ID   OST3_ORYSJ              Reviewed;         336 AA.
AC   Q94I55; A0A0P0VZB7;
DT   09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3;
DE   Flags: Precursor;
GN   Name=OST3; OrderedLocusNames=Os03g0565100, LOC_Os03g36730;
GN   ORFNames=OSJNBa0084C09.17;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC       catalyzes the initial transfer of a defined glycan
CC       (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC       pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC       consensus motif in nascent polypeptide chains, the first step in
CC       protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC       the complex associates with the Sec61 complex at the channel-forming
CC       translocon complex that mediates protein translocation across the
CC       endoplasmic reticulum (ER). All subunits are required for a maximal
CC       enzyme activity. {ECO:0000250|UniProtKB:P48439}.
CC   -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC       {ECO:0000250|UniProtKB:P48439}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the OST3/OST6 family. {ECO:0000305}.
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DR   EMBL; AC016781; AAK53863.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF97146.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF12412.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS84949.1; -; Genomic_DNA.
DR   EMBL; AK061364; BAG87878.1; -; mRNA.
DR   EMBL; AK102421; BAG95548.1; -; mRNA.
DR   EMBL; AK109347; BAG98687.1; -; mRNA.
DR   RefSeq; XP_015632716.1; XM_015777230.1.
DR   AlphaFoldDB; Q94I55; -.
DR   SMR; Q94I55; -.
DR   STRING; 4530.OS03T0565100-01; -.
DR   PaxDb; Q94I55; -.
DR   PRIDE; Q94I55; -.
DR   EnsemblPlants; Os03t0565100-01; Os03t0565100-01; Os03g0565100.
DR   GeneID; 4333265; -.
DR   Gramene; Os03t0565100-01; Os03t0565100-01; Os03g0565100.
DR   KEGG; osa:4333265; -.
DR   eggNOG; KOG2603; Eukaryota.
DR   HOGENOM; CLU_046478_0_0_1; -.
DR   InParanoid; Q94I55; -.
DR   OMA; NLWAAVS; -.
DR   OrthoDB; 1460433at2759; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q94I55; OS.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008250; C:oligosaccharyltransferase complex; IBA:GO_Central.
DR   InterPro; IPR021149; OligosaccharylTrfase_OST3/OST6.
DR   PANTHER; PTHR12692; PTHR12692; 1.
DR   Pfam; PF04756; OST3_OST6; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Membrane; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..336
FT                   /note="Probable dolichyl-diphosphooligosaccharide--protein
FT                   glycosyltransferase subunit 3"
FT                   /id="PRO_0000420817"
FT   TOPO_DOM        26..183
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        184..204
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        205..215
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        216..236
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        237..267
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        268..288
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        289..298
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        299..319
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        320..336
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   336 AA;  37591 MW;  7F7AE1988D3E5E8B CRC64;
     MESPPHPLLL LLTLLVAAGA ASAGADDLVA ELQSLRSRSP SGVIHLTDTS ITRFLSAPAP
     RPYSVLVFFD AASLHSKTDL HLPQLRREFA LLSASFLAHN PASADLFFAD IEFSESQHSF
     AQFGVNSLPH VRLVRPEHTR LAGSEQMDQS HFSRLADSMA EFVESRTGLE VGPIVRPPLV
     SRNQMILLVI LFLVSIPFLI KRIMDGETLF HDRRVWMAGA LFIYFFSVSG GMYGIIRHTP
     MFITDRSDPN KLVFFYQGSG MQLGAEGFAV GFLYTLVGLM IAMVTHLLVR VESLQIQRFT
     MLAVMIIGWW AVKKVILLDN WKTGYSIHTF WPSSWR