OSM1_CAEEL
ID OSM1_CAEEL Reviewed; 1737 AA.
AC Q22830; Q1KYP6;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 4.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Intraflagellar transport protein osm-1;
DE AltName: Full=Osmotic avoidance abnormal protein 1;
GN Name=osm-1 {ECO:0000312|WormBase:T27B1.1};
GN ORFNames=T27B1.1 {ECO:0000312|WormBase:T27B1.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=16648645; DOI=10.1534/genetics.106.056721;
RA Bell L.R., Stone S., Yochem J., Shaw J.E., Herman R.K.;
RT "The molecular identities of the Caenorhabditis elegans intraflagellar
RT transport genes dyf-6, daf-10 and osm-1.";
RL Genetics 173:1275-1286(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND TISSUE
RP SPECIFICITY.
RX PubMed=10545497; DOI=10.1083/jcb.147.3.519;
RA Signor D., Wedaman K.P., Orozco J.T., Dwyer N.D., Bargmann C.I., Rose L.S.,
RA Scholey J.M.;
RT "Role of a class DHC1b dynein in retrograde transport of IFT motors and IFT
RT raft particles along cilia, but not dendrites, in chemosensory neurons of
RT living Caenorhabditis elegans.";
RL J. Cell Biol. 147:519-530(1999).
RN [4]
RP FUNCTION, IDENTIFICATION IN IFT COMPLEX B, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=28479320; DOI=10.1016/j.cub.2017.04.015;
RA Yi P., Li W.J., Dong M.Q., Ou G.;
RT "Dynein-driven retrograde intraflagellar transport is triphasic in C.
RT elegans sensory cilia.";
RL Curr. Biol. 27:1448-1461(2017).
CC -!- FUNCTION: Component of the intraflagellar transport (IFT) complex B
CC required for transport of proteins in the motile cilium
CC (PubMed:28479320). May be required for ciliary entrance and transport
CC of specific ciliary cargo proteins such as che-3 which are related to
CC motility (PubMed:28479320). Required for the maintenance and formation
CC of chemosensory cilia that detect chemosensory cues (PubMed:10545497).
CC {ECO:0000269|PubMed:10545497, ECO:0000269|PubMed:28479320}.
CC -!- SUBUNIT: Component of the IFT complex B composed of at least che-2,
CC che-13, dyf-1, dyf-3, dyf-6, dyf-11, dyf-13, ift-20, ift-74, ift-81,
CC ifta-2, osm-1, osm-5 and osm-6. {ECO:0000269|PubMed:28479320}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000269|PubMed:10545497}. Note=Emerges from the transition zones
CC and move in a bidirectional fashion along the sensory cilia, displaying
CC anterograde movement from the transition zone toward the tip of the
CC ciliary axoneme and retrograde movement from the cilium tip back toward
CC the transition zone.
CC -!- TISSUE SPECIFICITY: Expressed in amphid and phasmid chemosensory
CC neurons, where it appears to concentrate at the base of the transition
CC zones, which correspond to the basal bodies of motile and sensory
CC cilia. Moves in the retrograde direction along cilia and dendrites,
CC suggesting that it is retrieved from the distal endings of the cilia by
CC a retrograde transport pathway that moves it along cilia and then
CC dendrites, back to the neuronal cell body.
CC {ECO:0000269|PubMed:10545497}.
CC -!- DISRUPTION PHENOTYPE: Worms display defects in ciliary structure
CC resulting in defects in ability and osmotic avoidance behavior.
CC {ECO:0000269|PubMed:10545497}.
CC -!- SIMILARITY: Belongs to the IFT172 family. {ECO:0000305}.
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DR EMBL; DQ360811; ABC88648.1; -; mRNA.
DR EMBL; FO081607; CCD72791.1; -; Genomic_DNA.
DR RefSeq; NP_510681.4; NM_078280.6.
DR AlphaFoldDB; Q22830; -.
DR BioGRID; 46599; 8.
DR ComplexPortal; CPX-1290; Intraflagellar transport complex B.
DR STRING; 6239.T27B1.1; -.
DR EPD; Q22830; -.
DR PaxDb; Q22830; -.
DR PeptideAtlas; Q22830; -.
DR PRIDE; Q22830; -.
DR EnsemblMetazoa; T27B1.1.1; T27B1.1.1; WBGene00003883.
DR GeneID; 181715; -.
DR KEGG; cel:CELE_T27B1.1; -.
DR UCSC; T27B1.1; c. elegans.
DR CTD; 181715; -.
DR WormBase; T27B1.1; CE41845; WBGene00003883; osm-1.
DR eggNOG; KOG3616; Eukaryota.
DR GeneTree; ENSGT00940000153417; -.
DR HOGENOM; CLU_002716_0_0_1; -.
DR InParanoid; Q22830; -.
DR OMA; YQQLGMW; -.
DR OrthoDB; 30851at2759; -.
DR PhylomeDB; Q22830; -.
DR Reactome; R-CEL-5620924; Intraflagellar transport.
DR PRO; PR:Q22830; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00003883; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005930; C:axoneme; IBA:GO_Central.
DR GO; GO:0036064; C:ciliary basal body; IBA:GO_Central.
DR GO; GO:0035869; C:ciliary transition zone; IDA:WormBase.
DR GO; GO:0005929; C:cilium; ISS:UniProtKB.
DR GO; GO:0030992; C:intraciliary transport particle B; IDA:WormBase.
DR GO; GO:0097730; C:non-motile cilium; IDA:WormBase.
DR GO; GO:0060271; P:cilium assembly; IGI:UniProtKB.
DR GO; GO:0043053; P:dauer entry; IGI:UniProtKB.
DR GO; GO:0042073; P:intraciliary transport; IBA:GO_Central.
DR GO; GO:1905515; P:non-motile cilium assembly; IMP:WormBase.
DR Gene3D; 1.25.40.10; -; 1.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Developmental protein; Reference proteome; Repeat;
KW TPR repeat; WD repeat.
FT CHAIN 1..1737
FT /note="Intraflagellar transport protein osm-1"
FT /id="PRO_0000328945"
FT REPEAT 14..53
FT /note="WD 1"
FT REPEAT 63..103
FT /note="WD 2"
FT REPEAT 110..150
FT /note="WD 3"
FT REPEAT 151..189
FT /note="WD 4"
FT REPEAT 191..229
FT /note="WD 5"
FT REPEAT 233..273
FT /note="WD 6"
FT REPEAT 511..553
FT /note="WD 7"
FT REPEAT 700..737
FT /note="TPR 1"
FT REPEAT 803..836
FT /note="TPR 2"
FT REPEAT 848..881
FT /note="TPR 3"
FT REPEAT 907..940
FT /note="TPR 4"
FT REPEAT 979..1012
FT /note="TPR 5"
FT REPEAT 1037..1070
FT /note="TPR 6"
FT REPEAT 1137..1170
FT /note="TPR 7"
SQ SEQUENCE 1737 AA; 195702 MW; EE96E0B3D871ADAA CRC64;
MKLKYLSTIL PAQDGEAKIS NISCSPNGSR AAIACSDRSV ALLDENGVQK DRFTCKPIDA
KYGKKSFTVL CMTFSPDSSR IAIGQSDNVL FIYKVGTSWN EKKVIVNKFV QPSAVTCLSW
PFDDKILVGQ LDGKVRIGLI KTNKCSSLYK TDETVVSIQT HPKRTSFVSA HQDGSIILYN
FSSRTQSKIC TLQVPPYNLV FTNHGLVVAT SDRRVLSYTE NGVVQQQFDY NDQSEKEFSS
ISCDPTAQNV VVASYDRLRL FSWSARRGAW DEGAPLEIQN AYTIGALGWK MDGSTIYAGT
VCGGVFSVDC CLRRGMLKSR FETTYVAPSH VILRDVTNDT RTNVISNKGL AIDELKIMGK
DRYVIGYTSS SIIIADTESQ RFSELEWQSG GHEKFYFDFN NCCLIINAGE VTVVEYGVDG
SLGWVRTELT SPHLLSVQVS GPDVEEHKKV KKLAYLVDPT TISIINLING QQESFINHTG
AVDWIELNER ASKLLYRDKR SKVTLVDISS DQRSVLLSFC TYVQWVPMSD VIVAQSGDNL
SIWYNPDLPE QVTNMKIKGE VEAVLRDADR TEVIVQEPTA KVAYELDNTQ IEFGAALEKR
DFDRAVAFLE SNTSGTDAYS MWIRVAEMAL EHGNLFVAQR CYAAINDVAK VRKLHDILEI
ADEASISIGG DGTHFYKVRA MLAIMGRKFK EAERIFLEQN DTESAIGMYT SLHKWDEALE
LAKVLNYPEY EQLKTSYLRA LSDTGQDSKA AELKVSDGDT LSAIQLYIKS NKPLSALSAA
NNDSVLSQDE NILRQIADSL VKSQLYDKAG DVYEKLKDFD KAVEYFKKGD AYGKAIQLAR
FAFPEKVVTL EQEWGLHLEY IGQYDAAVNH FVEANDLKKA VEAAIRAKEW PKALSIVENI
QDQKVRTGYY GEIADHYSNK GDFERAERLF VEAGLFNDAI MMYGKNNKWI DAFRLSEEFH
GREATISSYL AKAEDLDEHG RFAEAEQLYI TIGMPHKAIQ MYDRVGRDDD VLRLVERYHG
EHMHETRKRF ATQYEERGDL KAAEEQFLKA GDFRSAVNMY KDSEMWSDAY RIAKTEGGEN
MEKQVLFMWA KSIGGDAAVK LLNKHGMLME GIDFACETGA FDLAFDLARI GAKDRMGTVH
VRLATQLEEE GRLEDASKHY VEGNKPELAV EMFIRDNDWA DAERVAKDHC ESLLPDVYTG
QARRAIEEGD HLRAETFLLR ANKPDIILRY FIENEMWPDA LRIAQNYLPH QAALIQEEYE
KSELRNGARG VDSFVAQAKE WEQQGDWRKA VSALLKINRD STDNDALIKH STEKAADLVM
KFLMGDEEYI GAALGALDEA NCNEKAAELL LLFGQSRQAI NALCRAKQWA KAKQVAQEYL
PEMVPEIEKI YKESLKSEGR LGELIDVDVI TAIDMMIEND QWDKALDTAK SQNYRPLLDK
YVAQYAAILV HRNDLSRVLA VLERYGASAN PANFSIYKLL MEETLAKPRF DYTEIARVRN
VHLDVYNALQ KESSEHFEEF SRALWALHLI AMRTALEEIG DSVPEVQKLC LKQSLSLLRY
TDILVADRIF YEAGAAAKDY GSEYESLGFL LLNHYLDLVD AIEEGNGELV DYSPFENSDI
PTEVSLPTRQ WLESAKHEEM KEWVLASSVD DAHAKELVYD KRGVFEASLK DKRGTAEPCL
VTGYPVIEST VRIGSMVAEK DNLNKFLVVI KSNQTENLLN VQNFVAKWAG SPLAISL
