OSR1_DANRE
ID OSR1_DANRE Reviewed; 264 AA.
AC Q5XJQ7;
DT 19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Protein odd-skipped-related 1 {ECO:0000312|EMBL:AAH83241.1};
DE Short=zOsr1 {ECO:0000303|PubMed:17011543};
GN Name=osr1 {ECO:0000312|ZFIN:ZDB-GENE-070321-1};
GN ORFNames=si:dkey-199e17.1, zgc:101674;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000312|EMBL:CAX12570.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo {ECO:0000312|EMBL:AAH83241.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=17011543; DOI=10.1016/j.ydbio.2006.08.063;
RA Tena J.J., Neto A., de la Calle-Mustienes E., Bras-Pereira C., Casares F.,
RA Gomez-Skarmeta J.L.;
RT "Odd-skipped genes encode repressors that control kidney development.";
RL Dev. Biol. 301:518-531(2007).
RN [4] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=18787069; DOI=10.1242/dev.022830;
RA Mudumana S.P., Hentschel D., Liu Y., Vasilyev A., Drummond I.A.;
RT "odd skipped related1 reveals a novel role for endoderm in regulating
RT kidney versus vascular cell fate.";
RL Development 135:3355-3367(2008).
CC -!- FUNCTION: Transcriptional repressor (By similarity). Regulates mesoderm
CC versus endoderm differentiation during gastrulation to regulate kidney
CC versus vascular cell fate. Acts upstream of pax2a in kidney development
CC and functions in glomerular maturation, after podocyte specification.
CC {ECO:0000250|UniProtKB:P86413, ECO:0000269|PubMed:17011543,
CC ECO:0000269|PubMed:18787069}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P86413}.
CC -!- TISSUE SPECIFICITY: Expressed in the germ ring mesendoderm and
CC subsequently in the endoderm and intermediate mesoderm, prior to the
CC expression of definitive kidney or blood markers. At 60 hpf, expressed
CC in the glomerulus, in some patches in the eye and brain, and weakly in
CC the pectoral fin buds. {ECO:0000269|PubMed:17011543,
CC ECO:0000269|PubMed:18787069}.
CC -!- DISRUPTION PHENOTYPE: Embryos show a down-regulation of early
CC pronephric markers lhx1a/lim1 and pax2a/pax2.1, and subsequent kidney
CC defects. {ECO:0000269|PubMed:17011543, ECO:0000269|PubMed:18787069}.
CC -!- SIMILARITY: Belongs to the Odd C2H2-type zinc-finger protein family.
CC {ECO:0000255}.
CC -!- CAUTION: Contrary to PubMed:17011543, which shows pronephric expression
CC during tailbud and somitogenesis stages, PubMed:18787069 report that
CC osr1 is not expressed in kidney tissue. Similarly, in the absence of
CC osr1, PubMed:17011543 report a complete absence of kidney tissue, and
CC pericardial edemas characteristic of renal failure, whereas
CC PubMed:18787069 report a segment-specific defect in the proximal
CC nephron, with the distal nephron relatively unaffected, and a
CC compensatory increase in angioblast cell numbers in the same trunk
CC region. {ECO:0000269|PubMed:17011543, ECO:0000269|PubMed:18787069,
CC ECO:0000305}.
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DR EMBL; BX470107; CAX12570.1; -; Genomic_DNA.
DR EMBL; BC083241; AAH83241.1; -; mRNA.
DR RefSeq; NP_001006079.1; NM_001006079.1.
DR AlphaFoldDB; Q5XJQ7; -.
DR SMR; Q5XJQ7; -.
DR STRING; 7955.ENSDARP00000023564; -.
DR PaxDb; Q5XJQ7; -.
DR Ensembl; ENSDART00000020270; ENSDARP00000023564; ENSDARG00000014091.
DR Ensembl; ENSDART00000188591; ENSDARP00000146147; ENSDARG00000014091.
DR GeneID; 450059; -.
DR KEGG; dre:450059; -.
DR CTD; 130497; -.
DR ZFIN; ZDB-GENE-070321-1; osr1.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000158993; -.
DR HOGENOM; CLU_051854_0_0_1; -.
DR InParanoid; Q5XJQ7; -.
DR OMA; NAHARGP; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q5XJQ7; -.
DR TreeFam; TF350876; -.
DR PRO; PR:Q5XJQ7; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 13.
DR Bgee; ENSDARG00000014091; Expressed in ventral mesoderm and 59 other tissues.
DR ExpressionAtlas; Q5XJQ7; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0035779; P:angioblast cell differentiation; IMP:UniProtKB.
DR GO; GO:0048389; P:intermediate mesoderm development; IMP:ZFIN.
DR GO; GO:0001822; P:kidney development; IDA:ZFIN.
DR GO; GO:0048382; P:mesendoderm development; IMP:ZFIN.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0033339; P:pectoral fin development; IMP:ZFIN.
DR GO; GO:0072112; P:podocyte differentiation; IMP:ZFIN.
DR GO; GO:0035777; P:pronephric distal tubule development; IMP:UniProtKB.
DR GO; GO:0035775; P:pronephric glomerulus morphogenesis; IMP:UniProtKB.
DR GO; GO:0035778; P:pronephric nephron tubule epithelial cell differentiation; IMP:UniProtKB.
DR GO; GO:0035776; P:pronephric proximal tubule development; IMP:UniProtKB.
DR GO; GO:0048793; P:pronephros development; IDA:ZFIN.
DR GO; GO:1903224; P:regulation of endodermal cell differentiation; IMP:ZFIN.
DR GO; GO:1900107; P:regulation of nodal signaling pathway; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0001655; P:urogenital system development; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW Developmental protein; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..264
FT /note="Protein odd-skipped-related 1"
FT /id="PRO_0000390729"
FT ZN_FING 174..196
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 202..224
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 230..252
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
SQ SEQUENCE 264 AA; 29563 MW; 73C7CC8BF1A41DA4 CRC64;
MGSKTLPAPV PLHPSLQLAN YSFLQTSNGL HLPADHMPSI YSFSALHAVH LHQWTLGYPP
FTLPRCTFSK LPGLVDARFP LPSIPLFPHL VQPAKQESSG PGSGASSKSK PRFDFANLAA
AATQDDALKA EDLSTNNGHV RSPSLGCLLD VAKLSSPERK PSRGRLPSKT KKEFVCKFCG
RHFTKSYNLL IHERTHTDER PYTCDICHKA FRRQDHLRDH RYIHSKEKPF KCQECGKGFC
QSRTLAVHKT LHMQVKELKP AKIK
