ID   OSPA3_BORBG             Reviewed;         274 AA.
AC   Q09086;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Outer surface protein A;
DE   Flags: Precursor;
GN   Name=ospA;
OS   Borreliella burgdorferi (Lyme disease spirochete) (Borrelia burgdorferi).
OG   Plasmid lp54.
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX   NCBI_TaxID=139;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B29;
RX   PubMed=1398118; DOI=10.1016/0378-1119(92)90021-g;
RA   Fellinger W., Redl B., Stoeffler G.;
RT   "Sequence of the complete osp operon encoding two major outer membrane
RT   proteins of a European Borrelia burgdorferi isolate (B29).";
RL   Gene 120:127-128(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B927;
RX   PubMed=1356932; DOI=10.1128/iai.60.11.4856-4866.1992;
RA   Wallich R., Helmes C., Schaible U.E., Lobet Y., Moter S.E., Kramer M.D.,
RA   Simon M.M.;
RT   "Evaluation of genetic divergence among Borrelia burgdorferi isolates by
RT   use of OspA, fla, HSP60, and HSP70 gene probes.";
RL   Infect. Immun. 60:4856-4866(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=GOE 2;
RX   PubMed=1563775; DOI=10.1128/iai.60.5.1864-1868.1992;
RA   Eiffert H., Ohlenbusch A., Fehling W., Lotter H., Thomssen R.;
RT   "Nucleotide sequence of the ospAB operon of a Borrelia burgdorferi strain
RT   expressing OspA but not OspB.";
RL   Infect. Immun. 60:1864-1868(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=GOE 2;
RA   Rosa P.A., Hogan D., Margolis N.;
RT   "Molecular analysis of the major outer surface protein locus from a
RT   divergent Borrelia burgdorferi isolate from Europe.";
RL   Curr. Commun. Cell Mol. Biol. 6:95-110(1992).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=G25;
RX   PubMed=7626787; DOI=10.3109/10425179509030975;
RA   Godfroid E., Ben-Messaoud A., Poliszczak A., Lobet Y., Bollen A.;
RT   "Assignment of Borrelia burgdorferi strains G25 and VS461 to the Borrelia
RT   garinii and Borrelia afzelii genospecies, respectively: a comparison of
RT   OspA protein sequences.";
RL   DNA Seq. 5:251-254(1995).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PWudll, and TN;
RX   PubMed=7500914; DOI=10.1007/bf00221390;
RA   Will G., Jauris-Heipke S., Schwab E., Busch U., Roessler D., Soutschek E.,
RA   Wilske B., Preac-Mursic V.;
RT   "Sequence analysis of ospA genes shows homogeneity within Borrelia
RT   burgdorferi sensu stricto and Borrelia afzelii strains but reveals major
RT   subgroups within the Borrelia garinii species.";
RL   Med. Microbiol. Immunol. 184:73-80(1995).
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane; Lipid-anchor.
CC   -!- MISCELLANEOUS: The sequence shown is that of strain B29.
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DR   EMBL; M88764; AAA18508.1; -; Unassigned_DNA.
DR   EMBL; X60300; CAA42842.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; L19702; AAA88846.1; -; Genomic_DNA.
DR   EMBL; Z29086; CAA82328.1; -; Genomic_DNA.
DR   EMBL; X80252; CAA56545.1; -; Genomic_DNA.
DR   EMBL; X80253; CAA56546.1; -; Genomic_DNA.
DR   PIR; E49209; E49209.
DR   AlphaFoldDB; Q09086; -.
DR   SMR; Q09086; -.
DR   PRIDE; Q09086; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR001809; OM_lipoprot_Borrelia.
DR   InterPro; IPR023322; OM_lipoprot_dom_sf.
DR   Pfam; PF00820; Lipoprotein_1; 1.
DR   PRINTS; PR00968; OUTRSURFACE.
DR   SUPFAM; SSF51087; SSF51087; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Direct protein sequencing; Lipoprotein; Membrane;
KW   Palmitate; Plasmid; Signal.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           17..274
FT                   /note="Outer surface protein A"
FT                   /id="PRO_0000018076"
FT   LIPID           17
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000305"
FT   LIPID           17
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000305"
FT   VARIANT         55
FT                   /note="D -> E (in strain: GOE 2, G25, PWUDLL and TN)"
FT   VARIANT         216
FT                   /note="N -> K (in strain: GOE 2, G25, PWUDLL and TN)"
FT   VARIANT         221
FT                   /note="S -> T (in strain: GOE 2, G25, PWUDLL and TN)"
FT   VARIANT         271
FT                   /note="A -> D (in strain: GOE 2, G25, PWUDLL and TN)"
SQ   SEQUENCE   274 AA;  29591 MW;  F75A5B6570C6BBE8 CRC64;
     MKKYLLGIGL ILALIACKQN VSSLDEKNSV SVDLPGGMTV LVSKEKDKDG KYSLDATVDK
     LELKGTSDKN NGSGTLEGEK TDKSKVKLTI ADDLSQTKFE IFKEDGKTLV SKKVTLKDKS
     STEEKFNEKG ETSEKTIVRA NGTRLEYTDI KSDGSGKAKE VLKDFTLEGT LAADGKTTLK
     VTEGTVVLSK NILKSGEITV ALDDSDTTQA TKKTGNWDSK SSTLTISVNS QKTKNLVFTK
     EDTITVQKYD SAGTNLEGKA VEITTLKELK AALK