OST1A_ORYSJ
ID OST1A_ORYSJ Reviewed; 615 AA.
AC Q0DJC5; A0A0P0WKA7;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A;
DE AltName: Full=Ribophorin IA;
DE Short=RPN-IA;
DE AltName: Full=Ribophorin-1A;
DE Flags: Precursor;
GN Name=OST1A; Synonyms=RPN1A; OrderedLocusNames=Os05g0301500, LOC_Os05g23600;
GN ORFNames=OsJ_17960;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC catalyzes the initial transfer of a defined glycan
CC (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC consensus motif in nascent polypeptide chains, the first step in
CC protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC the complex associates with the Sec61 complex at the channel-forming
CC translocon complex that mediates protein translocation across the
CC endoplasmic reticulum (ER). All subunits are required for a maximal
CC enzyme activity. {ECO:0000250|UniProtKB:P41543}.
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC {ECO:0000250|UniProtKB:P41543}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass type I membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the OST1 family. {ECO:0000305}.
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DR EMBL; AP008211; BAF17048.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS93226.1; -; Genomic_DNA.
DR EMBL; CM000142; EEE63151.1; -; Genomic_DNA.
DR EMBL; AK070329; BAG91888.1; -; mRNA.
DR RefSeq; XP_015640190.1; XM_015784704.1.
DR AlphaFoldDB; Q0DJC5; -.
DR SMR; Q0DJC5; -.
DR STRING; 4530.OS05T0301500-01; -.
DR PaxDb; Q0DJC5; -.
DR PRIDE; Q0DJC5; -.
DR EnsemblPlants; Os05t0301500-01; Os05t0301500-01; Os05g0301500.
DR GeneID; 4338348; -.
DR Gramene; Os05t0301500-01; Os05t0301500-01; Os05g0301500.
DR KEGG; osa:4338348; -.
DR eggNOG; KOG2291; Eukaryota.
DR HOGENOM; CLU_031381_2_0_1; -.
DR InParanoid; Q0DJC5; -.
DR OMA; FVKYVGN; -.
DR OrthoDB; 1294725at2759; -.
DR UniPathway; UPA00378; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000007752; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR ExpressionAtlas; Q0DJC5; baseline and differential.
DR Genevisible; Q0DJC5; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008250; C:oligosaccharyltransferase complex; IBA:GO_Central.
DR GO; GO:0018279; P:protein N-linked glycosylation via asparagine; IBA:GO_Central.
DR InterPro; IPR007676; Ribophorin_I.
DR PANTHER; PTHR21049; PTHR21049; 1.
DR Pfam; PF04597; Ribophorin_I; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Endoplasmic reticulum; Glycoprotein; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..615
FT /note="Dolichyl-diphosphooligosaccharide--protein
FT glycosyltransferase subunit 1A"
FT /id="PRO_0000420809"
FT TOPO_DOM 29..437
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..455
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 456..615
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 187
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250"
FT CARBOHYD 300
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 353
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 431
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 615 AA; 69522 MW; B5248D361070F22B CRC64;
MATPPPLRRV AALLLLLVAA ASTPTARADL VVTRADRKVD LTSHIVRVLT SLKVENSGPE
AVSQFLLAFP NVQAKNLAAI RAFGTEEKVK GPSMVLPIEV VQPSGVPPEL TFFSASLSKP
LEKGKTLHLD VLTVFTHSVQ PFPEEITQAE SQLVVYQDSA QYLSPYPVKV QTLSIRLPGG
RVESYTKYPN TKLAESELKY GPYEDLPPFS YSPMVVHYEN NNPFAVAKEV IREIEISHWG
NVQITEHYNI AHGGAKLKGE FSRIDYQSRP YIRGVSSFRH LIARLPPRAH SIYYRDEIGN
ISTSHLWSDS KKTQLEVEPR FPLFGGWQTT FTIGYGLPLQ DFVFNSDGKR FLNITFGSPV
EEILIEKLIV KVVLPEGSKD IDISVPFPTK QEQEVKYSHL DISGRPVVVL EKLDVIPEHN
LYFQVYYRFN NISLLREPMM LITGFFLLFM ACIVYMRTDM SISKNSPSYL AKVQWDEVQS
IIQQIQAIFN QCLAAHDKLE TSLHELSRSG DVKSCKVARK TADAQFKELA KELKPLLTSL
QSSSQSYQIW PKVEELVAKE RELQDKLMTR HSTVVDSFEK KLRGQDVENR IAAQQQKVAA
LRQEVESLLE YISEI
