OSP_DROME
ID OSP_DROME Reviewed; 1566 AA.
AC Q27421; A2VEG2; A9UN92; B7YZW1; E1JHH8; Q24231; Q7KT89; Q8MSX4; Q9VJS4;
DT 27-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 5.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Protein outspread;
GN Name=osp; ORFNames=CG3479;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10471707; DOI=10.1093/genetics/153.1.179;
RA Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T.,
RA Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G., Harvey D.A.,
RA Hong L., Houston K.A., Hoskins R.A., Johnson G., Martin C., Moshrefi A.R.,
RA Palazzolo M., Reese M.G., Spradling A.C., Tsang G., Wan K.H., Whitelaw K.,
RA Celniker S.E., Rubin G.M.;
RT "An exploration of the sequence of a 2.9-Mb region of the genome of
RT Drosophila melanogaster: the Adh region.";
RL Genetics 153:179-219(1999).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D), NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-1374 (ISOFORM C/F), AND NUCLEOTIDE SEQUENCE [LARGE
RP SCALE MRNA] OF 465-1566 (ISOFORM E).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 130-284.
RX PubMed=6410283; DOI=10.1038/304412a0;
RA Kreitman M.;
RT "Nucleotide polymorphism at the alcohol dehydrogenase locus of Drosophila
RT melanogaster.";
RL Nature 304:412-417(1983).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=F;
CC IsoId=Q27421-4; Sequence=Displayed;
CC Name=D;
CC IsoId=Q27421-1; Sequence=VSP_041631;
CC Name=E;
CC IsoId=Q27421-2; Sequence=VSP_041631, VSP_004064, VSP_004065;
CC Name=C;
CC IsoId=Q27421-3; Sequence=VSP_004064, VSP_004065;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA70209.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAA70211.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAM49881.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA77329.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014134; AAF53402.4; -; Genomic_DNA.
DR EMBL; AE014134; AAN10878.3; -; Genomic_DNA.
DR EMBL; AE014134; ACL83032.1; -; Genomic_DNA.
DR EMBL; AE014134; ACZ94261.1; -; Genomic_DNA.
DR EMBL; AY118512; AAM49881.1; ALT_INIT; mRNA.
DR EMBL; BT030131; ABN49270.1; -; mRNA.
DR EMBL; BT031256; ABY20497.1; -; mRNA.
DR EMBL; M17837; AAA70211.1; ALT_INIT; Genomic_DNA.
DR EMBL; M19547; AAA70209.1; ALT_INIT; Genomic_DNA.
DR EMBL; Z00030; CAA77329.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_001137826.1; NM_001144354.3. [Q27421-3]
DR RefSeq; NP_001162975.1; NM_001169504.2. [Q27421-4]
DR RefSeq; NP_523567.5; NM_078843.6. [Q27421-2]
DR RefSeq; NP_723879.3; NM_165089.4. [Q27421-1]
DR AlphaFoldDB; Q27421; -.
DR SMR; Q27421; -.
DR BioGRID; 60874; 2.
DR IntAct; Q27421; 11.
DR STRING; 7227.FBpp0290711; -.
DR PaxDb; Q27421; -.
DR PRIDE; Q27421; -.
DR DNASU; 34850; -.
DR EnsemblMetazoa; FBtr0114465; FBpp0112957; FBgn0003016. [Q27421-3]
DR EnsemblMetazoa; FBtr0301494; FBpp0290709; FBgn0003016. [Q27421-1]
DR EnsemblMetazoa; FBtr0301495; FBpp0290710; FBgn0003016. [Q27421-2]
DR EnsemblMetazoa; FBtr0301496; FBpp0290711; FBgn0003016. [Q27421-4]
DR GeneID; 34850; -.
DR KEGG; dme:Dmel_CG3479; -.
DR CTD; 34850; -.
DR FlyBase; FBgn0003016; osp.
DR VEuPathDB; VectorBase:FBgn0003016; -.
DR eggNOG; KOG4807; Eukaryota.
DR GeneTree; ENSGT00940000168923; -.
DR InParanoid; Q27421; -.
DR OMA; FSHHSQY; -.
DR PhylomeDB; Q27421; -.
DR SignaLink; Q27421; -.
DR BioGRID-ORCS; 34850; 0 hits in 3 CRISPR screens.
DR ChiTaRS; osp; fly.
DR GenomeRNAi; 34850; -.
DR PRO; PR:Q27421; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0003016; Expressed in second segment of antenna (Drosophila) and 31 other tissues.
DR Genevisible; Q27421; DM.
DR GO; GO:0015629; C:actin cytoskeleton; ISS:FlyBase.
DR GO; GO:0051015; F:actin filament binding; ISS:FlyBase.
DR GO; GO:0007015; P:actin filament organization; IBA:GO_Central.
DR CDD; cd13275; PH_M-RIP; 1.
DR Gene3D; 2.30.29.30; -; 2.
DR InterPro; IPR039597; M-RIP_PH.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR Pfam; PF00169; PH; 2.
DR SMART; SM00233; PH; 2.
DR PROSITE; PS50003; PH_DOMAIN; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Repeat.
FT CHAIN 1..1566
FT /note="Protein outspread"
FT /id="PRO_0000058089"
FT DOMAIN 84..190
FT /note="PH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 506..605
FT /note="PH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 194..318
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 373..415
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 483..509
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 627..728
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 976..1004
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1375..1438
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1514..1544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 207..269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 270..291
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 298..318
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 383..415
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 486..500
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 689..706
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 976..991
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1391..1419
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1420..1434
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1514..1539
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 72..80
FT /note="Missing (in isoform D and isoform E)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_041631"
FT VAR_SEQ 1386
FT /note="P -> V (in isoform C and isoform E)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_004064"
FT VAR_SEQ 1387..1566
FT /note="Missing (in isoform C and isoform E)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_004065"
SQ SEQUENCE 1566 AA; 175507 MW; FCB3E900080D2651 CRC64;
MSTTTITTTV PAAPSKSAPP TPTTTGAAGA TTNARTADCR KFTPNIFNKS KCSHCFRQRE
EHSAAALECN RRTSSNLAEP ASRKVSKCGY LFVAPDWDFS NPLYRTKRWQ RRWFVLYDDG
ELTYSVDDYP ETIPQACVDM TKVLEVTSAV EVTGHPNSIA ITAPERVTFV KGTSSEESQW
WLNILAAFPK SKGRHKRSAT LPGGQVVGSL RPTSNGDLTL STKLGNRHSS YHKDTLTSSQ
SAGNLLSSLD LGPSSTKTAG SPLTTTQSAL ADDEEDDGVE TGEDVDEDEE DETSVPRKSK
TVSMGGQDEN NRNAGNEITN RVSQPTTCLL IEDIRRDEKT IKDIANTITN LSQQQNKRWS
TAVNNALNNQ HHFGHHSQYQ VTSRDETDFQ MSSNSSSTKS QNPASERPKS LPLASNSTPA
IVSAIVKKIP TVMEQGDKTK PTARLQLHLK SPKHYQHERG DPDGGCNLDE LCVNYMAKTD
ELRSVGKANS KSSSGQGKPP VKEESLNAKK GWLMKQDNRT CEWSKHWFTL SGAALFYYRD
PLCEERGVLD GVLDVNSLTS VIPEPAASKQ HAFQLTTWDK QRLVLASLSP SSRNSWLAVL
RSAAGLPQLD TPPKQTDIEQ DFIKAQLQQP SSSPVTPGTP AGPHFSSDEE YRTASEGGRR
DSLDWGSPLS PSPPVLRSCL RNRSLASLHK RSRSSPPSSR RSTVDSVASD ELPLLVVPEE
MQPTESRELK QQCETLRAEA SLREARMSEL LATLQRTEQQ LTARLQEQQQ QLNSELTQAK
QSASDLMHNL GMQLTESQCQ IKQLEDRLAQ GIEENEGLYK RLRELQAQDH SGGAALSNLQ
RHKIKRMDSL SDLTTISDID PYCLQRDSLA EEYNELRSRF EKAVNEIRAM KRELKQSQNQ
YDALELAQAA LQQKLERRQH EDGAQLQLMA ARIQDLTLKY SSSERQVRAL KQKLAKSERR
RSLSLKGKEQ LELKLSELQR ETVERKEGTP PESSSSESSS QSPLNAHLLQ RLHSLEHVLL
GSKERLEQSL TQLQQIRAGQ RTRRSVSPMN DRKDGLRQLE RALAETCVMV SEQMELTCLQ
DSCHKCCDLR QRVEKLSALQ QQTETDLQRS EQLLEQRETD LAQALEKCAS QEQEQELLLQ
QRQELSEELG RQQERCRRLE KRLELLEREH GKQLECLREV YHTEHANAAD EQSFRKRYQT
EIEQLRTLCE KGLSAMETSH KRLTMDLEQK HKMEIERLLA EKETALAEET QATLAALDAM
RKAHQSEVQR EVARFKQEFL RQVQRGEQMR GDGAKLKEED LGELRMEILA FSEKYSIKCV
ENAALEEKLH MANSKLRHFQ QMQQLELRNK QFRAHLASDD PSNDVHFVQG LTSDAREDAD
CEDSEPAPQI LGATATRTTA TTTTTATATT TSAAPSETES NPDSDRETAD SSRAPPEKME
QSLFVIPSHM LNSSLVPAAN ASDQSQRSQL YRDLDGPEDG YEPCYRPFDI FAIYQNRLSY
QGLKGSSTFG KSLRKSAAQT SPASSEITTT TTAPTTDPKK PSHKQMFKTA AVINIQQQVA
QEEKAQ
