OSMO_TOBAC
ID OSMO_TOBAC Reviewed; 246 AA.
AC P14170; Q40529; Q6LDD7;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Osmotin;
DE Flags: Precursor;
GN Name=AP24;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Samsun NN; TISSUE=Leaf;
RX PubMed=8448358; DOI=10.1007/bf00014542;
RA Melchers L.S., Sela-Buurlage M.B., Vloemans S.A., Woloshuk C.P.,
RA van Roekel J.S.C., Pen J., van den Elzen P.J.M., Cornelissen B.J.C.;
RT "Extracellular targeting of the vacuolar tobacco proteins AP24, chitinase
RT and beta-1,3-glucanase in transgenic plants.";
RL Plant Mol. Biol. 21:583-593(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=1385735; DOI=10.1007/bf00026784;
RA Nelson D.E., Raghothama K.G., Singh N.K., Hasegawa P.M., Bressan R.A.;
RT "Analysis of structure and transcriptional activation of an osmotin gene.";
RL Plant Mol. Biol. 19:577-588(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Wisconsin 38;
RX PubMed=16666857; DOI=10.1104/pp.90.3.1096;
RA Singh N.K., Nelson D.E., Kuhn D., Hasegawa P.M., Bressan R.A.;
RT "Molecular cloning of osmotin and regulation of its expression by ABA and
RT adaptation to low water potential.";
RL Plant Physiol. 90:1096-1101(1989).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. White Burley;
RX PubMed=1536937; DOI=10.1007/bf00040683;
RA Kumar V., Spencer M.E.;
RT "Nucleotide sequence of an osmotin cDNA from the Nicotiana tabacum cv.
RT white burley generated by the polymerase chain reaction.";
RL Plant Mol. Biol. 18:621-622(1992).
RN [5]
RP NUCLEOTIDE SEQUENCE.
RA Barnard W.M., Neale A.D.;
RT "Comparison of the 5 regulatory regions of homeologous osmotin genes from
RT Nicotiana tabacum.";
RL Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE OF 3-246, INDUCTION, AND TISSUE SPECIFICITY.
RX PubMed=2152343; DOI=10.2307/3869130;
RA Neale A.D., Wahleithner J.A., Lund M., Bonnett H.T., Kelly A.,
RA Meeks-Wagner D.R., Peacock W.J., Dennis E.S.;
RT "Chitinase, beta-1,3-glucanase, osmotin, and extensin are expressed in
RT tobacco explants during flower formation.";
RL Plant Cell 2:673-684(1990).
RN [7]
RP PROTEIN SEQUENCE OF 22-61.
RC STRAIN=cv. Samsun NN;
RX PubMed=1841721; DOI=10.2307/3869190;
RA Woloshuk C.P., Meulenhoff J.S., Sela-Buurlage M., van den Elzen P.J.,
RA Cornelissen B.J.;
RT "Pathogen-induced proteins with inhibitory activity toward Phytophthora
RT infestans.";
RL Plant Cell 3:619-628(1991).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 22-226.
RX PubMed=14705035; DOI=10.1002/prot.10571;
RA Min K., Ha S.C., Hasegawa P.M., Bressan R.A., Yun D.J., Kim K.K.;
RT "Crystal structure of osmotin, a plant antifungal protein.";
RL Proteins 54:170-173(2004).
CC -!- SUBCELLULAR LOCATION: Vacuole. Note=Vacuolar inclusion body.
CC -!- TISSUE SPECIFICITY: Highly expressed in roots and flower buds.
CC {ECO:0000269|PubMed:2152343}.
CC -!- INDUCTION: By salt stress, abscisic acid (ABA), viral infection and
CC wounding. {ECO:0000269|PubMed:2152343}.
CC -!- MISCELLANEOUS: Inhibits the germination and growth of the fungus
CC Phytophthora infestans.
CC -!- SIMILARITY: Belongs to the thaumatin family. {ECO:0000255|PROSITE-
CC ProRule:PRU00699}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA46623.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X65700; CAA46622.1; -; mRNA.
DR EMBL; X65701; CAA46623.1; ALT_INIT; Genomic_DNA.
DR EMBL; S40046; AAB22459.2; -; Genomic_DNA.
DR EMBL; M29279; AAA34089.1; -; mRNA.
DR EMBL; X61679; CAA43854.1; -; mRNA.
DR EMBL; X95308; CAA64620.1; -; Genomic_DNA.
DR EMBL; S44889; AAB23375.1; -; mRNA.
DR PIR; S30157; S30157.
DR PIR; S34794; S34794.
DR RefSeq; NP_001312374.1; NM_001325445.1.
DR RefSeq; XP_016464912.1; XM_016609426.1.
DR PDB; 1PCV; X-ray; 2.30 A; A/B=22-226.
DR PDBsum; 1PCV; -.
DR AlphaFoldDB; P14170; -.
DR SMR; P14170; -.
DR Allergome; 9618; Nic t Osmotin.
DR GeneID; 107787819; -.
DR KEGG; nta:107787819; -.
DR OrthoDB; 1135904at2759; -.
DR EvolutionaryTrace; P14170; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0006952; P:defense response; IBA:GO_Central.
DR GO; GO:0009607; P:response to biotic stimulus; IEA:UniProtKB-KW.
DR Gene3D; 2.60.110.10; -; 1.
DR InterPro; IPR037176; Osmotin/thaumatin-like_sf.
DR InterPro; IPR001938; Thaumatin.
DR InterPro; IPR017949; Thaumatin_CS.
DR PANTHER; PTHR31048; PTHR31048; 1.
DR Pfam; PF00314; Thaumatin; 1.
DR PIRSF; PIRSF002703; Thaumatin; 1.
DR PRINTS; PR00347; THAUMATIN.
DR SMART; SM00205; THN; 1.
DR SUPFAM; SSF49870; SSF49870; 1.
DR PROSITE; PS00316; THAUMATIN_1; 1.
DR PROSITE; PS51367; THAUMATIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Disulfide bond;
KW Pathogenesis-related protein; Plant defense; Reference proteome; Signal;
KW Stress response; Vacuole.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:1841721"
FT CHAIN 22..246
FT /note="Osmotin"
FT /id="PRO_0000034043"
FT DISULFID 30..225
FT DISULFID 72..82
FT DISULFID 87..93
FT DISULFID 141..213
FT DISULFID 146..196
FT DISULFID 154..164
FT DISULFID 168..177
FT DISULFID 178..183
FT CONFLICT 2..3
FT /note="GN -> EY (in Ref. 5; CAA64620)"
FT /evidence="ECO:0000305"
FT CONFLICT 15
FT /note="L -> F (in Ref. 5; CAA64620)"
FT /evidence="ECO:0000305"
FT CONFLICT 18
FT /note="Y -> C (in Ref. 5; CAA64620)"
FT /evidence="ECO:0000305"
FT CONFLICT 62
FT /note="K -> N (in Ref. 4; CAA43854)"
FT /evidence="ECO:0000305"
FT CONFLICT 66
FT /note="V -> I (in Ref. 5; CAA64620)"
FT /evidence="ECO:0000305"
FT CONFLICT 122
FT /note="V -> L (in Ref. 3; AAA34089)"
FT /evidence="ECO:0000305"
FT CONFLICT 129
FT /note="M -> I (in Ref. 3; AAA34089)"
FT /evidence="ECO:0000305"
FT CONFLICT 132
FT /note="Missing (in Ref. 3; AAA34089)"
FT /evidence="ECO:0000305"
FT CONFLICT 144..145
FT /note="IH -> L (in Ref. 3; AAA34089)"
FT /evidence="ECO:0000305"
FT CONFLICT 149
FT /note="Missing (in Ref. 3; AAA34089)"
FT /evidence="ECO:0000305"
FT CONFLICT 151..155
FT /note="NGECP -> RRMS (in Ref. 4; CAA43854)"
FT /evidence="ECO:0000305"
FT CONFLICT 156
FT /note="R -> A (in Ref. 3; AAA34089)"
FT /evidence="ECO:0000305"
FT CONFLICT 181
FT /note="G -> R (in Ref. 3; AAA34089)"
FT /evidence="ECO:0000305"
FT CONFLICT 221
FT /note="R -> K (in Ref. 5; CAA64620)"
FT /evidence="ECO:0000305"
FT STRAND 23..28
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 34..39
FT /evidence="ECO:0007829|PDB:1PCV"
FT TURN 40..42
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 43..47
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 52..56
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 63..74
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 78..85
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 88..92
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 103..108
FT /evidence="ECO:0007829|PDB:1PCV"
FT TURN 110..112
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 113..120
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 125..127
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 129..135
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 144..146
FT /evidence="ECO:0007829|PDB:1PCV"
FT HELIX 150..153
FT /evidence="ECO:0007829|PDB:1PCV"
FT TURN 156..158
FT /evidence="ECO:0007829|PDB:1PCV"
FT HELIX 167..171
FT /evidence="ECO:0007829|PDB:1PCV"
FT HELIX 174..177
FT /evidence="ECO:0007829|PDB:1PCV"
FT HELIX 187..195
FT /evidence="ECO:0007829|PDB:1PCV"
FT HELIX 206..209
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 211..214
FT /evidence="ECO:0007829|PDB:1PCV"
FT STRAND 220..224
FT /evidence="ECO:0007829|PDB:1PCV"
SQ SEQUENCE 246 AA; 26681 MW; 3CDBA991ACA2B0B1 CRC64;
MGNLRSSFVF FLLALVTYTY AATIEVRNNC PYTVWAASTP IGGGRRLDRG QTWVINAPRG
TKMARVWGRT NCNFNAAGRG TCQTGDCGGV LQCTGWGKPP NTLAEYALDQ FSGLDFWDIS
LVDGFNIPMT FAPTNPSGGK CHAIHCTANI NGECPRELRV PGGCNNPCTT FGGQQYCCTQ
GPCGPTFFSK FFKQRCPDAY SYPQDDPTST FTCPGGSTNY RVIFCPNGQA HPNFPLEMPG
SDEVAK
