OST3A_ARATH
ID OST3A_ARATH Reviewed; 343 AA.
AC F4I8X8; Q9LPY0;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3A;
DE Flags: Precursor;
GN Name=OST3A; OrderedLocusNames=At1g11560; ORFNames=T23J18.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC catalyzes the initial transfer of a defined glycan
CC (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC consensus motif in nascent polypeptide chains, the first step in
CC protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC the complex associates with the Sec61 complex at the channel-forming
CC translocon complex that mediates protein translocation across the
CC endoplasmic reticulum (ER). All subunits are required for a maximal
CC enzyme activity. {ECO:0000250|UniProtKB:P48439}.
CC -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC {ECO:0000250|UniProtKB:P48439}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the OST3/OST6 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF16635.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC011661; AAF16635.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28751.1; -; Genomic_DNA.
DR PIR; H86248; H86248.
DR RefSeq; NP_172622.1; NM_101029.2.
DR AlphaFoldDB; F4I8X8; -.
DR SMR; F4I8X8; -.
DR BioGRID; 22939; 1.
DR STRING; 3702.AT1G11560.1; -.
DR PaxDb; F4I8X8; -.
DR PRIDE; F4I8X8; -.
DR ProteomicsDB; 226037; -.
DR EnsemblPlants; AT1G11560.1; AT1G11560.1; AT1G11560.
DR GeneID; 837699; -.
DR Gramene; AT1G11560.1; AT1G11560.1; AT1G11560.
DR KEGG; ath:AT1G11560; -.
DR Araport; AT1G11560; -.
DR TAIR; locus:2200086; AT1G11560.
DR eggNOG; KOG2603; Eukaryota.
DR HOGENOM; CLU_046478_0_0_1; -.
DR InParanoid; F4I8X8; -.
DR OMA; CEIESTH; -.
DR OrthoDB; 1460433at2759; -.
DR PRO; PR:F4I8X8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I8X8; baseline and differential.
DR Genevisible; F4I8X8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008250; C:oligosaccharyltransferase complex; IBA:GO_Central.
DR InterPro; IPR021149; OligosaccharylTrfase_OST3/OST6.
DR PANTHER; PTHR12692; PTHR12692; 1.
DR Pfam; PF04756; OST3_OST6; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Glycoprotein; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..343
FT /note="Probable dolichyl-diphosphooligosaccharide--protein
FT glycosyltransferase subunit 3A"
FT /id="PRO_0000420815"
FT TOPO_DOM 23..185
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..220
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..273
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..304
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..343
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 105
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 108
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 146
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 343 AA; 39162 MW; 6B6F16583507C8FA CRC64;
MVIQTNLSYR FFILIVFLFT LANPKSDSDL KNELVSLRST AESGVISFNN DDVSKFITSV
STPRPYSLII FFDAVHLHGN SQLRLPEFRR EFRLVSATFI TNNNNESNGT KLFFCEIEST
HSEASFRRFA VESLPHISLV SPTTENLTES DQMDGGDFTG LAESMAEFVE RQTKLTVCSI
QRPPLISKTQ IGIIVAIIII STPILIKKIL KGETLLHDHR IWLVGAVFVY FFSVSGTMHN
IIREMPMYIK DYEDSSKFVF FIEESEMQLG AEGFFVGFLY TVVGLLLAFV TNVVVRVKKL
DEQRMAMLLA LSISFWAVRK VVYLDNWKTG YEIYPYWPSS WRG
