OST48_ORYSJ
ID OST48_ORYSJ Reviewed; 439 AA.
AC Q6ZLK0; A0A0P0X412;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit;
DE Short=Oligosaccharyl transferase 48 kDa subunit;
DE Flags: Precursor;
GN Name=OST48; OrderedLocusNames=Os07g0209000, LOC_Os07g10830;
GN ORFNames=OJ1136_A05.21;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC catalyzes the initial transfer of a defined glycan
CC (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC consensus motif in nascent polypeptide chains, the first step in
CC protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC the complex associates with the Sec61 complex at the channel-forming
CC translocon complex that mediates protein translocation across the
CC endoplasmic reticulum (ER). All subunits are required for a maximal
CC enzyme activity. {ECO:0000250|UniProtKB:P33767}.
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC {ECO:0000250|UniProtKB:P33767}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass type I membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DDOST 48 kDa subunit family. {ECO:0000305}.
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DR EMBL; AP003748; BAC82967.2; -; Genomic_DNA.
DR EMBL; AP008213; BAF21079.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT00579.1; -; Genomic_DNA.
DR EMBL; AK059111; BAG86896.1; -; mRNA.
DR RefSeq; XP_015646882.1; XM_015791396.1.
DR AlphaFoldDB; Q6ZLK0; -.
DR SMR; Q6ZLK0; -.
DR STRING; 4530.OS07T0209000-01; -.
DR PaxDb; Q6ZLK0; -.
DR PRIDE; Q6ZLK0; -.
DR EnsemblPlants; Os07t0209000-01; Os07t0209000-01; Os07g0209000.
DR GeneID; 4342702; -.
DR Gramene; Os07t0209000-01; Os07t0209000-01; Os07g0209000.
DR KEGG; osa:4342702; -.
DR eggNOG; KOG2754; Eukaryota.
DR HOGENOM; CLU_031804_0_0_1; -.
DR InParanoid; Q6ZLK0; -.
DR OMA; YQFKVDY; -.
DR OrthoDB; 975158at2759; -.
DR PlantReactome; R-OSA-9030654; Primary root development.
DR UniPathway; UPA00378; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR Genevisible; Q6ZLK0; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008250; C:oligosaccharyltransferase complex; IBA:GO_Central.
DR GO; GO:0018279; P:protein N-linked glycosylation via asparagine; IBA:GO_Central.
DR InterPro; IPR005013; DDOST_48_kDa_subunit.
DR PANTHER; PTHR10830; PTHR10830; 1.
DR Pfam; PF03345; DDOST_48kD; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..439
FT /note="Dolichyl-diphosphooligosaccharide--protein
FT glycosyltransferase 48 kDa subunit"
FT /id="PRO_0000420814"
FT TOPO_DOM 25..416
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 438..439
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 439 AA; 48390 MW; BA5F722D7DD357B7 CRC64;
MAAPRHHHLA LAVALALLVV TAAAADEGGP RGRRVLVLVD DLAVRSSHSA FFASLQGRGF
DLDFRLADDP KLSLHRYGQY LYDGLVLFAP STPRFGGSVD QNSILEFIDA GHDMILAADS
SASDLIRGIA TECGVDFDED PEAMVIDHIN YAATDAEGDH TLIAGDDLIQ SDVILGSKKI
EAPVLFRGIG HAVNPSNSLV LKVLSASPSA YSANPKSKLA SPPSLTGSAI SLVSVMQARN
NARVLISGSL DLFSNRFLKS GVQKAGSKIR HEKAGNEQFV TETSKWVFHE RGHLKAVNVK
HNKVGETNEP GMYRINDDLE YSVEIYEWSG TSWKPYVADD VQVQFYMMSP YVLKTLSTDK
KGVFSTSFKV PDVYGVFQFK VEYQRLGYTG LSLSKQIPVR PYRHNEYERF ITSAYPYYAA
SFSTMGAFFI FSFVYLYHK
