ID   OSMO_CALPC              Reviewed;          40 AA.
AC   P86363;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Osmotin {ECO:0000303|PubMed:21334906};
DE            Short=CpOsm {ECO:0000303|PubMed:21334906};
DE   Flags: Fragment;
OS   Calotropis procera (Roostertree) (Asclepias procera).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Asclepiadoideae; Asclepiadeae;
OC   Asclepiadinae; Calotropis.
OX   NCBI_TaxID=141467;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Latex {ECO:0000269|PubMed:21334906};
RX   PubMed=21334906; DOI=10.1016/j.plaphy.2011.01.027;
RA   Teixeira de Freitas C.D., Sousa Nogueira F.C., Vasconcelos I.M.,
RA   Abreu Oliveira J.T., Domont G.B., Ramos M.V.;
RT   "Osmotin purified from the latex of Calotropis procera: Biochemical
RT   characterization, biological activity and role in plant defense.";
RL   Plant Physiol. Biochem. 49:738-743(2011).
CC   -!- FUNCTION: Has antifungal activity. Inhibits spore germination and
CC       mycelia growth in F.solani (IC(50)=67.0 ug/ml), C.gloeosporioides
CC       (IC(50)=32.1 ug/ml) and a Neurospora isolate (IC(50)=57.5 ug/ml).
CC       {ECO:0000269|PubMed:21334906}.
CC   -!- MASS SPECTROMETRY: Mass=22340; Method=MALDI; Note=Isoform 1.;
CC       Evidence={ECO:0000269|PubMed:21334906};
CC   -!- MASS SPECTROMETRY: Mass=22536; Method=MALDI; Note=Isoform 2.;
CC       Evidence={ECO:0000269|PubMed:21334906};
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of isoform 1 is: 8.9,
CC       its MW is: 22 kDa. {ECO:0000269|PubMed:21334906}.
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of isoform 2 is: 9.1,
CC       its MW is: 22 kDa. {ECO:0000269|PubMed:21334906}.
CC   -!- SIMILARITY: Belongs to the thaumatin family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00699}.
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DR   AlphaFoldDB; P86363; -.
DR   SMR; P86363; -.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.110.10; -; 1.
DR   InterPro; IPR037176; Osmotin/thaumatin-like_sf.
DR   InterPro; IPR001938; Thaumatin.
DR   PANTHER; PTHR31048; PTHR31048; 1.
DR   PRINTS; PR00347; THAUMATIN.
DR   SUPFAM; SSF49870; SSF49870; 1.
DR   PROSITE; PS51367; THAUMATIN_2; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW   Pathogenesis-related protein; Plant defense.
FT   CHAIN           1..>40
FT                   /note="Osmotin"
FT                   /id="PRO_0000408760"
FT   DISULFID        9..?
FT                   /evidence="ECO:0000250|UniProtKB:P14170,
FT                   ECO:0000255|PROSITE-ProRule:PRU00699"
FT   NON_TER         40
FT                   /evidence="ECO:0000303|PubMed:21334906"
SQ   SEQUENCE   40 AA;  4134 MW;  85B2CE53ED8FD027 CRC64;
     ATFTIRNNCP YTIWAAAVPG GGRRLNSGGT WTINVAPGTA