ID   OSPF_SHISS              Reviewed;         239 AA.
AC   Q3YTY3;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Phosphothreonine lyase OspF;
DE            EC=4.2.3.-;
DE   AltName: Full=Effector protein OspF;
GN   Name=ospF; Synonyms=mkaD; OrderedLocusNames=SSON_P009;
OS   Shigella sonnei (strain Ss046).
OG   Plasmid pSS_046.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=300269;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ss046;
RX   PubMed=16275786; DOI=10.1093/nar/gki954;
RA   Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J.,
RA   Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J.,
RA   Xu J., Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J.,
RA   Jin Q.;
RT   "Genome dynamics and diversity of Shigella species, the etiologic agents of
RT   bacillary dysentery.";
RL   Nucleic Acids Res. 33:6445-6458(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ss046;
RX   PubMed=16122562; DOI=10.1016/j.plasmid.2005.03.002;
RA   Jiang Y., Yang F., Zhang X., Yang J., Chen L., Yan Y., Nie H., Xiong Z.,
RA   Wang J., Dong J., Xue Y., Xu X., Zhu Y., Chen S., Jin Q.;
RT   "The complete sequence and analysis of the large virulence plasmid pSS of
RT   Shigella sonnei.";
RL   Plasmid 54:149-159(2005).
CC   -!- FUNCTION: Catalyzes the removal of the phosphate group from the
CC       phosphothreonine in the mitogen-activated protein kinases such as
CC       MAPK2/ERK2, MAPK3/ERK1, MAPK8 and MAPK14 in an irreversible reaction,
CC       thus preventing the downstream phosphorylation of histone H3. This
CC       epigenetic modification results in inhibition of the transcription of a
CC       specific subset of pro-inflammatory genes, and ultimately to a reduced
CC       immune response against the invading pathogen. The diminished immune
CC       response enhances the bacterium's ability to disseminate and multiply
CC       within the host (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted. Note=Secreted via the type III
CC       secretion system (TTSS). Localizes in the nucleus of the infected cell
CC       (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the phosphothreonine lyase family.
CC       {ECO:0000305}.
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DR   EMBL; CP000039; AAZ91029.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q3YTY3; -.
DR   SMR; Q3YTY3; -.
DR   EnsemblBacteria; AAZ91029; AAZ91029; SSON_P009.
DR   KEGG; ssn:SSON_P009; -.
DR   HOGENOM; CLU_100525_0_0_6; -.
DR   OMA; RVDQQSR; -.
DR   Proteomes; UP000002529; Plasmid pSS_046.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.2430.10; -; 1.
DR   InterPro; IPR003519; OspF/SpvC.
DR   InterPro; IPR038498; OspF/SpvC_sf.
DR   Pfam; PF03536; VRP3; 1.
DR   PRINTS; PR01342; SALVRPPROT.
PE   3: Inferred from homology;
KW   Lyase; Plasmid; Secreted; Virulence.
FT   CHAIN           1..239
FT                   /note="Phosphothreonine lyase OspF"
FT                   /id="PRO_0000299355"
FT   ACT_SITE        104
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        134
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   239 AA;  27901 MW;  95A9F09DD4207A3B CRC64;
     MPIKKPCLKL NLDSLNVVRS EIPQMLSANE RLKNNFNILY NQIRQYPAYY FKVASNVPNY
     SDICQFFSVM YQGFQIVNHS GDVFIHACRE NPQSKGDFVG DKFHISIARE QVPLAFQILS
     GLLFSEDSPI DKWKITDMNR VSQQSRVGIG AQFTLYVKSD QECSQYSALL LHKIRQFIMC
     LESNLLRSKI APGEYPASDV RPEDWKYVSY RNELRSDRDG SERQEQMLRE EPFYRLMIE