox A., Katsanakis K.D., Bheda F., Pillay T.S.;
RT "Asb6, an adipocyte-specific ankyrin and SOCS box protein, interacts with
RT APS to enable recruitment of elongins B and C to the insulin receptor
RT signaling complex.";
RL J. Biol. Chem. 279:38881-38888(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Probable substrate-recognition component of a SCF-like ECS
CC (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex
CC which mediates the ubiquitination and subsequent proteasomal
CC degradation of target proteins. {ECO:0000250,
CC ECO:0000269|PubMed:15231829}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Binds APS. Identified in a complex with ELOB and ELOC.
CC Interacts with CUL5 and RNF7 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15231829}.
CC -!- TISSUE SPECIFICITY: Detected in adipocytes.
CC {ECO:0000269|PubMed:15231829}.
CC -!- DOMAIN: The SOCS box domain mediates the interaction with the Elongin
CC BC complex, an adapter module in different E3 ubiquitin-protein ligase
CC complexes. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ankyrin SOCS box (ASB) family.
CC {ECO:0000305}.
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DR EMBL; AF398967; AAK97489.1; -; mRNA.
DR EMBL; BC019192; AAH19192.1; -; mRNA.
DR CCDS; CCDS38096.1; -.
DR RefSeq; NP_579924.1; NM_133346.2.
DR AlphaFoldDB; Q91ZU1; -.
DR SMR; Q91ZU1; -.
DR BioGRID; 215308; 5.
DR IntAct; Q91ZU1; 2.
DR STRING; 10090.ENSMUSP00000043462; -.
DR iPTMnet; Q91ZU1; -.
DR PhosphoSitePlus; Q91ZU1; -.
DR EPD; Q91ZU1; -.
DR MaxQB; Q91ZU1; -.
DR PaxDb; Q91ZU1; -.
DR PRIDE; Q91ZU1; -.
DR ProteomicsDB; 277251; -.
DR Antibodypedia; 1154; 81 antibodies from 21 providers.
DR DNASU; 72323; -.
DR Ensembl; ENSMUST00000041726; ENSMUSP00000043462; ENSMUSG00000039483.
DR GeneID; 72323; -.
DR KEGG; mmu:72323; -.
DR UCSC; uc008jcx.1; mouse.
DR CTD; 140459; -.
DR MGI; MGI:1919573; Asb6.
DR VEuPathDB; HostDB:ENSMUSG00000039483; -.
DR eggNOG; KOG0504; Eukaryota.
DR GeneTree; ENSGT00390000006784; -.
DR HOGENOM; CLU_000134_52_2_1; -.
DR InParanoid; Q91ZU1; -.
DR OMA; HLQPWPV; -.
DR OrthoDB; 668449at2759; -.
DR PhylomeDB; Q91ZU1; -.
DR TreeFam; TF330837; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 72323; 5 hits in 72 CRISPR screens.
DR PRO; PR:Q91ZU1; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q91ZU1; protein.
DR Bgee; ENSMUSG00000039483; Expressed in embryonic brain and 223 other tissues.
DR ExpressionAtlas; Q91ZU1; baseline and differential.
DR Genevisible; Q91ZU1; MM.
DR GO; GO:0031436; C:BRCA1-BARD1 complex; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR CDD; cd03725; SOCS_ASB6; 1.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR037327; ASB6_SOCS.
DR InterPro; IPR001496; SOCS_box.
DR InterPro; IPR036036; SOCS_box-like_dom_sf.
DR Pfam; PF13857; Ank_5; 1.
DR Pfam; PF07525; SOCS_box; 1.
DR SMART; SM00248; ANK; 4.
DR SMART; SM00253; SOCS; 1.
DR SMART; SM00969; SOCS_box; 1.
DR SUPFAM; SSF158235; SSF158235; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 2.
DR PROSITE; PS50225; SOCS; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Cytoplasm; Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..418
FT /note="Ankyrin repeat and SOCS box protein 6"
FT /id="PRO_0000066934"
FT REPEAT 65..95
FT /note="ANK 1"
FT REPEAT 100..129
FT /note="ANK 2"
FT REPEAT 134..164
FT /note="ANK 3"
FT REPEAT 168..203
FT /note="ANK 4"
FT REPEAT 224..253
FT /note="ANK 5"
FT REPEAT 258..287
FT /note="ANK 6"
FT DOMAIN 358..413
FT /note="SOCS box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00194"
FT CONFLICT 189
FT /note="E -> D (in Ref. 2; AAH19192)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 418 AA; 46276 MW; 04ADE4CC0CF6E964 CRC64;
MPFLHGFRRI IFEYQPLVDA ILGALGIQDL ERQEPLDDSA SSEESRILVL TELLEQKAHS
PFYQEGVSNA LLKMAELGLT RAAAVLLQSG ANLNFEDPVT YYTALHIAVL RNQPDMVELL
VRHGADINRR DRIHESSPLD LASEEPERLP CLQRLLDLGA DVNAADKNGK TALLHALASS
DGVQIHNTEN IRLLLEGGAD VKATTKDGDT VFTCIIFLLG ETVCGDKEEA PMINRFCFQV
TQLLLAHGAD PSECPAHESL THICLKSFKL HFPLLCFLLE SGAAYNCSLH GASCWSGFNL
VFERLCSHPG CAEDDSHIEL LHKAETVLDL MVTSSQRLQL PENLNIHPVG SLAGKIQALH
ASLRQLESYP PPLKHLCRVS IRLCLRPWPV DTKVKALPLP DRLKWYLLSA HSDTQDTC
