ASB8_HUMAN
ID ASB8_HUMAN Reviewed; 288 AA.
AC Q9H765; A8K1P2; Q547Q2;
DT 28-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Ankyrin repeat and SOCS box protein 8;
DE Short=ASB-8;
GN Name=ASB8; ORFNames=PP14212;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=12559969; DOI=10.1016/s0006-291x(02)02971-6;
RA Liu Y., Li J., Zhang F., Qin W., Yao G., He X., Xue P., Ge C., Wan D.,
RA Gu J.;
RT "Molecular cloning and characterization of the human ASB-8 gene encoding a
RT novel member of ankyrin repeat and SOCS box containing protein family.";
RL Biochem. Biophys. Res. Commun. 300:972-979(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon, and Hippocampus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Cervix;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be a substrate-recognition component of a SCF-like ECS
CC (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex
CC which mediates the ubiquitination and subsequent proteasomal
CC degradation of target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- INTERACTION:
CC Q9H765; P40337-2: VHL; NbExp=3; IntAct=EBI-3942509, EBI-12157263;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12559969}.
CC -!- TISSUE SPECIFICITY: Highest level of expression in skeletal muscle.
CC Also expressed in heart, brain, placenta, liver, kidney and pancreas.
CC {ECO:0000269|PubMed:12559969}.
CC -!- DOMAIN: The SOCS box domain mediates the interaction with the Elongin
CC BC complex, an adapter module in different E3 ubiquitin-protein ligase
CC complexes. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ankyrin SOCS box (ASB) family.
CC {ECO:0000305}.
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DR EMBL; AF464877; AAQ04830.1; -; mRNA.
DR EMBL; AK024908; BAB15033.1; -; mRNA.
DR EMBL; AK289957; BAF82646.1; -; mRNA.
DR EMBL; BC001321; AAH01321.1; -; mRNA.
DR CCDS; CCDS8761.1; -.
DR RefSeq; NP_001306225.1; NM_001319296.1.
DR RefSeq; NP_001306226.1; NM_001319297.1.
DR RefSeq; NP_001306227.1; NM_001319298.1.
DR RefSeq; NP_001306228.1; NM_001319299.1.
DR RefSeq; NP_001306229.1; NM_001319300.1.
DR RefSeq; NP_001306230.1; NM_001319301.1.
DR RefSeq; NP_077000.1; NM_024095.4.
DR AlphaFoldDB; Q9H765; -.
DR SMR; Q9H765; -.
DR BioGRID; 126614; 34.
DR IntAct; Q9H765; 16.
DR MINT; Q9H765; -.
DR STRING; 9606.ENSP00000320893; -.
DR iPTMnet; Q9H765; -.
DR PhosphoSitePlus; Q9H765; -.
DR BioMuta; ASB8; -.
DR DMDM; 29839757; -.
DR EPD; Q9H765; -.
DR jPOST; Q9H765; -.
DR MassIVE; Q9H765; -.
DR PaxDb; Q9H765; -.
DR PeptideAtlas; Q9H765; -.
DR PRIDE; Q9H765; -.
DR ProteomicsDB; 81083; -.
DR Antibodypedia; 1146; 205 antibodies from 25 providers.
DR DNASU; 140461; -.
DR Ensembl; ENST00000317697.8; ENSP00000320893.3; ENSG00000177981.11.
DR Ensembl; ENST00000536549.5; ENSP00000445622.1; ENSG00000177981.11.
DR GeneID; 140461; -.
DR KEGG; hsa:140461; -.
DR MANE-Select; ENST00000317697.8; ENSP00000320893.3; NM_024095.5; NP_077000.1.
DR UCSC; uc001rrh.4; human.
DR CTD; 140461; -.
DR DisGeNET; 140461; -.
DR GeneCards; ASB8; -.
DR HGNC; HGNC:17183; ASB8.
DR HPA; ENSG00000177981; Tissue enhanced (skeletal muscle, tongue).
DR MIM; 615053; gene.
DR neXtProt; NX_Q9H765; -.
DR OpenTargets; ENSG00000177981; -.
DR PharmGKB; PA25036; -.
DR VEuPathDB; HostDB:ENSG00000177981; -.
DR eggNOG; KOG0504; Eukaryota.
DR GeneTree; ENSGT00940000159366; -.
DR HOGENOM; CLU_000134_52_0_1; -.
DR InParanoid; Q9H765; -.
DR OMA; DDTPLSW; -.
DR OrthoDB; 896829at2759; -.
DR PhylomeDB; Q9H765; -.
DR TreeFam; TF332452; -.
DR PathwayCommons; Q9H765; -.
DR Reactome; R-HSA-8951664; Neddylation.
DR Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; Q9H765; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 140461; 8 hits in 1118 CRISPR screens.
DR ChiTaRS; ASB8; human.
DR GenomeRNAi; 140461; -.
DR Pharos; Q9H765; Tdark.
DR PRO; PR:Q9H765; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q9H765; protein.
DR Bgee; ENSG00000177981; Expressed in gastrocnemius and 204 other tissues.
DR ExpressionAtlas; Q9H765; baseline and differential.
DR Genevisible; Q9H765; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd03727; SOCS_ASB8; 1.
DR Gene3D; 1.25.40.20; -; 2.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR037332; ASB8_SOCS.
DR InterPro; IPR001496; SOCS_box.
DR InterPro; IPR036036; SOCS_box-like_dom_sf.
DR Pfam; PF12796; Ank_2; 2.
DR Pfam; PF07525; SOCS_box; 1.
DR SMART; SM00248; ANK; 4.
DR SMART; SM00969; SOCS_box; 1.
DR SUPFAM; SSF158235; SSF158235; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 4.
DR PROSITE; PS50225; SOCS; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Cytoplasm; Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..288
FT /note="Ankyrin repeat and SOCS box protein 8"
FT /id="PRO_0000066938"
FT REPEAT 52..81
FT /note="ANK 1"
FT REPEAT 85..113
FT /note="ANK 2"
FT REPEAT 117..146
FT /note="ANK 3"
FT REPEAT 150..179
FT /note="ANK 4"
FT DOMAIN 235..288
FT /note="SOCS box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00194"
SQ SEQUENCE 288 AA; 31642 MW; B10FB7ADBDC3D3D6 CRC64;
MSSSMWYIMQ SIQSKYSLSE RLIRTIAAIR SFPHDNVEDL IRGGADVNCT HGTLKPLHCA
CMVSDADCVE LLLEKGAEVN ALDGYNRTAL HYAAEKDEAC VEVLLEYGAN PNALDGNRDT
PLHWAAFKNN AECVRALLES GASVNALDYN NDTPLSWAAM KGNLESVSIL LDYGAEVRVI
NLIGQTPISR LVALLVRGLG TEKEDSCFEL LHRAVGHFEL RKNGTMPREV ARDPQLCEKL
TVLCSAPGTL KTLARYAVRR SLGLQYLPDA VKGLPLPASL KEYLLLLE
