OSPF_SHIBO
ID OSPF_SHIBO Reviewed; 170 AA.
AC Q2ET28;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 30.
DE RecName: Full=Phosphothreonine lyase OspF;
DE EC=4.2.3.-;
DE AltName: Full=Effector protein OspF;
DE Flags: Fragment;
GN Name=ospF;
OS Shigella boydii.
OG Plasmid pSb17_G1214, Plasmid pSb2_G1184, Plasmid pSb3_G1232,
OG Plasmid pSb6_G1227, Plasmid pSb7_G1187, Plasmid pSb8_G1268,
OG Plasmid pSb9_G1236, Plasmid pSb1_G1228, Plasmid pSb10_G1294,
OG Plasmid pSb11_G1191, Plasmid pSb12_G1287, Plasmid pSb14_G1300, and
OG Plasmid pSb15_G1282.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=621;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=G1184 / Serotype 2, G1187 / Serotype 7, G1191 / Serotype 11,
RC G1214 / Serotype 17, G1227 / Serotype 6, G1228 / Serotype 1,
RC G1232 / Serotype 3, G1236 / Serotype 9, G1268 / Serotype 8,
RC G1282 / Serotype 15, G1287 / Serotype 12, G1294 / Serotype 10, and
RC G1300 / Serotype 14;
RC PLASMID=pSb10_G1294, pSb11_G1191, pSb12_G1287, pSb14_G1300, pSb15_G1282,
RC pSb17_G1214, pSb1_G1228, pSb2_G1184, pSb3_G1232, pSb6_G1227, pSb7_G1187,
RC pSb8_G1268, and pSb9_G1236;
RX PubMed=17160643; DOI=10.1007/s00239-006-0052-8;
RA Yang J., Nie H., Chen L., Zhang X., Yang F., Xu X., Zhu Y., Yu J., Jin Q.;
RT "Revisiting the molecular evolutionary history of Shigella spp.";
RL J. Mol. Evol. 64:71-79(2007).
CC -!- FUNCTION: Catalyzes the removal of the phosphate group from the
CC phosphothreonine in the mitogen-activated protein kinases such as
CC MAPK2/ERK2, MAPK3/ERK1, MAPK8 and MAPK14 in an irreversible reaction,
CC thus preventing the downstream phosphorylation of histone H3. This
CC epigenetic modification results in inhibition of the transcription of a
CC specific subset of pro-inflammatory genes, and ultimately to a reduced
CC immune response against the invading pathogen. The diminished immune
CC response enhances the bacterium's ability to disseminate and multiply
CC within the host (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted. Note=Secreted via the type III
CC secretion system (TTSS). Localizes in the nucleus of the infected cell
CC (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the phosphothreonine lyase family.
CC {ECO:0000305}.
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DR EMBL; DQ362851; ABD37258.1; -; Genomic_DNA.
DR EMBL; DQ362852; ABD37259.1; -; Genomic_DNA.
DR EMBL; DQ362853; ABD37260.1; -; Genomic_DNA.
DR EMBL; DQ362854; ABD37261.1; -; Genomic_DNA.
DR EMBL; DQ362855; ABD37262.1; -; Genomic_DNA.
DR EMBL; DQ362856; ABD37263.1; -; Genomic_DNA.
DR EMBL; DQ362857; ABD37264.1; -; Genomic_DNA.
DR EMBL; DQ362858; ABD37265.1; -; Genomic_DNA.
DR EMBL; DQ362859; ABD37266.1; -; Genomic_DNA.
DR EMBL; DQ362860; ABD37267.1; -; Genomic_DNA.
DR EMBL; DQ362861; ABD37268.1; -; Genomic_DNA.
DR EMBL; DQ362862; ABD37269.1; -; Genomic_DNA.
DR EMBL; DQ362863; ABD37270.1; -; Genomic_DNA.
DR AlphaFoldDB; Q2ET28; -.
DR SMR; Q2ET28; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.2430.10; -; 1.
DR InterPro; IPR003519; OspF/SpvC.
DR InterPro; IPR038498; OspF/SpvC_sf.
DR Pfam; PF03536; VRP3; 1.
DR PRINTS; PR01342; SALVRPPROT.
PE 3: Inferred from homology;
KW Lyase; Plasmid; Secreted; Virulence.
FT CHAIN <1..>170
FT /note="Phosphothreonine lyase OspF"
FT /id="PRO_0000299350"
FT ACT_SITE 82
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 112
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT NON_TER 1
FT NON_TER 170
SQ SEQUENCE 170 AA; 19585 MW; B2D0850BF4172738 CRC64;
PQMLSANERL KNNFNILYNQ IRQYPAYYFK VASNVPTYSD ICQFFSVMYQ GFQIVNHSGD
VFIHACRENP QSKGDFVGDK FHISIAREQV PLAFQILSGL LFSEDSPIDK WKITDMNRVS
QQSRVGIGAQ FTLYVKSDQE CSQYSALLLH KIRQFIMCLE SNLLRSKIAP
