ID   OST4A_ARATH             Reviewed;          37 AA.
AC   Q9LHK3;
DT   09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A;
GN   Name=OST4A; OrderedLocusNames=At3g12587; ORFNames=MMF12.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT   features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT   clones.";
RL   DNA Res. 7:217-221(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC       catalyzes the initial transfer of a defined glycan
CC       (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC       pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC       consensus motif in nascent polypeptide chains, the first step in
CC       protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC       the complex associates with the Sec61 complex at the channel-forming
CC       translocon complex that mediates protein translocation across the
CC       endoplasmic reticulum (ER). All subunits are required for a maximal
CC       enzyme activity. {ECO:0000250|UniProtKB:Q99380}.
CC   -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC       {ECO:0000250|UniProtKB:Q99380}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type III membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the OST4 family. {ECO:0000305}.
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DR   EMBL; AP002044; BAB02249.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE75219.1; -; Genomic_DNA.
DR   EMBL; AY088815; AAM67259.1; -; mRNA.
DR   RefSeq; NP_001118617.1; NM_001125145.2.
DR   AlphaFoldDB; Q9LHK3; -.
DR   SMR; Q9LHK3; -.
DR   STRING; 3702.AT3G12587.1; -.
DR   PaxDb; Q9LHK3; -.
DR   PRIDE; Q9LHK3; -.
DR   EnsemblPlants; AT3G12587.1; AT3G12587.1; AT3G12587.
DR   GeneID; 6240849; -.
DR   Gramene; AT3G12587.1; AT3G12587.1; AT3G12587.
DR   KEGG; ath:AT3G12587; -.
DR   Araport; AT3G12587; -.
DR   TAIR; locus:4515103057; AT3G12587.
DR   eggNOG; ENOG502SDK8; Eukaryota.
DR   HOGENOM; CLU_186352_1_1_1; -.
DR   InParanoid; Q9LHK3; -.
DR   OrthoDB; 1644285at2759; -.
DR   PhylomeDB; Q9LHK3; -.
DR   PRO; PR:Q9LHK3; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LHK3; baseline and differential.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR018943; Oligosaccaryltransferase.
DR   InterPro; IPR044165; OST4_plant.
DR   InterPro; IPR036330; Ost4p_sf.
DR   PANTHER; PTHR28677; PTHR28677; 1.
DR   Pfam; PF10215; Ost4; 1.
DR   SUPFAM; SSF103464; SSF103464; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Membrane; Reference proteome; Signal-anchor;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..37
FT                   /note="Dolichyl-diphosphooligosaccharide--protein
FT                   glycosyltransferase subunit 4A"
FT                   /id="PRO_0000420818"
FT   TOPO_DOM        1..7
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        29..37
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   37 AA;  4197 MW;  C24272AE2F4099D2 CRC64;
     MIDDQDLGFI ANFLGIFIFA LVIAYHYVTA DPKYEAT