OST3B_ARATH
ID OST3B_ARATH Reviewed; 346 AA.
AC Q9SYB5; Q67ZL6;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B;
DE Flags: Precursor;
GN Name=OST3B; OrderedLocusNames=At1g61790; ORFNames=T13M11.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 309-346.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC catalyzes the initial transfer of a defined glycan
CC (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC consensus motif in nascent polypeptide chains, the first step in
CC protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC the complex associates with the Sec61 complex at the channel-forming
CC translocon complex that mediates protein translocation across the
CC endoplasmic reticulum (ER). All subunits are required for a maximal
CC enzyme activity. {ECO:0000250|UniProtKB:P48439}.
CC -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC {ECO:0000250|UniProtKB:P48439}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the OST3/OST6 family. {ECO:0000305}.
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DR EMBL; AC005882; AAD21423.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33886.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58452.1; -; Genomic_DNA.
DR EMBL; AK176101; BAD43864.1; -; mRNA.
DR PIR; G96643; G96643.
DR RefSeq; NP_001320885.1; NM_001334000.1.
DR RefSeq; NP_176372.1; NM_104861.2.
DR AlphaFoldDB; Q9SYB5; -.
DR SMR; Q9SYB5; -.
DR BioGRID; 27699; 3.
DR STRING; 3702.AT1G61790.1; -.
DR PaxDb; Q9SYB5; -.
DR PRIDE; Q9SYB5; -.
DR ProteomicsDB; 226038; -.
DR EnsemblPlants; AT1G61790.1; AT1G61790.1; AT1G61790.
DR EnsemblPlants; AT1G61790.2; AT1G61790.2; AT1G61790.
DR GeneID; 842476; -.
DR Gramene; AT1G61790.1; AT1G61790.1; AT1G61790.
DR Gramene; AT1G61790.2; AT1G61790.2; AT1G61790.
DR KEGG; ath:AT1G61790; -.
DR Araport; AT1G61790; -.
DR TAIR; locus:2195743; AT1G61790.
DR eggNOG; KOG2603; Eukaryota.
DR HOGENOM; CLU_046478_0_0_1; -.
DR InParanoid; Q9SYB5; -.
DR OMA; NLWAAVS; -.
DR OrthoDB; 1460433at2759; -.
DR PhylomeDB; Q9SYB5; -.
DR PRO; PR:Q9SYB5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SYB5; baseline and differential.
DR Genevisible; Q9SYB5; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0008250; C:oligosaccharyltransferase complex; IPI:TAIR.
DR GO; GO:0010483; P:pollen tube reception; IMP:TAIR.
DR GO; GO:0006487; P:protein N-linked glycosylation; IMP:TAIR.
DR InterPro; IPR021149; OligosaccharylTrfase_OST3/OST6.
DR PANTHER; PTHR12692; PTHR12692; 1.
DR Pfam; PF04756; OST3_OST6; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycoprotein; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..346
FT /note="Probable dolichyl-diphosphooligosaccharide--protein
FT glycosyltransferase subunit 3B"
FT /id="PRO_0000420816"
FT TOPO_DOM 23..189
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..225
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..277
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 299..308
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 330..346
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 108
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 346 AA; 38806 MW; 730BC639A8974A23 CRC64;
MALKSKLVSL LFLIATLSST FAASFSDSDS DSDLLNELVS LRSTSESGVI HLDDHGISKF
LTSASTPRPY SLLVFFDATQ LHSKNELRLQ ELRREFGIVS ASFLANNNGS EGTKLFFCEI
EFSKSQSSFQ LFGVNALPHI RLVSPSISNL RDESGQMDQS DYSRLAESMA EFVEQRTKLK
VGPIQRPPLL SKPQIGIIVA LIVIATPFII KRVLKGETIL HDTRLWLSGA IFIYFFSVAG
TMHNIIRKMP MFLQDRNDPN KLVFFYQGSG MQLGAEGFAV GFLYTVVGLL LAFVTNVLVR
VKNITAQRLI MLLALFISFW AVKKVVYLDN WKTGYGIHPY WPSSWR
