ID   ASB8_MOUSE              Reviewed;         288 AA.
AC   Q91ZT9; Q8R178;
DT   28-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 150.
DE   RecName: Full=Ankyrin repeat and SOCS box protein 8;
DE            Short=ASB-8;
GN   Name=Asb8;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11509662; DOI=10.1128/mcb.21.18.6189-6197.2001;
RA   Kile B.T., Metcalf D., Mifsud S., DiRago L., Nicola N.A., Hilton D.J.,
RA   Alexander W.S.;
RT   "Functional analysis of Asb-1 using genetic modification in mice.";
RL   Mol. Cell. Biol. 21:6189-6197(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: May be a substrate-recognition component of a SCF-like ECS
CC       (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex
CC       which mediates the ubiquitination and subsequent proteasomal
CC       degradation of target proteins. {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DOMAIN: The SOCS box domain mediates the interaction with the Elongin
CC       BC complex, an adapter module in different E3 ubiquitin-protein ligase
CC       complexes. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ankyrin SOCS box (ASB) family.
CC       {ECO:0000305}.
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DR   EMBL; AF398969; AAK97491.1; -; mRNA.
DR   EMBL; BC025106; AAH25106.1; -; mRNA.
DR   CCDS; CCDS27787.1; -.
DR   RefSeq; NP_001164181.1; NM_001170710.1.
DR   RefSeq; NP_001164182.1; NM_001170711.1.
DR   RefSeq; NP_084397.3; NM_030121.4.
DR   RefSeq; XP_006521605.1; XM_006521542.3.
DR   RefSeq; XP_011244077.1; XM_011245775.2.
DR   AlphaFoldDB; Q91ZT9; -.
DR   SMR; Q91ZT9; -.
DR   STRING; 10090.ENSMUSP00000115813; -.
DR   iPTMnet; Q91ZT9; -.
DR   PhosphoSitePlus; Q91ZT9; -.
DR   EPD; Q91ZT9; -.
DR   MaxQB; Q91ZT9; -.
DR   PaxDb; Q91ZT9; -.
DR   PRIDE; Q91ZT9; -.
DR   ProteomicsDB; 277253; -.
DR   DNASU; 78541; -.
DR   GeneID; 78541; -.
DR   KEGG; mmu:78541; -.
DR   UCSC; uc007xlx.2; mouse.
DR   CTD; 140461; -.
DR   MGI; MGI:1925791; Asb8.
DR   eggNOG; KOG0504; Eukaryota.
DR   InParanoid; Q91ZT9; -.
DR   OrthoDB; 896829at2759; -.
DR   TreeFam; TF332452; -.
DR   Reactome; R-MMU-8951664; Neddylation.
DR   Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00143; -.
DR   BioGRID-ORCS; 78541; 1 hit in 73 CRISPR screens.
DR   ChiTaRS; Asb8; mouse.
DR   PRO; PR:Q91ZT9; -.
DR   Proteomes; UP000000589; Unplaced.
DR   RNAct; Q91ZT9; protein.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   CDD; cd03727; SOCS_ASB8; 1.
DR   Gene3D; 1.25.40.20; -; 2.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR037332; ASB8_SOCS.
DR   InterPro; IPR001496; SOCS_box.
DR   InterPro; IPR036036; SOCS_box-like_dom_sf.
DR   Pfam; PF12796; Ank_2; 1.
DR   Pfam; PF13637; Ank_4; 1.
DR   Pfam; PF07525; SOCS_box; 1.
DR   SMART; SM00248; ANK; 4.
DR   SMART; SM00969; SOCS_box; 1.
DR   SUPFAM; SSF158235; SSF158235; 1.
DR   SUPFAM; SSF48403; SSF48403; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 4.
DR   PROSITE; PS50225; SOCS; 1.
PE   2: Evidence at transcript level;
KW   ANK repeat; Cytoplasm; Reference proteome; Repeat; Ubl conjugation pathway.
FT   CHAIN           1..288
FT                   /note="Ankyrin repeat and SOCS box protein 8"
FT                   /id="PRO_0000066939"
FT   REPEAT          52..81
FT                   /note="ANK 1"
FT   REPEAT          85..113
FT                   /note="ANK 2"
FT   REPEAT          117..146
FT                   /note="ANK 3"
FT   REPEAT          150..179
FT                   /note="ANK 4"
FT   DOMAIN          235..288
FT                   /note="SOCS box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00194"
FT   CONFLICT        96
FT                   /note="R -> K (in Ref. 2; AAH25106)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   288 AA;  31735 MW;  363727C6EB46CB1D CRC64;
     MSSSMWYIMQ SIQSKYSLSE RLIRTIAAIR SFPHDNVEDL IRGGADVNCT HGTLKPLHCA
     CMVSDADCVE LLLEKGAEVN ALDGYNRTAL HYAAERDEAC VEVLLEYGAN PNALDGNRDT
     PLHWAAFKNN AECVRALLES GASVNALDYN NDTPLSWAAM KGNLESVSIL LDYGAEVRVI
     NLKGQTPISR LVALLVRGLG TEKEDSCFEL LHRAVGQFEL RKNGIMPREV TKDQQLCEKL
     TVLCSAPGTL KTLARYAVRR SLGLQYLPDA VKGLPLPVSL KDYLLLLE