ID   ASBD_BACAN              Reviewed;          91 AA.
AC   A0A0J1I1I3; E9RDX8; E9RDX9; Q6HZY3; Q6KTW2; Q81RQ6;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2017, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Acyl carrier protein AsbD {ECO:0000305};
DE   AltName: Full=Petrobactin biosynthesis protein AsbD {ECO:0000305};
GN   Name=asbD {ECO:0000303|PubMed:17189355};
GN   OrderedLocusNames=GBAA_1984 {ECO:0000312|EMBL:AAT31103.1};
OS   Bacillus anthracis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [2]
RP   FUNCTION, PATHWAY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=Sterne;
RX   PubMed=17189355; DOI=10.1128/jb.01526-06;
RA   Lee J.Y., Janes B.K., Passalacqua K.D., Pfleger B.F., Bergman N.H., Liu H.,
RA   Haakansson K., Somu R.V., Aldrich C.C., Cendrowski S., Hanna P.C.,
RA   Sherman D.H.;
RT   "Biosynthetic analysis of the petrobactin siderophore pathway from Bacillus
RT   anthracis.";
RL   J. Bacteriol. 189:1698-1710(2007).
RN   [3]
RP   FUNCTION, PATHWAY, PHOSPHOPANTETHEINYLATION AT SER-40, AND MUTAGENESIS OF
RP   SER-40.
RC   STRAIN=Sterne;
RX   PubMed=17346033; DOI=10.1021/bi6023995;
RA   Pfleger B.F., Lee J.Y., Somu R.V., Aldrich C.C., Hanna P.C., Sherman D.H.;
RT   "Characterization and analysis of early enzymes for petrobactin
RT   biosynthesis in Bacillus anthracis.";
RL   Biochemistry 46:4147-4157(2007).
RN   [4]
RP   REVIEW.
RX   PubMed=27425635; DOI=10.1111/mmi.13465;
RA   Hagan A.K., Carlson P.E. Jr., Hanna P.C.;
RT   "Flying under the radar: The non-canonical biochemistry and molecular
RT   biology of petrobactin from Bacillus anthracis.";
RL   Mol. Microbiol. 102:196-206(2016).
CC   -!- FUNCTION: Involved in the biosynthesis of petrobactin, a catecholate
CC       siderophore that functions in both iron acquisition and virulence
CC       (PubMed:17189355, PubMed:17346033). Aryl-carrier protein that activates
CC       3,4-dihydroxybenzoate (3,4-DHBA) prior to its incorporation into
CC       petrobactin (PubMed:17346033). {ECO:0000269|PubMed:17189355,
CC       ECO:0000269|PubMed:17346033}.
CC   -!- PATHWAY: Siderophore biosynthesis; petrobactin biosynthesis.
CC       {ECO:0000269|PubMed:17189355, ECO:0000269|PubMed:17346033}.
CC   -!- PTM: Activated by the transfer of a 4'-phosphopantetheine group from
CC       CoA to Ser-40. {ECO:0000269|PubMed:17346033}.
CC   -!- DISRUPTION PHENOTYPE: The deletion mutant cannot produce petrobactin on
CC       iron-depleted medium. In vitro analysis show that mutants grow to a
CC       very limited extent as vegetative cells in iron-depleted medium but are
CC       not able to outgrow from spores under the same culture conditions.
CC       {ECO:0000269|PubMed:17189355}.
CC   -!- SIMILARITY: Belongs to the acyl carrier protein (ACP) family.
CC       {ECO:0000305}.
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DR   EMBL; AE017334; AAT31103.1; -; Genomic_DNA.
DR   RefSeq; WP_001250565.1; NZ_WXXJ01000029.1.
DR   AlphaFoldDB; A0A0J1I1I3; -.
DR   SMR; A0A0J1I1I3; -.
DR   STRING; 260799.BAS1841; -.
DR   DNASU; 1085887; -.
DR   EnsemblBacteria; AAT31103; AAT31103; GBAA_1984.
DR   GeneID; 45021906; -.
DR   KEGG; bar:GBAA_1984; -.
DR   PATRIC; fig|1392.230.peg.1944; -.
DR   eggNOG; COG0236; Bacteria.
DR   HOGENOM; CLU_108696_8_2_9; -.
DR   OMA; VYIEMDL; -.
DR   UniPathway; UPA01005; -.
DR   Proteomes; UP000000594; Chromosome.
DR   Gene3D; 1.10.1200.10; -; 1.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   SUPFAM; SSF47336; SSF47336; 1.
DR   PROSITE; PS50075; CARRIER; 1.
PE   1: Evidence at protein level;
KW   Phosphopantetheine; Phosphoprotein; Reference proteome.
FT   CHAIN           1..91
FT                   /note="Acyl carrier protein AsbD"
FT                   /id="PRO_0000450622"
FT   DOMAIN          4..82
FT                   /note="Carrier"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   MOD_RES         40
FT                   /note="O-(pantetheine 4'-phosphoryl)serine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258,
FT                   ECO:0000269|PubMed:17346033"
FT   MUTAGEN         40
FT                   /note="S->A: Loss of phosphopantetheinylation."
FT                   /evidence="ECO:0000269|PubMed:17346033"
SQ   SEQUENCE   91 AA;  10628 MW;  8762C1564E2A2BE8 CRC64;
     MRREALKNAV LKIMTEKMEL KNVTHLEETM RLNQDLYIDS VMMLQLIVYI EMDVKLCVPE
     DEVDPKAFLT VGSLLDFMEE LQPLQDVNVN N